A5087866367- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Viral Infectious Diseases and Gene Expression in Insects
- 3D Printing in Biomedical Research
- Biofuel production and bioconversion
- Algal biology and biofuel production
- Microbial Metabolites in Food Biotechnology
- Transgenic Plants and Applications
University of Liège
2022-2023
Pontificia Universidad Católica de Valparaíso
2019-2023
Gembloux Agro-Bio Tech
2022-2023
Komagataella phaffii (aka Pichia pastoris) is a yeast able to grow in methanol as the sole carbon and energy source. This substrate converted into formaldehyde, toxic intermediary that can either be assimilated biomass or dissimilated CO2 through enzymes formaldehyde dehydrogenase (FLD) formate dehydrogenase, also producing form of NADH. The dissimilative pathway has been described an detoxifying route, but conclusive evidence not provided for this. In order elucidate this theory, we...
Abstract Pichia pastoris is a well‐established cell factory for recombinant protein synthesis. Various optimization strategies of processes based on AOX1 promoter have been investigated, including methanol co‐feeding with glycerol or sorbitol during the induction stage. Compared carbon sources, comparatively little research has devoted to effects nitrogen sources. Several reports described benefits adding casamino acids (CA) production medium, however, without considering its at gene...