A5049178911- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Microbial Metabolites in Food Biotechnology
- Fermentation and Sensory Analysis
- Probiotics and Fermented Foods
- Fungal and yeast genetics research
- Yeasts and Rust Fungi Studies
- Biochemical Acid Research Studies
- Medicinal plant effects and applications
- Algal biology and biofuel production
- Agriculture, Plant Science, Crop Management
- Enzyme Production and Characterization
- Phytochemistry and Biological Activities
- Phytase and its Applications
- Phytochemical and Pharmacological Studies
- Essential Oils and Antimicrobial Activity
- Waste Management and Environmental Impact
- Plant nutrient uptake and metabolism
- Heavy Metals in Plants
- Chemical and Physical Properties in Aqueous Solutions
- Agriculture and Biological Studies
- Botany and Plant Ecology Studies
- Biodiesel Production and Applications
- Plant Pathogens and Resistance
Wroclaw University of Environmental and Life Sciences
2013-2025
University of Patras
2016
University of Wrocław
2001-2006
University of Agriculture in Krakow
2005
University of Business in Wrocław
2003
Erythritol is a polyol with sweet taste but low energy value. Thanks to its valuable properties, as well growing social awareness and nutritional trends, popularity rapidly. The aim of this study was increase the effectiveness erythritol production from glucose using new UV mutants yeast Yarrowia lipolytica obtained in Wratislavia K1 strain. ability strains biosynthesize utilize examined shake-flask cultures fed-batch processes conducted stirred tank reactor total concentration 300 400 g/L....
This study investigates the waste-free bioconversion of glucose to erythritol using UV-mutagenized strain Yarrowia lipolytica Wratislavia KI UV15. research focuses on optimizing fermentation parameters enhance yield, with a key emphasis utilizing post-crystallization filtrate as primary carbon source, promoting cost-effective and sustainable approach production. The experimental design included systematic variations in ammonium sulfate concentration, yeast extract supplementation, initial...
Natural flavonoids, such as naringenin, hesperetin, chrysin, apigenin, luteolin, quercetin, epicatechin, and biochanin A, were subjected to microbiological transformations by Rhodotorula glutinis. Yeast was able regioselectively C-8 hydroxylate chrysin. Naringenin transformed 8- 6-hydroxyderivatives. Quercetin, A did not undergo biotransformation. metabolic pathway for the degradation of chrysin has been elucidated. The metabolism proceeds via an initial hydroxylation norwogonin, followed...
The aim of the study was to identify and evaluate content iridoids phenolic compounds in leaves roots Dipsacus fullonum L. They were identified quantified by UPLC-PDA-MS/MS. Five iridoid (loganic acid, loganin, sweroside, cantleyoside, sylvestroside III) roots. Seven acids three flavones leaves, seven detected contained more than We also evaluated antimicrobial (anti-bacterial anti-yeast), antioxidant (ORAC methods), antiacetylcholinesterase (AChE) activities Leaf extract demonstrated...
Citric acid and erythritol are obtained on an industrial scale using biotechnological methods. Due to the growing market demand for these products, research is underway improve process economics by introducing new microorganisms, in particular of species Yarrowia lipolytica. The aim this study was evaluate transformants Y. lipolytica growth ability overproduce citric acids from glycerol. were constructed overexpressing glycerol kinase, methylcitrate synthase mitochondrial succinate-fumarate...
Yarrowia lipolytica is a non-conventional yeast producing valuable compounds, such as citric acids, from renewable raw materials. This study investigated the impact of citrate synthase overexpression on biosynthesis and isocitric acid in Y. lipolytica. Two transformants A101.1.31 strain (efficient producer), overexpressing CIT1 or CIT2 gene (encoding proteins with activity), were constructed. The results revealed that either these genes enhances activity. Additionally, cit1 knockout was...
The microbiological biosynthesis of α-ketoglutaric acid (KGA) has recently captured the attention many scientists as an alternative to its common chemical synthesis. present study aimed evaluate effect feeding strategy substrates, i.e., glycerol (G = 20 g·dm−3) and rapeseed oil (O g·dm−3), on yeast growth parameters KGA by a wild strain Yarrowia lipolytica A-8 in fed-batch repeated-batch cultures. effectiveness was demonstrated depend thiamine concentration substrate method. In culture...
Y. lipolytica remains a nonpathogenic, unconventional yeast, which can be applied for the production of bioactive compounds. Our previous study confirmed ability yeast Yarrowia to produce kynurenic acid (KYNA). Here, we investigated effectiveness KYNA in cultures cultivated medium containing honey various origin, used as source carbon and energy. It was evidenced that highest content culture broth (68 mg/L) biomass (542 mg/kg) obtained when chestnut used. The lipids amino acids composition...
Most of the world's annual production mannitol is by chemical means, but, due to increasing demand for natural sweeteners, alternative methods are being sought. The aim study was screen Yarrowia lipolytica yeast strains and select culture conditions efficient selective biosynthesis from glycerol. From 21 examined in shake-flask glycerol (100 g/L), three were selected-S2, S3, S4-and further evaluated batch bioreactor cultures with technical raw (150 g/L). best parameters observed strain which...
The present study aimed to develop a technology for the production of dietary supplements based on yeast biomass and α-ketoglutaric acid (KGA), produced by new transformant Yarrowia lipolytica with improved KGA biosynthesis ability, as well verify usefulness obtained products food feed purposes. Transformants Y. were constructed overexpress genes encoding glycerol kinase, methylcitrate synthase mitochondrial organic transporter. strains compared in terms growth ability glycerol- oil-based...
Abstract Enzymatic preparation from culture of keratinolytic Bacillus cereus PCM 2849 was applied for hydrolysis whole chicken feathers, after sulphitolytic pretreatment. This process optimized using a three-factor Box-Behnken design, where the effect substrate concentration, sulphite concentration during pretreatment and reaction temperature evaluated on release amino acids. Obtained results revealed highest impact temperature, followed by content Optimal conditions were established, i.e....