A5044774484- HIV Research and Treatment
- HIV-related health complications and treatments
- HIV/AIDS Research and Interventions
- Immune cells in cancer
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Immune Cell Function and Interaction
- Diabetes and associated disorders
- Cancer, Hypoxia, and Metabolism
- Long-Term Effects of COVID-19
- Tryptophan and brain disorders
- T-cell and B-cell Immunology
- Liver Disease Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Bioinformatics and Genomic Networks
- Epigenetics and DNA Methylation
- Metabolomics and Mass Spectrometry Studies
- HIV/AIDS drug development and treatment
- Phagocytosis and Immune Regulation
- Diet and metabolism studies
- Silk-based biomaterials and applications
- Endoplasmic Reticulum Stress and Disease
- Wound Healing and Treatments
Karolinska Institutet
2017-2025
Harvard University
2024
Boston Children's Hospital
2024
Riga Stradiņš University
2022
Russian Academy of Sciences
2022
Czech Academy of Sciences, Institute of Microbiology
2021
Institute of Cell Biology
2017
How severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infections engage cellular host pathways and innate immunity in infected cells remains largely elusive. We performed an integrative proteo-transcriptomics analysis SARS-CoV-2 Huh7 to map the response invading virus over time. identified four pathways, ErbB, HIF-1, mTOR TNF signaling, among others that were markedly modulated during course of infection vitro. Western blot validation downstream effector molecules these revealed a...
The ART program in low- and middle-income countries (LMIC) like India, follows a public health approach with standardized regimen for all people living HIV (PLHIV). Based on the evidence from high-income (HIC), risk of an enhanced, accentuated onset premature-aging or age-related diseases has been observed PLHIV. However, very limited data is available residual inflammation immune activation populations who are first-generation anti-HIV drugs zidovudine lamivudine that have more toxic side...
The clinical outcome and disease severity in coronavirus 2019 (COVID-19) are heterogeneous, the progression or fatality of cannot be explained by a single factor like age comorbidities. In this study, we used system-wide network-based system biology analysis using whole blood RNA sequencing, immunophenotyping flow cytometry, plasma metabolomics, single-cell-type metabolomics monocytes to identify potential determinants COVID-19 at personalized group levels. Digital cell quantification...
Abstract Despite successful combination antiretroviral therapy (cART), persistent low-grade immune activation together with inflammation and toxic drugs can lead to long-lasting metabolic flexibility adaptation in people living HIV (PLWH). Our study investigated alterations the plasma profiles by comparing PLWH on long-term cART(>5 years) matched HIV-negative controls (HC) two cohorts from low- middle-income countries (LMIC), Cameroon, India, respectively, understand system-level...
The pathogenesis and host-viral interactions of the Crimean–Congo hemorrhagic fever orthonairovirus (CCHFV) are convoluted not well evaluated. Application multi-omics system biology approaches, including biological network analysis in elucidating complex response, interrogates viral pathogenesis. present study aimed to fingerprint system-level alterations during acute CCHFV-infection cellular immune responses productive CCHFV-replication vitro. We used system-wide network-based peripheral...
Cells of the myeloid lineage, particularly monocytes and macrophages, play a key role in HIV infection by contributing to viral replication, immune response, maintaining balance during suppressive therapy. We hypothesized that metabolic reprogramming altered chemokine signaling people living with (PWH) on long-term antiretroviral therapy (ART) affect monocyte transport polarization due ongoing inflammation. Therefore, present study aimed identify mechanism impaired monocyte/macrophage...
Abstract Risk stratification using multi‐omics data deepens understanding of immunometabolism in successfully treated people with HIV (PWH) is inadequately explained. A personalized medicine approach integrating blood cell transcriptomics, plasma proteomics, and metabolomics employed to identify the mechanisms immunometabolic complications prolonged PWH from COCOMO cohort. Among PWHs, 44% are at risk experiencing identified network‐based patient method. Utilizing advanced machine learning...
The immune response to SARS-CoV-2 is highly heterogeneous, with interferon (IFN)-stimulated gene (ISG) expression playing a dual role in antiviral defense and dysregulation. To understand the broader implications of IFN-driven responses, we analyzed whole-blood transcriptomics, plasma proteomics, metabolomics, cell profiling COVID-19 patients uninfected controls. Patients were stratified into low (LIS), moderate (MIS), high (HIS) ISG clusters, independent acute disease severity. HIS...
