Abstract Cancer drug development has been riddled with high attrition rates, in part, due to poor reproducibility of preclinical models for discovery. Poor experimental design and lack scientific transparency may cause biases that turn affect data quality, robustness reproducibility. Here, we pinpoint sources variability conventional 2D cell-based cancer screens determine the effect confounders on cell viability MCF7 HCC38 breast lines treated platinum agents (cisplatin carboplatin) a...

The factors inducing male offspring and the mechanism of sex—ratio adjustment were investigated in Daphnia magna, a cyclically parthenogenetic cladoceran (Crustacea). Laboratory experiments conducted on individual animals living flow—through chambers, to separate effects various density—dependent factors. Fluctuating food levels had no sex when starvation was not severe. Water from crowded populations different species contained substances that induced up 43% broods. chemically mediated...

Journal Article Nasopharyngeal Colonization during the First Year of Life Get access Gustaf Aniansson, Aniansson Department Clinical Immunology, Lund University, and Departments Pediatrics, ClinicalBacteriology, Otorhinolaryngology, Göteborg Sweden Search for other works by this author on: Oxford Academic PubMed Google Scholar Bernt Aim, Aim Bengt Andersson, Andersson Peter Larsson, Larsson Olle Nylen, Nylen Hans Peterson, Peterson Rigner, Rigner Marianne Svanborg, Svanborg Catharina The...

Rectal cultures from Swedish and Pakistani hospital-delivered newborn infants were analysed regarding the early acquisition of enterobacteria. delivered vaginally, either vaginally or by caesarean section. The all breast-fed, whereas breastfeeding was incomplete often started late among infants. Both groups more rapidly colonized with enterobacteria than Cultures seldom yielded one kind enterobacteria; E. coli Klebsiella most frequently isolated. dominated in both groups, but especially...

Previous research suggests that the World Health Organization Surgical Safety Checklist time-out reduces communication failures and medical complications supports development of better safety attitudes. also indicates different values can affect implementation interventions. To investigate actual usage checklist in practice to catalogue deviations for purpose identifying improvements. Twenty-four surgical procedures were video recorded. The was analysed quantitatively assessing compliance...

The BIRC5 gene encodes for the Survivin protein, which is a member of inhibitor apoptosis family. found in humans during fetal development, but generally not adult cells thereafter. Previous studies have shown that abundant most cancer cells, thereby making it promising target anti-cancer drugs and potential prognostic tool.To assess genetic alterations mutations as well co-expression with other genes, genomic transcriptomic data were downloaded via cBioPortal approximately 9000 samples from...

Abstract Triple-negative breast cancer (TNBC) is associated with poor prognosis and limited treatment options due to the lack of important receptors (estrogen receptor [ER], progesterone [PR], human epidermal growth factor 2 [HER2]) used for targeted therapy. However, high-throughput in vitro drug screening cell lines a powerful tool identifying effective drugs disease. Here, we determine intrinsic chemosensitivity TNBC proteasome inhibitors (PIs), thereby potentially potent 2-drug...

The in vitro attachment of 335 Proteus mirabilis strains from various human sources to urinary tract epithelial cells was measured. No significant difference adhesive capacity found between P. isolated the blood 89 patients with bacteremia, stools 36 healthy subjects and 56 diarrhea, urine 62 adults 92 children bacteriuria. High mean adhesion values were observed all groups. attached only squamous not transitional cells, whereas most Escherichia coli tested both cell types; acute...

Acute pyelonephritis in rats caused by Escherichia coli O6K13H1 produced serum and urinary antibodies against O6 K13 antigens. This was also seen after intravesical immunization with Formalin-killed bacteria. Both intraperitoneal these bacteria protected ascending tract infection induced homologous Passive transfer of urine containing both infection. By absorption experiments it shown that were especially important.

The Escherichia coli flora of infants in developed countries is dominated by one or a few strains which persist for prolonged periods time, but no longitudinal studies have been performed developing countries. To this end, we studied the rectal enterobacterial 22 home-delivered Pakistani during their first 6 months life. Three colonies were isolated and species typed on each 11 sampling occasions. E. isolates strain using electromorphic typing cytoplasmic enzymes, O serogroups determined....

LARSSON. P., and K. HENRIKSSON-LARSÉN. The use of dGPS simultaneous metabolic measurements during orienteering. Med. Sci. Sports Exerc., Vol. 33, No. 11, 2001, pp. 1919–1924. Purpose: Global Positioning System (GPS) is a satellite-based system for navigation. With this system, it possible to pinpoint subject's location on earth display the speed movement. differentiation (dGPS), accuracy GPS position can be improved. Our purpose was investigate whether would relate data with physiological...

A polyphasic taxonomic study of 14 isolates recovered from various human and veterinary clinical samples was performed. Phenotypically these shared several characteristics with members the Alcaligenaceae related genera. Random amplified polymorphic DNA fingerprinting whole-cell protein analysis suggested presence multiple genomic groups, which confirmed by DNA–DNA hybridization experiments. 16S rRNA gene sequence indicated that were to genera Pelistega , Taylorella Oligella Pigmentiphaga...

Background: Triple-negative breast cancer (TNBC) is an aggressive subtype with the most unfavorable clinical outcomes, in part due to tumor heterogeneity, treatment resistance, and relapse. The TNBC subtypes [basal-like 1 (BL1), basal-like 2 (BL2), mesenchymal (M), luminal androgen receptor (LAR)] are biologically clinically distinct entities that respond differently local systemic therapies. Therefore, we need have a better understanding of stemness relating drug-resistant populations...

We found that isolated Escherichia coli K13 antigen conjugated to bovine serum albumin, in contrast isolated, non-conjugated K13, was highly immunogenic and induced protection against acute pyelonephritis caused by E. O6K13H1 rats.

Pneumococci isolated from blood and cerebrospinal fluid 1998 to 2001 in 2 counties south-west Sweden were serotyped with the capsular reaction test. Of 836 strains, 353 (42%), 598 (72%) 789 (94%) belonged serotypes included 7- 11-valent pneumococcal conjugate vaccines 23-valent polysaccharide vaccine, respectively. The most common serotype was type 1 (119 isolates) followed descending frequency by 7F, 9V, 14, 4 12F (90–49 isolates per serotype). coverage rates of among 58 strains children...

Abstract Ovarian cancer comprises multiple subtypes (clear-cell (CCC), endometrioid (EC), high-grade serous (HGSC), low-grade (LGSC), and mucinous carcinomas (MC)) with differing molecular clinical behavior. However, robust histotype-specific biomarkers for use have yet to be identified. Here, we utilized a multi-omics approach identify novel genetic markers associated ovarian carcinoma histotypes (CCC, EC, HGSC, MC) using DNA methylation, copy number alteration RNA sequencing data 96...