A5038349275- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Botulinum Toxin and Related Neurological Disorders
- Neurological disorders and treatments
- HER2/EGFR in Cancer Research
- Protein purification and stability
- Toxin Mechanisms and Immunotoxins
- Hereditary Neurological Disorders
- Advanced Biosensing Techniques and Applications
- Bacteriophages and microbial interactions
- Nanoparticle-Based Drug Delivery
- Cell Adhesion Molecules Research
- Radiopharmaceutical Chemistry and Applications
- Biochemical and Structural Characterization
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Neuroblastoma Research and Treatments
- Advanced biosensing and bioanalysis techniques
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Respiratory Support and Mechanisms
- Airway Management and Intubation Techniques
- Mast cells and histamine
- CAR-T cell therapy research
- Blood groups and transfusion
University of California, San Francisco
2015-2024
San Francisco General Hospital
2012-2022
Merrimack Pharmaceuticals (United States)
2012-2016
UCSF Helen Diller Family Comprehensive Cancer Center
2005-2015
Association of Perioperative Registered Nurses
2002-2015
University of California System
2001-2014
University of San Francisco
2014
Fox Chase Cancer Center
2006-2012
Georgetown University
2011
Georgetown Lombardi Comprehensive Cancer Center
2011
We describe evidence for a novel mechanism of monoclonal antibody (MAb)-directed nanoparticle (immunoliposome) targeting to solid tumors in vivo. Long-circulating immunoliposomes targeted HER2 (ErbB2, Neu) were prepared by the conjugation anti-HER2 MAb fragments (Fab' or single chain Fv) liposome-grafted polyethylene glycol chains. fragment did not affect biodistribution long-circulating properties i.v.-administered liposomes. However, antibody-directed also increase tumor localization...
A large library of phage-displayed human single-chain Fv antibodies (scFv), containing 6.7 × 10 9 members, was generated by improving the steps construction. Fourteen different protein antigens were used to affinity select from this library. panel specific isolated with each antigen, and contained an average 8.7 scFv. Measurements antibody–antigen interactions revealed several affinities below 1 nM, comparable observed during secondary murine immune response. In particular, four scFv...
ABSTRACT Clostridium botulinum is a taxonomic designation for many diverse anaerobic spore-forming rod-shaped bacteria that have the common property of producing neurotoxins (BoNTs). The BoNTs are exoneurotoxins can cause severe paralysis and death in humans other animal species. A collection 174 C. strains was examined by amplified fragment length polymorphism (AFLP) analysis sequencing 16S rRNA gene BoNT genes to examine genetic diversity within this This contained representatives each...
Antibody drugs are widely used in cancer therapy, but conditions to maximize tumor penetration and efficacy have yet be fully elucidated. In this study, we investigated the impact of antibody binding affinity on targeting with variants that recognize same epitope. Specifically, compared four derivatives C6.5 monoclonal (mAb), which recognizes HER2 epitope (monovalent K(D) values ranging from 270 0.56 nmol/L). Moderate was associated highest accumulation at 24 120 hours after intravenous...
The North American Contact Dermatitis Group (NACDG) tests patients who have suspected allergic contact dermatitis with a broad series of screening allergens, and publishes periodic reports its data.To report the NACDG patch-test results from January 1, 2005, to December 31, 2006, compare pooled test data previous 10 years.Standardized patch testing 65 allergens was used at 13 centers in America. Chi-square statistics were utilized for comparisons data.NACDG patch-tested 4,454 patients; 12.3%...
The botulinum neurotoxins (BoNTs) cause the paralytic human disease botulism and are one of highest-risk threat agents for bioterrorism. To generate a pharmaceutical to prevent or treat botulism, monoclonal antibodies (mAbs) were generated by phage display evaluated neutralization BoNT serotype A (BoNT/A) in vivo . Although no single mAb significantly neutralized toxin, combination three mAbs (oligoclonal Ab) 450,000 50% lethal doses BoNT/A, potency 90 times greater than hyperimmune...
We hypothesized that immunoliposomes (ILs) target epidermal growth factor receptor (EGFR) and/or its truncated variant EGFRvIII can be constructed to provide efficient intracellular drug delivery in tumor cells overexpressing these receptors. Monoclonal antibody fragments included Fab' derived from C225, which binds both EGFR and EGFRvIII, or novel anti-EGFR scFv C10, only. were covalently linked liposomes containing various reporters drugs. ILs evaluated for specific binding,...
Objectives To estimate the frequency of acute withdrawal syndrome related to administration analgesic and sedative medications in mechanically ventilated adult intensive care unit (ICU) patients; identify associated clinical factors. Design Retrospective review medical records. Setting An trauma/surgical ICU an urban Level I trauma center. Patients Twenty-eight patients requiring >7 days care. Interventions None. Measurements Main Results Daily doses all opioid, sedative, hypnotic, major...
The botulinum neurotoxins (BoNTs) are category A biothreat agents which have been the focus of intensive efforts to develop vaccines and antibody-based prophylaxis treatment. Such approaches must take into account extensive BoNT sequence variability; seven serotypes differ by up 70% at amino acid level. Here, we analyzed 49 complete published sequences BoNTs show that all toxins also exhibit variability within ranging between 2.6 31.6%. To determine impact such differences on immune...
In this prospective study of 45 patients, we tested the hypothesis that markedly elevated levels plasma von Willebrand antigen (vWf-Ag) a marker endothelial cell injury, might predict development acute lung injury in patients with nonpulmonary sepsis syndrome. Acute was quantified on four-point scoring system. At time entry into study, none had evidence injury. Subsequently, 15 developed and 30 did not develop The mean vWf-Ag level who compared (588 +/- 204 vs. 338 196, percentage control, P...
To compare the effects of continuous positive airway pressure (CPAP) with those ambient end-expiratory (T-tube) on lung mechanics and blood gas exchange, transpulmonary (Ptp), tidal volume (VT), respiratory frequency, arterial oxygen carbon dioxide tensions were measured in 16 spontaneously breathing patients recovering from acute failure. These variables during through a T-tube; 18, 12, 6 cmH2O CPAP; again T-tube. During all levels CPAP, mean effective compliance (Ceff) was higher total...
Abstract In recent work, the polymerase chain reaction (PCR) has been used to amplify rearranged mouse and human immunoglobulin heavy χ light variable (V) genes. Here we have optimized design of PCR primers for V genes them cDNA from peripheral blood lymphocytes. Cloning sequencing revealed a diverse repertoire genes, presence members each gene family. After alignment sequences, identified region conserved within families, but differing between this family‐specific oligonucleotide probes.
The authors studied 12 surgical patients in the intensive care unit post coronary artery bypass graft surgery and ten nonsurgical with chronic heart failure to determine usefulness of pulmonary arterial wedge pressure as an indicator left ventricular preload. Left end diastolic volume was derived from concomitant determination ejection fraction (gated blood pool scintigraphy) stroke (determined thermodilution cardiac output). In patients, there a significant correlation between changes...