A5013655400- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Histone Deacetylase Inhibitors Research
- Acute Lymphoblastic Leukemia research
- Retinoids in leukemia and cellular processes
- CAR-T cell therapy research
- Pluripotent Stem Cells Research
- Hematopoietic Stem Cell Transplantation
- Multiple Myeloma Research and Treatments
- Protein Degradation and Inhibitors
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Hemoglobinopathies and Related Disorders
- Cytokine Signaling Pathways and Interactions
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Biomedical Ethics and Regulation
- Neuroblastoma Research and Treatments
- Zebrafish Biomedical Research Applications
Josep Carreras Leukaemia Research Institute
2014-2023
Leuka
2022
Fundación Josep Carreras Contra la Leucemia
2015-2019
McMaster University
2008-2018
Universitat de Barcelona
2015-2016
Leukemia Research Foundation
2015
Ontario Institute for Cancer Research
2010
Consejo Superior de Investigaciones Científicas
2005-2009
Universidad Autónoma de Madrid
2005-2009
Centro de Biología Molecular Severo Ochoa
2005-2009
A long-standing paradox in the study of T cell antigen recognition is that high specificity–low affinity receptor (TCR)–major histocompatibility complex peptide (MHCp) interaction. The existence multivalent TCRs could resolve this because they can simultaneously improve avidity observed for monovalent interactions and allow cooperative effects. We have studied stoichiometry TCR by Blue Native–polyacrylamide gel electrophoresis found exists as a mixture (αβγεδεζζ) complexes with two or more...
The CD3epsilon subunit of the T cell receptor (TCR) complex undergoes a conformational change upon ligand binding that is thought to be important for activation cells. To study this process, we built molecular dynamics model transmission within ectodomains CD3. showed CD3 dimers underwent stiffening effect was funneled base subunit. Mutation two relevant amino acid residues blocked and differentiation Furthermore, inhibition occurred even in presence excess endogenous subunits. These results...
// Marina Díaz-Beyá 1, 2 , Salut Brunet 2, 3 Josep Nomdedéu 4 Marta Pratcorona Anna Cordeiro 5 David Gallardo 6 Lourdes Escoda 7 Mar Tormo 8 Inmaculada Heras 9 Maria Ribera 10 Rafael Duarte 11 María Paz Queipo de Llano 12 Joan Bargay 13 Antonia Sampol 14 Meritxell Nomdedeu 1 Ruth M. Risueño Montserrat Hoyos Jorge Sierra Mariano Monzo Alfons Navarro 5, * Jordi Esteve 15, on behalf of the Cooperative AML group CETLAM Hematology Department, Hospital Clinic,...
Purpose of review Hematopoietic homeostasis depends on appropriate self-renewal and differentiation capacity hematopoietic stem cells. The characterization the key extracellular signals that integrate with intracellular molecular machinery to regulate cells fate choice is crucial move toward cell clinical application. Recent findings Several factors have been described as positive negative regulators differentiation. Most cytokines studied promote either survival or both in ex vivo, whereas...
Germ line predisposition in acute myeloid leukemia (AML) has gained attention recent years because of a nonnegligible frequency and an impact on management patients their relatives. Risk alleles for AML development may be present without clinical suspicion hereditary hematologic malignancy syndrome. In this study we investigated the presence germ variants (GVs) 288 genes related to cancer 47 with available paired, tumor-normal material, namely bone marrow stroma cells (n = 29), postremission...
Abstract Hematopoietic stem cells (HSCs) can regenerate the entire hematopoietic system in vivo, providing most relevant criteria to measure candidate HSCs derived from human embryonic cell (hESC) or induced pluripotent (hiPSC) sources. Here we show that, unlike primitive hESCs, phenotypically identical hiPSC are more permissive graft bone marrow of xenotransplantation recipients. Despite establishment graft, hiPSC-derived fail demonstrate differentiation vivo. However, once removed...
// Daniel Moreno-Martínez 1 , Meritxell Nomdedeu 1,2,3 María Carmen Lara-Castillo Amaia Etxabe Marta Pratcorona 2,3 Niccolò Tesi Marina Díaz-Beyá Rozman 4 Emili Montserrat Álvaro Urbano-Ispizua Jordi Esteve Ruth M. Risueño Josep Carreras Leukaemia Research Institute. Barcelona, Spain 2 Department of Hematology, Hospital Clínic de Barcelona. 3 Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hematopathology, Correspondence: Risueño, email: Keywords : Acute myeloid leukemia, leukemic...
