A5005186173- Hepatitis B Virus Studies
- Hepatitis C virus research
- Immunotherapy and Immune Responses
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Liver Disease Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Alcohol Consumption and Health Effects
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Animal Virus Infections Studies
- HIV Research and Treatment
- Viral Infections and Outbreaks Research
- Cytomegalovirus and herpesvirus research
- Heme Oxygenase-1 and Carbon Monoxide
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Cell Adhesion Molecules Research
- Herpesvirus Infections and Treatments
- Nanoparticle-Based Drug Delivery
- T-cell and B-cell Immunology
- Pneumocystis jirovecii pneumonia detection and treatment
- Hepatitis Viruses Studies and Epidemiology
- Glycosylation and Glycoproteins Research
Latvian Biomedical Research and Study Centre
2011-2024
University of Latvia
1994-2015
Latvian Maritime Academy
1992-1993
Assembly and disassembly of viral capsids are essential steps in the life cycle. Studies on their kinetics mostly performed vitro, allowing application biochemical, biophysical visualizing techniques. In vivo poorly understood transferability vitro models to cellular environment remains speculative. We analyzed capsid hepatitis B virus digitonin-permeabilized cells which support nuclear entry subsequent genome release. Using gradient centrifugation, size exclusion chromatography immune...
ABSTRACT A multivalent vaccine candidate against hepatitis B virus (HBV) and C (HCV) infections was constructed on the basis of HBV core (HBc) virus-like particles (VLPs) as carriers. Chimeric VLPs that carried a virus-neutralizing pre-S1 epitope corresponding to amino acids (aa) 20 47 in major immunodominant region (MIR) highly conserved N-terminal HCV aa 1 60 at terminus truncated HBcΔ protein (N-terminal 144 full-length HBc) were produced Escherichia coli cells examined for their...
Hepatitis C virus (HCV) infection is accompanied by the induction of oxidative stress, mediated several proteins, most prominent being nucleocapsid protein (HCV core). Here, using truncated forms HCV core, we have delineated mechanisms which it induces stress. The N-terminal 36 amino acids core induced TGFβ1-dependent expression nicotinamide adenine dinucleotide phosphate (NADPH) oxidases 1 and 4, both independently contributed to production reactive oxygen species (ROS). same fragment also...
The hepatitis B virus (HBV) core antigen (HBcAg) has a unique ability to bind high frequency of naive human and murine cells. role HBcAg-binding cells in the immunogenicity HBcAg is not clear. properties were characterized using particles with mutated spike region (residues 76–85) sequences. Deletion residues 76–85 (HBcΔ76–85) destroyed cell binding, whereas deletion 79–85 did not. an Ile instead natural Ala at position 80 cells, reversion Ile80→Ala restored binding. Destroying cell-binding...
Abstract Hospital‐acquired hepatitis B (HBV) and C virus (HCV) infections continue to occur despite increased awareness of this problem among the medical community. One hundred six patients were infected in a haematology oncology ward for children, over time period 1996 2000. Serum samples from 45 such 3 personnel used nucleic acid amplification. HBV core, as well HCV core hypervariable region 1 (HVR1) nucleotide sequences, analysed by phylogenetic tree analysis, order characterise...
An efficient pBR327- and Ptrp-based E. coli expression system was used to generate a large-scale library of virus like particles (VLP) formed by recombinant hepatitis B (HBV) core (HBc) protein derivatives. To construct the library, gene HBc genotype D/subtype ayw2 gradually truncated from 3`-end twenty-two variants (with truncation up 139 aa) were expressed at high levels. The proteins purified salt precipitation gel filtration. Background RNA binding observed for VLPs HBc1-149, which...
Twelve MAbs were generated by immunization of BALB/c mice with plasma-derived hepatitis B virus surface spherical antigen particles subtype ayw2 (HBsAg/ayw2 genotype D). Their epitopes mapped analysis reactivity HBsAg/ayw2 and HBsAg/adw2 (genotype A) in enzyme immunoassays blots. Mapping was supported nested sets truncated preS2 proteins peptides. Five antibodies S domain-specific, seven preS2-specific 11 had a preference for D. According to our data, group I the three known epitope groups...
Hepatitis C core protein is an attractive target for HCV vaccine aimed to exterminate infected cells. However, although highly immunogenic in natural infection, appears have low immunogenicity experimental settings. We design prototype based on core, and devise immunization regimens that would lead potent anti-core immune responses which circumvent the limitations earlier observed.Plasmids encoding with no translation initiation signal (pCMVcore); Kozak sequence (pCMVcoreKozak); IRES...
