Increasing interest of non-conventional yeasts has been observed for many years due to their biochemical characteristics and potential applications. Well-studied, oleaginous yeast Y. lipolytica is an attractive host converting a low-cost glycerol, into value-added products such as erythritol (sweetener) or citric acid. Glycerol important renewable feedstock the main co-product biodiesel production, which nowadays applied on large commercial scale. To this end, we engineered increase...

The global limitation of fossil fuels impels scientists to search for new energy sources. A good alternative is biodiesel produced from crop plants. However, its production requires huge quantities farmland, fertilizers and fresh water, which in conflict with the human demand water consumption land food production. Thus, single cell oil (SCO) by oleaginous microorganisms remains best solution coming years. Whereas most require proper metabolism, this study we demonstrate that unconventional...

Abstract Background Yarrowia lipolytica possesses the capability to utilize many unconventional carbon sources, such as crude glycerol, alkanes and fatty acids. Despite producing polyols, erythritol, arabitol mannitol, re-utilization of mannitol is not efficient erythritol utilization. Genes involved in uptake metabolism Y. remain undescribed. However, deletion EYD1 gene ( YALI0F01650g ), believed encode dehydrogenase, has been found result a high rate growth on media containing sole source....

The gene YALI0F01562g was identified as an important factor involved in erythritol catabolism of the unconventional yeast Yarrowia lipolytica. Its putative role for first time by comparative analysis four Y. lipolytica strains: A-101.1.31, Wratislavia K1, MK1 and AMM. presence a mutation that seriously damaged corresponded to inability strain K1 utilize erythritol. RT-PCR demonstrated significant increase expression during growth on Further studies involving deletion overexpression selected...

Erythritol is a polyol produced by Yarrowia lipolytica under hyperosmotic stress. In this study, the osmo-sensitive strain Y. yl-hog1Δ was subjected to stress, triggered high concentration of carbon sources. The thrived on 0.75 M erythritol medium, while same concentrations glucose and glycerol proved be lethal. addition 0.1 medium containing or allowed growth yl-hog1Δ. Supplementation with other potential osmolytes such as mannitol L-proline did not have similar effect. To examine whether...

Abstract Erythritol production is a unique response to hyperosmotic stress that observed in small group of yeasts, including Yarrowia lipolytica . This study investigated whether this unusual mechanism regulated by the HOG pathway, well described Saccharomyces cerevisiae The gene YALI0E25135g was identified as Y. homologue HOG1 and found be phosphorylated shock. Deletion caused significant decrease resistance negatively affected erythritol production. Interestingly, deletion strain yl-hog1Δ...

Erythritol is a natural sweetener produced by microorganisms as an osmoprotectant. It belongs to the group of polyols and it can be utilized oleaginous yeast Yarrowia lipolytica. Despite recent identification transcription factor erythritol utilization (EUF1), metabolic pathway catabolism remains unknown. In this study we identified new gene, YALI0F01628g, involved in assimilation. silico analysis showed that YALI0F01628g putative isomerase localized same region EUF1. qRT-PCR Y. lipolytica...

Abstract The unconventional yeast Yarrowia lipolytica produces erythritol as an osmoprotectant to adapt osmotic stress. In this study, the array of putative erythrose reductases, responsible for conversion d-erythrose erythritol, was analyzed. Single knockout and multiple strains were tested their ability produce polyols in stress conditions. Lack six reductase genes does not affect significantly, production polyol is comparable control strain. Deletion eight homologous resulted a 91%...

The unconventional yeast Yarrowia lipolytica is used to produce erythritol from glycerol. In this study, the role of erythrose reductase (ER) homolog YALI0B07117g in synthesis was analyzed. deletion gene resulted an increased production mannitol (308%) and arabitol (204%) before utilization these polyols began. strain overexpressing increase yield glycerol as a sole carbon source batch cultures, resulting 0.4 g/g. specific consumption rate (qs) 5.83 g/g/L for WT 8.49 modified productivity...

Yarrowia lipolytica is a dimorphic fungus, which switches from yeast to filament form in response environmental conditions. For industrial purposes it important lock cells the or filamentous depending on fermentation process. yl-Hog1 kinase key component of HOG signaling pathway, responsible for activating osmotic stress response. Additionally, deletion leads increased filamentation lipolytica, but causes significant sensitivity induced by high concentration carbon source.In this study, we...

Abstract Background Erythritol is a four-carbon polyol with an unclear role in metabolism of some unconventional yeasts. Its production has been linked to the osmotic stress response, but mechanism protection remains unclear. Additionally, erythritol can be used as carbon source. In yeast Yarrowia lipolytica , its assimilation activated by transcription factor Euf1. The study investigates whether this link other processes cell. Results research was performed on two closely related strains Y....

Yarrowia lipolytica possesses the capability to utilize many unconventional carbon sources, and deletion of YALI0F01650g has been found result in a high rate growth on media containing mannitol as sole source. Therefore this feature was used for fermentation studies macroalgal extracts. Production biomass titer led overexpression DGA1 gene elevated lipid content yeast biomass. The obtained strain AJD ΔEYD1 able take up efficiently produce higher amounts lipids, reaching almost 32% overall...

Abstract BackgroundPolyols are a group of sweet alcohols, frequently used as food additives. The constantly rising demand for polyols requires the application new strategies to increase production. Erythritol is synthesized by yeast Yarrowia lipolytica under high osmotic pressure an osmoprotectant. metabolic pathway resulting in erythritol production remains partially unknown. However, last reaction synthesis conducted erythrose reductase (ER).ResultsThe Y. strain was genetically modified...

Abstract The unconventional yeast Yarrowia lipolytica produces erythritol as a response to osmotic stress. In this study, the array of putative erythrose reductases, responsible for conversion d-erythrose erythritol, was analyzed. Single knockout and multiple strains were tested their ability produce polyols in stress conditions. Lack six reductase genes does not affect significantly, production polyol is comparable control strain. Deletion eight homologous resulted 91% decrease synthesis,...

Abstract Background Yarrowia lipolytica is a dimorphic fungus, which switches from yeast to yeast-to-filament form in response environmental conditions. For industrial purposes it important lock cells the or filamentous depending on fermentation process. yl-Hog1 kinase key component of HOG signaling pathway, responsible for activating osmotic stress response. Additionally, deletion leads increased filamentation , but causes significant sensitivity induced by high concentration carbon source....