The increasing amount of plastic waste causes significant environmental pollution. In this study, screening Arctic microorganisms which are able to degrade bioplastics was performed. total, 313 were isolated from 52 soil samples the region (Spitsbergen). Among microorganisms, 121 (38.66%) showed biodegradation activity. ability clear zone formation on emulsified poly(butylene succinate-co-adipate) (PBSA) observed for 116 (95.87%), succinate) (PBS) 73 (60.33%), and poly(ɛ-caprolactone) (PCL)...

Recently it was demonstrated that mealworm (Tenebrio molitor) larvae consume and biodegrade polystyrene. Thus, in this study a breeding investigation with various types of polystyrene performed to follow the changes gut microbiome diversity. Polystyrene used for packaging purposes (PSp) expanded (EPS) were perceived as more favorable attacked frequently by mealworms compared raw (PS) material commercially available parcels (PSp). Although our studies showed could bite chew selected...

Erythritol, a four-carbon polyol synthesized by microorganisms as an osmoprotectant, is natural sweetener produced on industrial scale for decades. Despite the fact that yeast Yarrowia lipolytica has been reported since 1970s erythritol producer, metabolic pathway of this never characterized. It was shown synthesis in occurs via pentose phosphate (PPP). The oleaginous Y. good host converting inexpensive glycerol into value-added product such erythritol. Glycerol renewable feedstock which...

Increasing interest of non-conventional yeasts has been observed for many years due to their biochemical characteristics and potential applications. Well-studied, oleaginous yeast Y. lipolytica is an attractive host converting a low-cost glycerol, into value-added products such as erythritol (sweetener) or citric acid. Glycerol important renewable feedstock the main co-product biodiesel production, which nowadays applied on large commercial scale. To this end, we engineered increase...

Erythritol is a natural sweetener that used in the food industry. It produced as an osmoprotectant by bacteria and yeast. Due to its chemical properties, it does not change insulin level blood, therefore can be safely diabetics. Previously, has been shown erythrose reductase (ER), which catalyzes final step, plays crucial role erythritol synthesis. ER reduces with NAD(P)H cofactor. Despite many studies on synthesis Yarrowia lipolytica, enzymes involved this metabolic pathway have ever...

In this study, the scale-up of erythritol production from glycerol using Yarrowia lipolytica strain MK1 is proposed. The experiments included increasing scale laboratory (VW = 0.002 m3) to pilot plant (VP 0.5 m3). Only slight differences in rate were observed between two culture variants. Erythritol concentration experiment reached 180.3 kg/m3, with productivity 1.25 kg/m3·h and yield 0.533 kg/kg total amount consumed. A model an industrial installation for crystals, 1075 MT (Metric...

There has been a growing interest in poly(ethylene terephthalate) PET degradation studies the last few years due to its widespread use and large-scale plastic waste accumulation environment. One of most promising enzymatic methods context is PETase from Ideonella sakaiensis, which reported be an efficient enzyme for hydrolysing ester bonds PET. In our study, we expressed codon-optimized gene yeast Yarrowia lipolytica. The obtained strain was tested ability degrade directly culture, screening...

The global limitation of fossil fuels impels scientists to search for new energy sources. A good alternative is biodiesel produced from crop plants. However, its production requires huge quantities farmland, fertilizers and fresh water, which in conflict with the human demand water consumption land food production. Thus, single cell oil (SCO) by oleaginous microorganisms remains best solution coming years. Whereas most require proper metabolism, this study we demonstrate that unconventional...

Yarrowia lipolytica is an unconventional yeast with a huge industrial potential. Despite many advantages for biotechnological applications, it possesses enormous demand oxygen, which bottleneck in large scale production. In this study codon optimized bacterial hemoglobin from Vitreoscilla stercoraria (VHb) was overexpressed Y. efficient growth and erythritol synthesis glycerol low-oxygen conditions. Erythritol natural sweetener produced by under high osmotic pressure at low pH, process...

The aim of this study was to test rye straw, bran and oat hydrolysates as substrates for growth the yeast Yarrowia lipolytica, a microorganism known have large biotechnological potential. First, after combined process acid-enzymatic hydrolysis, concentration composition fermentable monosaccharides in obtained were analyzed. Glucose main sugar, followed by xylose arabinose. Rye hydrolysate had highest sugar content—80.8 g/L. results showed that able grow on low-cost medium produce biomass...

Polyethylene terephthalate (PET) is the most widely used plastic, whose global production scale causes serious problems due to it being highly non-biodegradable. The present work provides a novel approach plastic degradation studies, which involves direct of PET in culture modified Y. lipolytica yeast strain extracellularly producing cutinase from Fusarium solani. In this study, we successfully accomplished scale-up process culture, promising perspective wider application developed method...

Abstract Background Yarrowia lipolytica possesses the capability to utilize many unconventional carbon sources, such as crude glycerol, alkanes and fatty acids. Despite producing polyols, erythritol, arabitol mannitol, re-utilization of mannitol is not efficient erythritol utilization. Genes involved in uptake metabolism Y. remain undescribed. However, deletion EYD1 gene ( YALI0F01650g ), believed encode dehydrogenase, has been found result a high rate growth on media containing sole source....

The purpose of this study was to assess the ability Bacillus aryabhattai RAF 5 produce P(3HB) under conditions periodic medium renewal (PMR). polymer isolated and confirmed using transmission electron microscopy (TEM), which revealed presence more than 10 dense granules per cell. purity evaluated Fourier transform infrared spectroscopy (FTIR). maximum concentration reached 18.70 g/L with biomass accumulation 21.44 after 120 h incubation PMR conditions, is 6.61 higher obtained standard...