A5043907792- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Cancer therapeutics and mechanisms
- Mycobacterium research and diagnosis
- Advanced biosensing and bioanalysis techniques
- Biochemical and Molecular Research
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Monoclonal and Polyclonal Antibodies Research
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Tuberculosis Research and Epidemiology
- Phytase and its Applications
- Enterobacteriaceae and Cronobacter Research
- Toxin Mechanisms and Immunotoxins
- Bioactive Compounds and Antitumor Agents
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Microbial Metabolism and Applications
- Oral and gingival health research
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Cellular Mechanics and Interactions
National Center for Biotechnology
2014-2025
S.Seifullin Kazakh Agro Technical University
2023
National Nuclear Center of the Republic of Kazakhstan
2022
Alternaria fungal species are considered major plant pathogens, infecting various crops and resulting in significant agricultural losses. Additionally, these can contaminate grain with multiple mycotoxins that harmful to humans animals. Efficient pest management relies on timely detection identification of phytopathogens samples, facilitating prompt selection a crop protection strategy. Conventional tools, such as morphological characterization based polymerase chain reaction (PCR)-based...
The purpose of this study was to assess the ability Bacillus aryabhattai RAF 5 produce P(3HB) under conditions periodic medium renewal (PMR). polymer isolated and confirmed using transmission electron microscopy (TEM), which revealed presence more than 10 dense granules per cell. purity evaluated Fourier transform infrared spectroscopy (FTIR). maximum concentration reached 18.70 g/L with biomass accumulation 21.44 after 120 h incubation PMR conditions, is 6.61 higher obtained standard...
Mastitis is a prevalent disease affecting dairy cows, leading to significant economic losses in the industry. Conventional diagnostic methods such as microbiology and PCR are expensive time-consuming, emphasizing need for alternative approaches. The field of novel diagnostics expanding rapidly due application modern molecular detection based on CRISPR/Cas system. This system functions by targeting specific genetic sequences pathogen, including Escherichia coli, detects presence pathogen...
Apurinic/apyrimidinic (AP) endonucleases play critical roles in the repair of abasic sites and strand breaks DNA. Complete genome sequences Helicobacter pylori reveal that this bacterial specie has a single AP endonuclease. An H. homolog Xth (HpXth) is member exonuclease III family, which represented by Escherichia coli Xth. Currently, it remains unknown whether endonuclease DNA activities similar to those its counterpart E. other bacteria. We report HpXth possesses efficient site cleavage,...
Human epidermal growth factor receptor 2 (HER2) is an important biomarker for detection and treatment of different types cancers such as breast, ovarian, stomach cancer. In this study, we developed a monoclonal antibody against the extracellular d
In this work, the strains Bacillus megaterium RAZ 3, Azotobacter chrocococcum Az araybhattay RA 5 were used as an effective producer of poly-3-hydroxybutyrate P(3HB). The purpose study was to isolate and obtain P(3HB) isolated from regional chestnut soils northern Kazakhstan. This demonstrates possibility combining protective system cells physical stress a way optimize synthesis PHA by strains. Molecular identification amplification phbC gene, transmission electron microscope (TEM),...
According to the World Health Organization, Kazakhstan takes a leading position in number of cases multidrug-resistant tuberculosis. This work is aimed at studying clinical isolates Mycobacterium tuberculosis circulating Astana 2021–2022. Among 198 M. collected, 175 (88.38%) belonged L2/Beijing family, and remaining 23 samples (11.62%) L4 family. During research used genotyping determining genetic family tuberculosis, as well whole-genome sequencing selected 24 with their subsequent...
During
Hydrogels used in biomedical applications, particularly for soft tissue treatment and regeneration, should have favorable mechanical properties biocompatibility. Alginate hydrogels are suitable developing wound dressings with desirable porosity, facilitating effective gas vapor exchange necessary healing. In this study, we present the synthesis characterization of photocrosslinked based on sodium alginate allyl glycidyl ether (SA-AGE). The composition structure synthesized macromers were...
Molecular diagnostics is widely recognized as one of the most efficient approaches for detecting and characterizing microorganisms. This method relies on identification specific nucleic acid sequences enables quick precise determination pathogen presence in various samples. Conventional methods detection rely culture a laboratory setting, which can be time-consuming expensive. diagnostic methods, such PCR DNA sequencing, have emerged powerful alternatives to culture-based offering greater...
Introduction: Tuberculosis (TB) is a human disease caused by Mycobacterium tuberculosis (Mtb). Treatment of TB requires long-term courses multi-drug therapies to eliminate subpopulations bacteria, which sometimes persist against antibiotics. Therefore, understanding the mechanism Mtb antibiotic-resistance extremely important. During infection, overcomes variety body defense mechanisms, including treatment with reactive species oxygen and nitrogen. The bases in DNA molecule are susceptible...
BACKGROUND: Active proliferation is specific property of a tumor cells. However, the cost analysis high due to commercial anti-Ki-67 mAbs used as main immunoreagent for reliable identification proliferating In this study, recombinant protein was obtain Ki-67 biomarker immunodetection.METHODS: Codon optimized fragment ki-67 gene cloned into pET28c(+)vector. The purified by immobilized metal affinity chromatography (IMAC) and confirmed liquid chromatography–mass spectrometry (LC-MS)/MS....
This study explores the utility of CRISPR/Cas-based diagnostics for precise identification Staphylococcus aureus (S. aureus), particularly when integrated with recombinase polymerase amplification (RPA). S. aureus, a versatile Gram-positive bacterium, presents significant challenges due to its antibiotic resistance and capacity cause variety infections. While existing diagnostic methods provide accuracy, they often involve time-consuming procedures require specialized equipment. Our research...