A5076663151- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Microbial Natural Products and Biosynthesis
- Bacterial biofilms and quorum sensing
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and Molecular Research
- Antimicrobial Resistance in Staphylococcus
- CRISPR and Genetic Engineering
- Antimicrobial Peptides and Activities
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Computational Drug Discovery Methods
- Biochemical and Structural Characterization
- Protein Structure and Dynamics
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Click Chemistry and Applications
- Salmonella and Campylobacter epidemiology
- Microbial Community Ecology and Physiology
- Vibrio bacteria research studies
McMaster University
2016-2025
Alector (United States)
2023
University of North Carolina at Chapel Hill
2023
University of New Hampshire
2021
University of Toronto
2013
Center for Food Safety and Applied Nutrition
2012
Food and Drug Administration
2012
Dakota State University
2012
Institut de Biologie Moléculaire et Cellulaire
2011
Indian Institute of Technology Roorkee
2008
Abstract The Comprehensive Antibiotic Resistance Database (CARD; card.mcmaster.ca) combines the Ontology (ARO) with curated AMR gene (ARG) sequences and resistance-conferring mutations to provide an informatics framework for annotation interpretation of resistomes. As version 3.2.4, CARD encompasses 6627 ontology terms, 5010 reference sequences, 1933 mutations, 3004 publications, 5057 detection models that can be used by accompanying Gene Identifier (RGI) software annotate genomic or...
Plasmid-borne colistin resistance mediated by mcr-1 may contribute to the dissemination of pan-resistant Gram-negative bacteria. Here, we show that confers colistin-induced lysis and bacterial cell death, but provides minimal protection from ability disrupt outer membrane. Indeed, for colistin-resistant strains Enterobacteriaceae expressing plasmid-borne mcr-1, clinically relevant concentrations potentiate action antibiotics that, themselves, are not active against The result is several...
SARS-CoV-2 is associated with broad tissue tropism, a characteristic often determined by the availability of entry receptors on host cells. Here, we show that TMEM106B, lysosomal transmembrane protein, can serve as an alternative receptor for into angiotensin-converting enzyme 2 (ACE2)-negative Spike substitution E484D increased TMEM106B binding, thereby enhancing TMEM106B-mediated entry. TMEM106B-specific monoclonal antibodies blocked infection, demonstrating role in viral Using X-ray...
The Escherichia coli gene murZ was recently shown to encode UDP-N-acetylglucosamine enolpyruvyl transferase, which catalyzes the first committed step of peptidoglycan biosynthesis (J. L. Marquardt, D. A. Siegele, R. Kolter, and C. T. Walsh, J. Bacteriol. 174:5748-5752, 1992). map position (69.3 min) differed from that determined for murA (90 min), a had been previously proposed same activity (P.S. Venkateswaran H. Wu, 110:935-944, 1972). Here we describe construction chromosomal deletion...
Rising drug resistance is limiting treatment options for infections by methicillin-resistant Staphylococcus aureus (MRSA). Herein we provide new evidence that wall teichoic acid (WTA) biogenesis a remarkable antibacterial target with the capacity to destabilize cooperative action of penicillin-binding proteins (PBPs) underlie β-lactam in MRSA. Deletion gene tarO, encoding first step WTA synthesis, resulted restoration sensitivity MRSA unique profile antibiotics known selectivity penicillin...
Article21 June 2011Open Access Cross-species discovery of syncretic drug combinations that potentiate the antifungal fluconazole Michaela Spitzer Wellcome Trust Centre for Cell Biology, School Biological Sciences, University Edinburgh, UK Search more papers by this author Emma Griffiths Michael G. DeGroote Institute Infectious Disease Research and Department Biochemistry Biomedical McMaster University, Hamilton, Ontario, Canada Smith Laboratories, British Columbia, Vancouver, Kim M Blakely...
ABSTRACT An extensive study of teichoic acid biosynthesis in the model organism Bacillus subtilis has established polymers as essential components gram-positive cell wall. However, similar studies pertaining to therapeutically relevant organisms, such Staphylococcus aureus , are scarce. In this we have carried out a meticulous examination dispensability biosynthetic enzymes S. . By use an allelic replacement methodology, examined all facets assembly, including intracellular polymer...
An extensive literature has established that the synthesis of wall teichoic acid in Bacillus subtilis is essential for cell viability. Paradoxically, we have recently shown biogenesis dispensable Staphylococcus aureus (M. A. D'Elia, M. P. Pereira, Y. S. Chung, W. Zhao, Chau, T. J. Kenney, C. Sulavik, Black, and E. D. Brown, Bacteriol. 188:4183-4189, 2006). A complex pattern gene dispensability was seen where first (tarO) later acting genes showed an indispensable phenotype. Here show, time,...
Large-scale proteomic analyses in Escherichia coli have documented the composition and physical relationships of multiprotein complexes, but not their functional organization into biological pathways processes. Conversely, genetic interaction (GI) screens can provide insights role(s) individual gene higher order associations. Combining information from both approaches should elucidate how complexes intersect functionally at a systems level. However, such integrative analysis has been...
The antibacterial properties of sodium bicarbonate have been known for years, yet the molecular understanding its mechanism action is still lacking. Utilizing chemical–chemical combinations, we first explored effect on activity conventional antibiotics to infer mechanism. Remarkably, 8 classes differed in presence this ubiquitous buffer. These interactions and a study revealed that, at physiological concentrations, selective dissipater pH gradient proton motive force across cytoplasmic...