- Microbial bioremediation and biosurfactants
- biodegradable polymer synthesis and properties
- Microplastics and Plastic Pollution
- Microbial Metabolic Engineering and Bioproduction
- Antifungal resistance and susceptibility
- Enzyme Production and Characterization
- Recycling and Waste Management Techniques
- Toxic Organic Pollutants Impact
- Cancer Research and Treatments
- Plant Pathogens and Fungal Diseases
- Advanced Drug Delivery Systems
- Plant-Microbe Interactions and Immunity
- Enzyme Catalysis and Immobilization
- Bacterial biofilms and quorum sensing
- Mass Spectrometry Techniques and Applications
- Algal biology and biofuel production
- Bacterial Genetics and Biotechnology
- Antimicrobial agents and applications
- Biodiesel Production and Applications
- Microbial Natural Products and Biosynthesis
- Analytical Chemistry and Chromatography
- Inhalation and Respiratory Drug Delivery
- Nematode management and characterization studies
- Cystic Fibrosis Research Advances
Wroclaw University of Environmental and Life Sciences
2020-2022
Łukasiewicz Research Network – PORT Polish Center for Technology Development
2021
University of Wrocław
2013-2020
Most biosurfactants are obtained using costly culture media and purification processes, which limits their wider industrial use. Sustainability of production processes can be achieved, in part, by cheap substrates found among agricultural food wastes or byproducts. In the present study, crude glycerol, a raw material from several was evaluated as potential low-cost carbon source to reduce costs surfactin Bacillus subtilis #309. The medium containing soap-derived waste glycerol led best...
Polyethylene terephthalate (PET) is the most widely used plastic, whose global production scale causes serious problems due to it being highly non-biodegradable. The present work provides a novel approach plastic degradation studies, which involves direct of PET in culture modified Y. lipolytica yeast strain extracellularly producing cutinase from Fusarium solani. In this study, we successfully accomplished scale-up process culture, promising perspective wider application developed method...
A serious problem for humans is the propensity of Candida albicans to adhere various surfaces and its ability form biofilms. Surfactants or biosurfactants can affect cell microorganisms block their adhesion different substrates. This study investigated C. strains differing in surface hydrophobicity (CSH) polystyrene microplates order compare lipopeptide pseudofactin (PF II) surfactin (SU) prevent fungal polystyrene. The decreased tested by 35-90 % when were conditioned before addition cells....
Lipopeptides are a promising group of surface-active compounds microbial origin (biosurfactants). These diverse molecules produced mainly by Bacillus and Pseudomonas strains. Because their attractive physiochemical biological properties, biosurfactants considered to be "green versatile the future". The main obstacles in widespread use low yields high production costs. Pseudofactin (PF) is lipopeptide fluorescens BD5. Recently, we identified two analogues, PF1 (C16-Val) PF2 (C16-Leu),...
The rapid and accurate quantification of biosurfactants in biological samples is challenging. In contrast to the orcinol method for rhamnolipids, no simple biochemical available lipopeptides. Various liquid chromatography (LC) methods are promising tools relatively fast exact Here, we report strategies lipopeptides pseudofactin surfactin bacterial cultures using different high- (HPLC) ultra-performance (UPLC) systems. We tested three sample pretreatment prior LC analysis. direct analysis...
Microbial surfactants (biosurfactants) have gained interest as promising substitutes of synthetic surface-active compounds. However, their production and purification are still challenging, with significant room for efficiency costs optimization. In this work, we introduce a method the enhanced cyclic lipopeptides pseudofactins (PFs) from Pseudomonas fluorescens BD5 cultures. The is directly applicable in technical scale possibility further upscaling. Comparing to original protocol PFs...
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