A5067684103- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Kruppel-like factors research
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Eosinophilic Disorders and Syndromes
- Electromagnetic Fields and Biological Effects
- Adenosine and Purinergic Signaling
- Hematopoietic Stem Cell Transplantation
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Microbial Inactivation Methods
- Epigenetics and DNA Methylation
- Immune cells in cancer
- COVID-19 Clinical Research Studies
- Chemokine receptors and signaling
- RNA Interference and Gene Delivery
- Platelet Disorders and Treatments
- Tissue Engineering and Regenerative Medicine
- Endoplasmic Reticulum Stress and Disease
- Immunotherapy and Immune Responses
- Computational Drug Discovery Methods
- Hemoglobinopathies and Related Disorders
IGEA Clinical Biophysics (Italy)
2020-2024
Interventi Geo Ambientali (Italy)
2022-2024
University of Modena and Reggio Emilia
2010-2019
Ferrari (Italy)
2013-2019
Cancer Research Institute
2008
McMaster University
2008
Abstract This study was aimed at the characterization of a gene expression signature pluripotent hematopoietic CD34+ stem cell in idiopathic myelofibrosis (IM), which would eventually provide novel pathogenetic insights and/or diagnostic/prognostic information. Aberrantly regulated genes were revealed by transcriptome comparative microarray analysis normal and IM cells; selected also assayed granulocytes. One-hundred seventy four differentially expressed identified part validated...
The transcription factor MYB has a key role in hematopoietic progenitor cells (HPCs) lineage choice, by enhancing erythropoiesis at the expense of megakaryopoiesis. We previously demonstrated that controls erythroid versus megakaryocyte decision transactivating KLF1 and LMO2 expression. To further unravel molecular mechanisms through which affects fate decision, we performed integrative analysis miRNA mRNA changes MYB-silenced human primary CD34+ HPCs. Among miRNAs with highest number...
The development of Imatinib mesylate (IM), which targets the oncogenic BCR-ABL fusion protein, has greatly improved outcome Chronic Myeloid Leukemia (CML) patients. However, BCR-ABL-positive progenitors can be detected in CML patients complete cytogenetic response. Several evidence suggests that stem cells are intrinsically resistant to Tyrosine Kinase Inhibitors (TKI), and therefore they represent most likely candidate responsible for disease relapse.In this work, we investigated microRNA...
Abstract Somatic mutations of calreticulin (CALR) have been described in approximately 60–80% JAK2 and MPL unmutated Essential Thrombocythemia Primary Myelofibrosis patients. CALR is an endoplasmic reticulum (ER) chaperone responsible for proper protein folding calcium retention. Recent data demonstrated that the TPO receptor (MPL) essential development mutant-driven Myeloproliferative Neoplasms (MPNs). However, precise mechanism action mutants haven’t fully unraveled. In this study, we...
BACKGROUND In the first studies of electrochemotherapy (ECT), small cutaneous metastases were treated and only mild or moderate pain was observed; therefore, not considered a significant issue. As procedure began to be applied larger metastases, reported more frequently. For that reason, reduction both muscle contractions have been investigated over years. AIM To present an overview different protocols described in literature aim reduce caused by electroporation (EP) effect ECT irreversible...
In bone tissue engineering research, bioreactors designed for replicating the main features of complex native environment represent powerful investigation tools. Moreover, when equipped with automation, their use allows reducing user intervention and dependence, increasing reproducibility overall quality culture process. this study, an automated uni-/bi-directional perfusion bioreactor combinable pulsed electromagnetic field (PEMF) stimulation culturing 3D models is proposed. A user-friendly...
Pulsed electromagnetic fields (PEMFs) are clinically used with beneficial effects in the treatment of bone fracture healing. This is due to PEMF ability favor osteogenic differentiation mesenchymal stem cells (MSCs). Previous studies suggest that PEMFs enhance activity morphogenetic protein-2 (BMP2) which various therapeutic interventions. study investigated molecular events associated synergistic and BMP2 on differentiation. To this aim, human MSCs (hMSCs) were exposed (75 Hz, 1.5 mT)...
Purpose of review Hematopoietic homeostasis depends on appropriate self-renewal and differentiation capacity hematopoietic stem cells. The characterization the key extracellular signals that integrate with intracellular molecular machinery to regulate cells fate choice is crucial move toward cell clinical application. Recent findings Several factors have been described as positive negative regulators differentiation. Most cytokines studied promote either survival or both in ex vivo, whereas...
Abstract Polycythemia vera (PV) and essential thrombocythemia (ET) are Philadelphia-negative myeloproliferative neoplasms (MPNs) characterized by erythrocytosis thrombocytosis, respectively. Approximately 95% of PV 50–70% ET patients harbor the V617F mutation in exon 14 JAK2 gene, while about 20–30% carry CALRins5 or CALRdel52 mutations. These CALR-mutated subjects show higher platelet count lower thrombotic risk compared to JAK2-mutated patients. Here, we showed that JAK2V617F-positive...
Primary myelofibrosis (PMF) is a myeloproliferative neoplasm characterized by hyperplastic megakaryopoiesis and myelofibrosis. We recently described the upregulation of MAF (v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog) in PMF CD34+ hematopoietic progenitor cells (HPCs) compared to healthy donor. Here we demonstrated that also upregulated with essential thrombocytemia (ET) polycytemia vera (PV) HPCs. overexpression knockdown experiments shed some light into role pathogenesis,...
The gene expression profile of CD34(-) hematopoietic stem cells (HSCs) and the correlations with their biological properties are still poorly understood. To address this issue, we used DNA microarray technology to compare profiles different peripheral blood hemopoietic stem/progenitor cell subsets, lineage-negative (Lin(-)) CD34(-), Lin(-)CD34(+), Lin(+)CD34(+) cells. analysis categories differentially expressed shows that CD34 is associated cycle entry metabolic activation, such as DNA,...
Abstract CD34 is a transmembrane protein that strongly expressed on hematopoietic stem/progenitor cells (HSCs); despite its importance as marker of HSCs, function still poorly understood, although role in cell adhesion has been demonstrated. To characterize the antigen human we examined, by both inhibition and overexpression, regulation HSC lineage differentiation. Our results demonstrate silencing enhances granulocyte megakaryocyte differentiation reduces erythroid maturation. In agreement...
microRNAs are key regulators of gene expression that control stem cell fate by posttranscriptional downregulation hundreds target genes through seed pairing in their 3′ untranslated region. In fact, miRNAs tightly regulate fundamental processes, like self-renewal, proliferation, and differentiation; therefore, miRNA deregulation may contribute to the development solid tumors hematological malignancies. miR-382-5p has been found be upregulated patients with myeloid neoplasms, but its role...
Primary myelofibrosis (PMF) is a myeloproliferative neoplasm characterized by an excessive production of pro-inflammatory cytokines resulting in chronic inflammation and genomic instability. Besides the driver mutations JAK2, MPL, CALR genes, deregulation miRNA expression may also contribute to pathogenesis PMF. To this end, we recently reported upregulation miR-382-5p PMF CD34+ cells. In order unveil mechanistic details role PMF, performed gene profiling cells overexpressing miR-382-5p....
Calreticulin (CALR) is a chaperone protein that localizes primarily to the endoplasmic reticulum (ER) lumen where it responsible for control of proper folding neo-synthesized glycoproteins and retention calcium. Recently, mutations affecting exon 9 CALR gene have been described in approximately 40% patients with myeloproliferative neoplasms (MPNs). Although role mutated development MPNs has begun be clarified, there are still no data available on function wild-type (WT) during physiological...