The core proteins (HBc) of the hepatitis B virus (HBV) genotypes A, B, C, D, E, F, and G were cloned expressed in Escherichia coli (E. coli), HBc-formed virus-like particles (VLPs) purified with ammonium sulfate precipitation, gel filtration, ion exchange chromatography (IEX). best VLP yield was found for HBc HBV D G. For G, possibility dissociation reassociation maintaining native structure demonstrated. Single-stranded (ss) double-stranded (ds) ribonucleic acid (RNA) successfully packed into VLPs
<i>Objectives:</i> In an attempt to develop virus-like particles (VLPs) as experimental vaccine against human papilloma virus (HPV)-induced tumours, the HPV16 E7 oncoprotein epitopes spanning amino acid (aa) residues 35–98 were expressed on three proteins capable of VLP formation: hepatitis B (HBV) surface (HBs) and core (HBc) antigens, RNA phage fr coats (frCP). <i>Methods:</i> The profile immunoglobulin isotypes induced in Balb/C mice after immunization with...
The major immunodominant region (MIR) and N-terminus of the hepatitis B virus (HBV) core (HBc) protein were used to expose foreign insertions on outer surface HBc virus-like particles (VLPs). additions positively charged arginine-rich C-terminal (CT) domain are usually not exposed VLP surface. Here, we constructed a set recombinant HBcG vectors in which CT arginine stretches substituted by glycine residues. In contrast natural VLPs variants carrying native domain, demonstrated lowered...
A structure-function analysis of the icosahedral RNA bacteriophage fr coat protein (CP) assembly was undertaken using linker-insertion, deletion and substitution mutagenesis. Mutations were specifically introduced into either pre-existing or artificially created restriction enzyme sites within CP gene expressed in Escherichia coli from a recombinant plasmid. This directs synthesis wild type that undergoes self-assembly forms capsid-like particles indistinguishable morphologically...
A set of monoclonal antibodies (mAbs) directed against the preS2 region hepatitis B virus (HBV) surface antigen (HBsAg) was generated by immunization mice with native HBsAg isolated from blood HBV carriers. According to (1) mutual competition binding mAb natural HBsAg, (2) recognition full-length displayed on core particles, (3) synthetic partial peptides, and (4) Western blotting using a fusion protein library truncated fragments different legths, mAbs were assigned two groups which...
Three variants of the major rubella virus (RV) E1 protein virus-neutralizing epitope from position 214 to 285 were exposed on hepatitis B (HBV) C-terminally truncated core (HBcΔ) in a virus-like particle (VLP) vector and produced Escherichia coli. All three chimeras demonstrated VLPs bacterial cell lysates, but only HBcΔ-E1(245-285) correct VLP structure after purification. The other chimeras, HBcΔ-E1(214-285) HBcΔ-E1(214-240), appeared purification as non-VLP aggregates 100 900 nm diameter...
The sequence of the preS domain hepatitis B virus (HBV, genotype D) envelope was inserted into major immunodominant region (MIR) C-terminally truncated HBV core (HBc) protein. In Escherichia coli, HBc-preS fusion protein partially soluble and did not produce particles. Co-expression wild-type HBc as a helper along with led to formation mosaic particles that exhibited HBc, preS1 preS2 antigenicity. Two alternative combinations medium- high-copy plasmids were used for co-expression proteins,...
The available HBV vaccines based on the surface protein are manufactured in yeasts and demonstrate excellent prophylactic but no therapeutic activity thus ineffective against chronic infection. Five different core proteins (HBc)-full length C-terminally truncated-were used for insertion of short, preS1,aa 20-47 long, preS1phil, aa 12-60 + 89-119 fragments. Modified virus-like particles (VLPs) were compared their biotechnological immunological properties. expression level HBc-preS1 was high...
Virus-like particles (VLPs) offer an attractive possibility for the development of vaccines. Recombinant core antigen (HBc) Hepatitis B virus (HBV) was expressed in different systems, and E. coli expression system shown to be effective production HBc VLPs. Here, we used HBV genotype G (HBc/G) as a technologically promising VLP carrier presentation spike RBM nucleocapsid protein-derived peptides SARS-CoV-2 Delta variant subsequent immunological evaluations obtained fusion proteins. The major...