A5059343991- Hepatitis C virus research
- Hepatitis B Virus Studies
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Liver Disease Diagnosis and Treatment
- HIV Research and Treatment
- Immune Cell Function and Interaction
- Hepatitis Viruses Studies and Epidemiology
- Systemic Lupus Erythematosus Research
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- CAR-T cell therapy research
- HIV/AIDS drug development and treatment
- Glycosylation and Glycoproteins Research
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Protein purification and stability
- Viral gastroenteritis research and epidemiology
- Diabetes and associated disorders
- Viral Infections and Immunology Research
- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- T-cell and B-cell Immunology
- Mosquito-borne diseases and control
- Liver Diseases and Immunity
Karolinska Institutet
2015-2025
Karolinska University Hospital
2013-2024
Svenska Örtmedicinska Institute
2023
Leipzig University
2022
Czech Academy of Sciences, Institute of Microbiology
2018
Stockholm University
2011
Janssen (Belgium)
2011
Hospices Civils de Lyon
2011
GlaxoSmithKline (United States)
2011
University of Cologne
2009
A unique characteristic of the hepatitis B virus is production a secreted form (precore or HBeAg) structural nucleocapsid (core HBcAg). By using T cell receptor (TCR) transgenic (Tg) and TCR x HBc/HBeAg double- triple-Tg pairs, we demonstrate that HBeAg elicits tolerance, whereas HBcAg nontolerogenic in this system. In fact, HBc double-Tg mice spontaneously seroconvert to IgG anti-HBc positivity at an early age. However, presence serum HBe prevents seroconversion. mediates its...
ABSTRACT The function of the hepatitis B virus (HBV) precore or HBeAg is largely unknown because it not required for viral assembly, infection, replication. However, does appear to play a role in persistence. It has been suggested that may promote HBV chronicity by functioning as an immunoregulatory protein. As model chronic exposure and examine tolerogenic potential core (HBcAg) proteins, HBc/HBeAg-transgenic (Tg) mice crossed with T cell receptor (TCR)-Tg expressing receptors HBc/HBeAgs...
Abstract Patients with I g E antibodies against the carbohydrate epitope galactose‐α‐1,3‐galactose (α‐ G al) have reported severe allergic reactions after consumption of red meat. Investigations revealed associations between to α‐ al and tick bites. We provide first direct evidence that is present within ticks thus potentially explaining relationship exposure sensitization al, development meat allergy as a secondary phenomena. Serum from S wedish patients delayed was included in study. A...
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged in late and has since become a global pandemic. Pathogen-specific Abs are typically major predictor of protective immunity, yet human B cell Ab responses during COVID-19 not fully understood. In this study, we analyzed Ab-secreting 20 hospitalized patients. The patients exhibited typical symptoms presented with reduced lymphocyte numbers increased T activation. Importantly,...
Mpox represents a persistent health concern with varying disease severity. Reinfections mpox virus (MPXV) are rare, possibly indicating effective memory responses to MPXV or related poxviruses, notably vaccinia (VACV) from smallpox vaccination. We assessed cross-reactive and virus-specific CD4
Abstract The mechanisms by which in vivo electroporation (EP) improves the potency of i.m. DNA vaccination were characterized using hepatitis C virus nonstructural (NS) 3/4A gene. Following a standard injection with or without EP, plasmid levels peaked immediately at site and decreased 4 logs first week. In EP did not promote persistence and, depending on dose, was cleared almost after 60 days. imaging immunohistochemistry revealed that protein expression restricted to despite detection...
Hepatocellular carcinoma (HCC) cells often have hepatitis B virus (HBV)-DNA integration and can be targeted by HBV-specific T cells. The use of viral vectors to introduce exogenous T-cell receptors (TCR) on redirect their specificity is complex expensive implement in clinical trials. Moreover, it raises safety concerns related insertional mutagenesis potential toxicity long-lived patients with persistent infection. To develop a more practical safer approach cell therapy HCC, we used...
Clearance of infections caused by the hepatitis C virus (HCV) correlates with HCV-specific T cell function. We therefore evaluated therapeutic vaccination in 12 patients chronic HCV infection. Eight also underwent a subsequent standard-of-care (SOC) therapy pegylated interferon (IFN) and ribavirin. The phase I/IIa clinical trial was performed treatment naive genotype 1 patients, receiving four monthly vaccinations deltoid muscles 167, 500, or 1,500 μg codon-optimized nonstructural (NS)...