How the T cell antigen receptor (TCR) discriminates between molecularly related peptide/Major Histocompatibility Complex (pMHC) ligands and converts this information into different possible signaling outcomes is still not understood. One current model proposes that strong pMHC ligands, but weak ones, induce a conformational change in TCR. Evidence supporting comes from pull-down assay detects ligand-induced binding of TCR to N-terminal SH3 domain adapter protein Nck, also studies with...
BackgroundDespite great efforts to identify druggable molecular targets for AML, there remains an unmet need more effective therapies.MethodsAn in silico screening was performed using Connectivity Maps FDA-approved drugs that may revert early leukaemic transformation gene signature. Hit compounds were validated AML cell lines. Cytotoxic effects assessed both primary patient samples and healthy donor blood cells. Xenotransplantation assays undertaken determine the effect on engraftment of hit...
T cell antigen receptor (TCR) triggering determines the fate of immature thymocytes. The affinity TCR for its endogenous peptide/MHC ligands serves as a signal positive or negative selection through mechanisms that are still little understood. We have used conformation-specific antibody to demonstrate recognition lead induces conformational change in situ. In contrast, this is elicited only small percentage thymocytes during selection. Using TUNEL assay, we strongly linked activation...
Treatment for acute myeloid leukemia (AML) has not significantly changed in the last decades and new therapeutic approaches are needed to achieve prolonged survival rates. Leukemia stem cells (LSC) responsible initiation maintenance of AML due their stem-cell properties. Differentiation therapies aim abrogate self-renewal capacity diminish blast lifespan.An silico screening was designed search FDA-approved small molecules that potentially induce differentiation cells. Bromocriptine...
Abstract Acute myeloid leukemia (AML) is a heterogeneous disease whose prognosis mainly related to the biological risk conferred by cytogenetics and molecular profiling. In elderly patients (⩾60 years) with normal karyotype AML miR-3151 have been identified as prognostic factor. However, value has not examined in younger patients. present work, we studied alone combination BAALC , its host gene, cohort of 181 intermediate-risk (IR-AML) Patients higher expression had shorter overall survival...
// Josep Maria Cornet-Masana 1 , Daniel Moreno-Martínez María Carmen Lara-Castillo Meritxell Nomdedeu 1,2 Amaia Etxabe Niccolò Tesi Marta Pratcorona Jordi Esteve and Ruth M. Risueño Carreras Leukaemia Research Institute, Barcelona, Spain 2 Department of Hematology, Hospital Clínic, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Correspondence to: Risueño, email: Keywords : AML, emetine, anti-leukemia drug Received...
Summary The search for disease-associated T helper 2 (Th2) Leishmania antigens and the induction of a Th1 immune response to them using defined vaccination protocols is potential strategy induce protection against infection. infantum LiP2a LiP2b acidic ribosomal protein (P proteins) have been described as prominent during human canine visceral leishmaniasis. In this study we demonstrate that BALB/c mice infected with major develop Th2-like humoral proteins same induced in when parasite P are...
We have previously shown that the surface alphabeta T cell antigen receptor (TCR).CD3 complex borne by human CD4(+) and CD8(+) lymphocytes can be distinguished using mAbs. Using two unrelated sets of antibodies, we now extended this finding to alphabetaTCR.CD3 seven additional mammalian species (six non-human primates mouse). also produced data supporting differential glycosylation main subsets is involved in observed TCR.CD3 antibody-binding differences humans. First, show lectin binding...
Myelodysplastic syndromes (MDS) and chronic myelomonocytic leukemia (CMML) are myeloid clonal neoplasms. To date, the only potentially curative therapy for these disorders remains allogeneic hematopoietic progenitor cell transplantation (HCT), although patient eligibility is limited due to high morbimortality associated with this procedure coupled advanced age of most patients. Dopamine receptors (DRs) serotonin type 1 (HTR1s) were identified as cancer stem therapeutic targets in acute...
The multiple myeloma (MM) landscape has changed in the last few years, but most patients eventually relapse because current treatment modalities do not target clonogenic stem cells, which are drug-resistant and can self-renew. We hypothesized that side population (SP) cells represent and, searching for new strategies, analyzed anti-myeloma activity of natural killer (NK) against cells. Activated expanded NK (NKAE) products were obtained by co-culturing from MM with K562-mb15-41BBL cell line...