A5081230925- Gut microbiota and health
- HIV-related health complications and treatments
- Extracellular vesicles in disease
- Clostridium difficile and Clostridium perfringens research
- HIV Research and Treatment
- Diet and metabolism studies
- MicroRNA in disease regulation
- Metabolomics and Mass Spectrometry Studies
- Tryptophan and brain disorders
- Dysphagia Assessment and Management
- Helicobacter pylori-related gastroenterology studies
- Inflammatory Biomarkers in Disease Prognosis
- Circular RNAs in diseases
- Drug Transport and Resistance Mechanisms
- Intensive Care Unit Cognitive Disorders
- COVID-19 Clinical Research Studies
- Immune cells in cancer
- Diabetes and associated disorders
- Amoebic Infections and Treatments
- Nutritional Studies and Diet
- Blood disorders and treatments
- Bacterial Infections and Vaccines
- Immunodeficiency and Autoimmune Disorders
- Microbial Metabolic Engineering and Bioproduction
- Bioinformatics and Genomic Networks
Oslo University Hospital
2017-2025
University of Oslo
2018-2022
Western Norway Regional Health Authority
2017
Current methods for characterisation of extracellular vesicles (EVs) need further standardisation in order to obtain an acceptable level data comparability. Size and concentration EVs can be determined by nanoparticle tracking analysis (NTA). However, both the heterogeneity choice instrument settings may cause appreciable analytical variation. Intra-assay (within-day, n = 6) inter-assay (day-to-day, variations (coefficient variation, % CV) different preparations artificial or beads were...
There is a long-lasting need for non-invasive, more accurate diagnostic techniques when evaluating primary Sjögren's syndrome (pSS) patients. Incorporation of additional diagnostics involving screening disease-specific biomarkers in biological fluid promising concept that requires further investigation. In the current study we aimed to explore novel disease saliva and tears from pSS patients.Liquid chromatography-mass spectrometry (LC-MS) was performed on stimulated whole 27 patients 32...
Extracellular vesicles (EVs) are a heterogeneous population of biological particles released by cells. They represent an attractive source potential biomarkers for early detection diseases such as cancer. However, it is critical that sufficient amounts EVs can be isolated and purified in robust reproducible manner. Several isolation methods seem to produce distinct populations exist, making data comparability difficult. While some induce cellular stress may affect both the quantity function...
Abstract Aims Recent reports have suggested that patients with heart failure (HF) an altered gut microbiota composition; however, associations diet remain largely uninvestigated. We aimed to explore differences in the between HF reduced ejection fraction and healthy controls, focusing on disease severity. Methods results The composition of two cross‐sectional cohorts (discovery, n = 40 validation, 44) systolic controls ( 266) was characterized by sequencing bacterial 16S rRNA gene. overall...
Although coronavirus disease 2019 (COVID-19) is primarily a respiratory infection, mounting evidence suggests that the gastrointestinal tract involved in disease, with gut barrier dysfunction and microbiota alterations being related to severity. Whether these persist are long-term remains unknown.Plasma was collected during hospital admission after 3 months from NOR-Solidarity trial (n = 181) analyzed for markers of inflammation. At 3-month follow-up, pulmonary function assessed by measuring...
Gut microbiota alterations have been reported in hospitalized COVID-19 patients, with reduced alpha diversity and altered composition related to respiratory failure. However, data regarding gut mortality are scarce.Rectal swabs for analyses were collected within 48 h after hospital admission (baseline; n = 123) three-month post-admission (n 50) a subset of patients included the Norwegian SARS-CoV2 cohort study. Samples analysed by sequencing 16S rRNA gene. at baseline assessed relation need...
Abstract Background Interactions between the gut microbiota, diet, and host metabolism contribute to development of cardiovascular disease, but a firm link disease-specific microbiota alterations circulating metabolites is lacking. Methods We performed shot-gun sequencing on 235 samples from 166 HF patients 69 healthy control samples. Separate plasma controls ( n = 53) were used for comparison imidazole propionate (ImP) levels. Taxonomy functional pathways shotgun data was assigned using...