Crimean-Congo hemorrhagic fever (CCHF), caused by virus (CCHFV), is on the World Health Organizations' list of prioritized diseases and pathogens. With global distribution, high fatality rate, no approved vaccine or effective treatment, CCHF constitutes a threat against health. In current study, we demonstrate that vaccination with nucleoside-modified mRNA-lipid nanoparticles (mRNA-LNP), encoding for CCHFV nucleoprotein (N) glycoproteins (GcGn) protect IFNAR-/- mice lethal infection....
Abstract Objectives Humoral and cellular immunity to SARS‐CoV‐2 following COVID‐19 will likely contribute protection from reinfection or severe disease. It is therefore important characterise the initiation persistence of adaptive amidst ongoing pandemic. Methods Here, we conducted a longitudinal study on hospitalised moderate patients acute phase disease into convalescence at 5 9 months post‐symptom onset. Utilising flow cytometry, serological assays as well B cell T FluoroSpot assays,...
Background. Hepatitis B vaccine has been shown to be highly efficient in preventing hepatitis B. However, 5%–10% of individuals fail develop protective levels (≥10 mIU/mL) antibodies surface antigen (anti-HBs) and are considered nonresponders.
Previous studies demonstrated that the primary APCs for hepatitis B core Ag (HBcAg) were cells and not dendritic (DC). We now report splenic B1a B1b more efficiently present soluble HBcAg to naive CD4(+) T than B2 cells. This was by direct HBcAg-biotin-binding HBcAg-specific cell activation in vitro cultures of resting subpopulations. The inability DC function as exogenous relates lack uptake HBcAg, processing or presentation, because HBcAg/anti-HBc immune complexes can be presented DC....
The hepatitis C virus (HCV) is a worldwide major cause of chronic liver disease with high tendency to establish persistent infection. To permit replication viral genomes through the cellular translation machinery without affecting host cell viability, viruses must have developed mechanisms control cascades required for sufficient replication, on one hand, and adapt requirements other hand. present study aimed further elucidate by which HCV targets growth factor signaling their implications...
Crimean-Congo hemorrhagic fever virus (CCHFV) is widely distributed throughout Africa, the Middle East, Southern Asia, and Eastern Europe. Spread by Hyalomma ticks or contact with infected animals, CCHF begins non-specifically but can rapidly progress to severe, sometimes fatal, disease. Due non-specific early symptoms often unrecognized infections, patients present healthcare systems exhibiting later stages of disease, when treatment limited supportive care. Consequently, simple vaccines...
Background Tumour-infiltrating T cells can mediate both antitumour immunity and promote tumour progression by creating an immunosuppressive environment. This dual role is especially relevant in hepatocellular carcinoma (HCC), characterised a unique microenvironment limited success with current immunotherapy. Objective We evaluated cell responses patients advanced HCC analysing tumours, liver flushes liver-draining lymph nodes, to understand whether reactive populations could be identified...
The hepatitis B virus (HBV) core antigen (HBcAg) has a unique ability to bind high frequency of naive human and murine cells. role HBcAg-binding cells in the immunogenicity HBcAg is not clear. properties were characterized using particles with mutated spike region (residues 76–85) sequences. Deletion residues 76–85 (HBcΔ76–85) destroyed cell binding, whereas deletion 79–85 did not. an Ile instead natural Ala at position 80 cells, reversion Ile80→Ala restored binding. Destroying cell-binding...
A complementarity-determining region (CDR) of the mouse monoclonal antibody (mAb) F58 was constructed with specificity to a neutralization-inducing human immunodeficiency virus type 1 (HIV-1). The mAb has its major reactivity amino acid sequence I--GPGRA in V3 viral envelope region. All CDRs including several framework acids were synthesized from deduced by cloning and sequencing heavy- light-chain variable domains. Peptides derived third heavy-chain domain (CDR-H3) alone or combination...
Abstract The complete amino acid (aa) sequence of the hepatitis B virus (HBV) core protein (HBcAg), ayw subtype, was synthesized as decapeptides with five overlapping aas. peptides were tested for reactivity monoclonal antibodies (mAbs) to HBcAg (35/312, 37/275, and 7/275). All mAbs specifically inhibited human anti‐HBc by cross competition in assays anti‐HBe. mAb 35/312 recognised a peptide covering residues 76–85 sequence. other two did not react any linear peptide, suggesting...