A5035188920- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Systemic Lupus Erythematosus Research
- Protein Tyrosine Phosphatases
- interferon and immune responses
- Influenza Virus Research Studies
- Liver Diseases and Immunity
- Cytokine Signaling Pathways and Interactions
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- Liver physiology and pathology
- Immunotherapy and Immune Responses
- Plant-Microbe Interactions and Immunity
- Transgenic Plants and Applications
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS drug development and treatment
- Animal Virus Infections Studies
- Liver Disease and Transplantation
- RNA Interference and Gene Delivery
- Protein purification and stability
- Pancreatic function and diabetes
- Immunodeficiency and Autoimmune Disorders
Czech Academy of Sciences, Institute of Microbiology
2018
Karolinska Institutet
2009-2017
Karolinska University Hospital
2009-2013
Janssen (Belgium)
2011
Heinrich Heine University Düsseldorf
2005-2010
Düsseldorf University Hospital
2008-2009
University Hospital Bonn
2009
Maulana Azad Medical College
2009
MSB Medical School Berlin
2005
University of Freiburg
2005
The potential of some proinflammatory mediators to inhibit gp130-dependent STAT3 activation by enhancing suppressor cytokine signaling (SOCS) 3 expression represents an important molecular mechanism admitting the modulation cellular response toward gp130-mediated signals. Thus, it is necessary understand mechanisms involved in regulation SOCS3 mediators. In this study, we investigate initiated TNF-alpha. contrast IL-6, TNF-alpha increases stabilizing mRNA. Activation MAPK kinase 6...
The hepatitis C virus (HCV) is a worldwide major cause of chronic liver disease with high tendency to establish persistent infection. To permit replication viral genomes through the cellular translation machinery without affecting host cell viability, viruses must have developed mechanisms control cascades required for sufficient replication, on one hand, and adapt requirements other hand. present study aimed further elucidate by which HCV targets growth factor signaling their implications...
Tumor necrosis factor α (TNFα) has been implicated in a variety of inflammatory diseases, and anti-TNFα shown to improve therapy when added standard care chronic hepatitis C virus (HCV) infection. In addition, patients with HCV have increased serum levels TNFα the macrophage-attracting chemokine (C-C motif) ligand 2 (CCL2). A mouse model hepatic nonstructural (NS) 3/4A protein expression mimics human infection through reduced response double-stranded RNA cleavage T cell tyrosine phosphatase....
Virus-specific CTL with high levels of functional avidity have been associated viral clearance in hepatitis C virus (HCV) infection and enhanced protective immunity. In chronic HCV infection, lack antiviral is frequently observed. this study, we aim to investigate novel TCRs that differ Ag specificity. This involved isolating new HCV-specific murine recognize a conserved HLA-A2-restricted epitope within the nonstructural protein (NS) 5A comparing them recognizing another target NS3 protein....
The hepatitis C virus (HCV)-specific T cell response in patients with chronic HCV is dysfunctional. In this study, we aimed at restoring immunological function through therapeutic vaccination a transgenic mouse model impaired HCV-specific responses due to persistent presence of hepatic nonstructural (NS)3/4A Ags. cells have an actively maintained dysfunction reflected reduced frequency, cytokine production, and effector vivo, which can be partially restored by blocking regulatory or...
Objectives Curing of hepatitis C virus (HCV) infection primarily aims to prevent severe liver complications. Our objectives were investigate the long-term presence and impact occult HCV (OCI) study outcomes in terms disease after virological cure. Patients methods A total 97 patients with achieved sustained response (SVR) during 1990–2005 followed either by a clinical follow-up (FU) visit blood sampling elastography ( n =54) or through national registries for =43). To diagnose OCI among SVR,...
Abstract Ribavirin has proven to be a key component of hepatitis C therapies both involving IFNs and new direct-acting antivirals. The virus–mediated interference with intrahepatic immunity by cleavage mitochondrial antiviral signaling protein (MAVS) T cell tyrosine phosphatase (TCPTP) suggests an avenue for compounds that may counteract these effects. We therefore studied the effects ribavirin, or without inhibition nonstructural (NS)3/4A protease, on immunity. wild-type NS3/4A-transgenic...
<h3>Background</h3> The non-structural (NS) 3/4A protease/helicase of the hepatitis C virus is known to modulate signalling pathways in infected hepatocyte by cleaving CARD adaptor inducing IFNβ (Cardif), T-cell protein tyrosine phosphatase (TC-PTP) and TIR domain-containing (TRIF), but effects NS3/4A vivo still remain unclear. <h3>Aim</h3> To investigate influence on intracellular intercellular analysing intrahepatic inflammatory response naïve, lipopolysaccharide (LPS)/d-galactosamine...
Background A sustained viral response (SVR) after interferon-based therapy of chronic hepatitis C virus (HCV) infection is regarded to represent a cure. Previous studies have used different markers clarify whether an SVR truly represents cure, but no study has combined clinical work-up with highly sensitive HCV RNA detection, and the determination immune responses. Aim To determine clinical, histological, virological immunological 5–20 years SVR. Methods In 54 patients, liver biochemistry,...
HCV infection typically induces liver injury and inflammation, which appears to be responsible for the associated fibrogenesis. To date, mechanism underlying different rates of disease progression remains unclear. The aim study is understand possible role non-structural (NS) 3/4A protein in fibrosis progression. We used NS3/4A-expressing transgenic mice (NS3/4A-Tg) accomplish goals study. Different stages were induced wild-type NS3/4A-Tg by single carbon tetrachloride (acute) or multiple...
Vertical transmission of hepatitis C virus (HCV) infection is uncommon and occurs in approximately 5% births from HCV-infected mothers. The reason for the low rate unclear. We aimed to investigate whether there evidence HCV exposure also noninfected children born mothers by presence a detectable immune response.Serum peripheral blood mononuclear cells 9 vertically infected children, 32 uninfected mothers, 15 chronically were analyzed. HCV-RNA-negative adults used as controls. HCV-specific T...
Single genetic nucleotide polymorphism (rs12979860) near the gene for interleukin 28B (IL28B) is known to be of importance frequency spontaneous clearance and treatment outcome in interferon-based therapies patients with hepatitis C virus (HCV) infection. The aim present study was investigate whether IL28B children and/or their mothers plays a role vertical transmission HCV (HCV-VT).Plasma samples from 59 infected women, 76 uninfected born mothers, 47 vertically transmitted infection, were...
The study was designed to evaluate the ability of calcium sulfate based NanoZolid® drug delivery technology locally release epidermal growth factor (EGF) protein while maintaining its biological activity. NanoZolid-formulated EGF labelled with a near infrared dye (EGF-NIR) depots or EGF-NIR dissolved in PBS were injected subcutaneously into mice bearing receptor (EGFR) positive human A549 lung cancer tumors inoculated subcutaneously. and biodistribution investigated vivo longitudinally up 96...