Human immunodeficiency virus type 1 (HIV-1) infection is a chronic condition, where viral DNA integrates into the genome. Latently infected cells form persistent, heterogeneous reservoir that at any time can reactivate integrated HIV-1. Here we confirmed latently from HIV-1 positive study participants exhibited active transcription but without production of mature spliced mRNAs. To elucidate mechanisms behind this employed primary latency models to establishment and maintenance. We...
HIV-1 elite controllers (EC) are a rare but heterogeneous group of HIV-1-infected individuals who can suppress viral replication in the absence antiretroviral therapy. The mechanisms how EC achieve undetectable loads remain unclear. This study aimed to investigate host plasma metabolomics and targeted proteomics Swedish cohort including treatment-naïve viremic progressors (VP) as well HIV-negative (HC) get insights into phenotype. Metabolites belonging antioxidant defense had higher levels...
Why people with HIV-1 on ART (PWH ) display convoluted metabolism and immune cell functions during prolonged suppressive therapy is not well evaluated. In this study, we aimed to address question using multiomics methodologies investigate immunological metabolic differences between PWH negative individuals (HC).Cross-sectional study.Untargeted targeted metabolomics was performed gas liquid chromatography/mass spectrometry, proteomics Olink inflammation panel plasma samples. The cellular...
Identifying the source and dynamics of persistent HIV-1 at single-cell resolution during cART is crucial for design strategies to eliminate latent reservoir. An assay measure that can distinguish inducible from defective proviruses with high precision essential evaluate efficacy cure efforts but presently lacking. The primary aim this study was therefore identify transcription translation competent latently infected cells through detection biomolecules are dependent on transcriptional...
Genome-scale metabolic models (GSMMs) can provide novel insights into reprogramming during disease progression and therapeutic interventions. We developed a context-specific system-level GSMM of people living with HIV (PLWH) using global RNA sequencing data from PBMCs suppressive viremia either by natural (elite controllers, PLWH EC ) or drug-induced (PLWH ART control. This was compared HIV-negative controls (HC) to comprehensive systems-level metabo-transcriptomic characterization....
Crimean-Congo hemorrhagic fever (CCHF) caused by CCHF virus (CCHFV) is one of the epidemic-prone diseases prioritized World Health Organisation as public health emergency with an urgent need for accelerated research. The trajectory host response against CCHFV multifarious and remains unknown. Here, we reported temporal spectrum pathogenesis following infection using genome-wide blood transcriptomics analysis followed advanced systems biology analysis, immune-pathogenic alterations,...
Abstract Viruses hijack host metabolic pathways for their replicative advantage. Several observational trans-omics analyses associated carbon and amino acid metabolism in coronavirus disease 2019 (COVID-19) severity patients but lacked mechanistic insights. In this study, using patient- derived multi-omics data vitro infection assays, we aimed to understand i) role of key severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) reproduction ii) its association with severity. Our suggests...
HIV-1 infection induces a chronic inflammatory environment not restored by suppressive antiretroviral therapy (ART). As of today, the effect viral suppression and immune reconstitution in people living with (PLWH) has been well described but completely understood. Herein, we show how PLWH who naturally control virus (PLWHEC) have reduced proportion CD4+CCR6+ CD8+CCR6+ cells compared to on ART (PLWHART) negative controls (HC). Expression CCR2 was both CD4+, CD8+ classical monocytes PLWHEC...
Abstract How Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infections engage cellular host pathways and innate immunity in infected cells remain largely elusive. We performed an integrative proteo-transcriptomics analysis SARS-CoV-2 HuH7 to map the response invading virus over time. identified four pathways, ErbB, HIF-1, mTOR TNF signaling, among others that were markedly modulated during course of infection vitro . Western blot validation downstream effector molecules these...
The potential of multifunctional wound heal biomaterial relies on the optimal content therapeutic constituents as well desirable physical, chemical and biological properties to accelerate healing process. Formulating biomaterials such Amnion or collagen based scaffolds with natural products offer an affordable strategy develop dressing material high efficiency in wounds. Using image phenotyping quantification, we screened product derived bioactive compounds for modulators type I III...