Abstract Background The impact of gut microbiota and its metabolites on coronary artery disease (CAD) in people with human immunodeficiency virus (PWH) is unknown. Emerging evidence suggests that imidazole propionate (ImP), a microbial metabolite, linked cardiometabolic diseases. Methods Fecal samples from participants the Copenhagen Comorbidity HIV infection (COCOMO) study were processed for 16S rRNA sequencing ImP measured liquid chromatography-tandem mass spectrometry. CAD severity was...
We aimed to identify a human immunodeficiency virus (HIV)-related microbiota signature, independent of sexual preferences and demographic confounders, in order assess possible impact the microbiome on metabolic comorbid conditions.Bacterial 16S ribosomal RNA analyses were performed stool samples from 405 HIV-infected 111 uninfected participants Copenhagen Comorbidity HIV Infection (COCOMO) study. Individuals stratified according behavior (men who have sex with men [MSM] vs non-MSM).After...
Abstract Risk stratification using multi‐omics data deepens understanding of immunometabolism in successfully treated people with HIV (PWH) is inadequately explained. A personalized medicine approach integrating blood cell transcriptomics, plasma proteomics, and metabolomics employed to identify the mechanisms immunometabolic complications prolonged PWH from COCOMO cohort. Among PWHs, 44% are at risk experiencing identified network‐based patient method. Utilizing advanced machine learning...
Abstract The gut microbiome contributes to the variation of blood lipid levels, and secondary bile acids are associated with effect statins. Yet, our knowledge how statins, one most common drug groups, affect human is scarce. We aimed characterize rosuvastatin on composition inferred genetic content in stool samples from a randomized controlled trial ( n = 66). No taxa were significantly altered by during study. However, rosuvastatin-treated participants showed reduction collective potential...
Multiomics technologies improve the biological understanding of health status in people living with HIV on antiretroviral therapy (PWH). Still, a systematic and in-depth characterization metabolic risk profile during successful long-term treatment is lacking. Here, we used multi-omics (plasma lipidomic, metabolomic, fecal 16 S microbiome) data-driven stratification to identify at-risk within PWH. Through network analysis similarity fusion (SNF), identified three groups PWH (SNF-1–3): healthy...
Alterations in the gut microbiome have been associated with inflammation and increased cardiovascular risk HIV-infected individuals. The aim of this study was to investigate effects probiotic strain Lactobacillus rhamnosus GG (LGG) on intestinal inflammation, microbiota composition, systemic markers microbial translocation individuals.This prospective, clinical interventional trial included 45 individuals [15 combination antiretroviral treatment (cART) naive 30 cART treated] who ingested LGG...
Triglycerides (TG) and their major transport lipoprotein in the circulation (VLDL) appear to be related inflammation. Patients with common variable immunodeficiency (CVID) have inflammatory complications associated gut microbial dysbiosis. We hypothesized that CVID patients disturbed TG/VLDL profiles these clinical characteristics.We measured plasma concentrations of TGs, markers, lipopolysaccharide (LPS) 95 28 healthy controls. Additionally, 40 patients, we explored profiling, fatty acid,...
Abstract The short-chain fatty acid (SCFA) butyric maintains a healthy gut barrier and vascular endothelium. We aimed to investigate the association between fecal acid, carotid atherosclerosis risk factors for ischemic stroke. Patients with severe (i.e. ≥ 50% stenosis) (n = 43) were compared controls 38). analyzed SCFAs by gas chromatography, microbiota composition 16S rRNA sequencing, markers of damage inflammasome activation immunoassay, plasma ultra-high performance liquid...
The aim of the study was to investigate association between human immunodeficiency virus (HIV)-related gut microbiota changes, alterations in kynurenine (Kyn) pathway tryptophan (Trp) metabolism, and visceral adipose tissue context HIV infection.Three hundred eighty-three people with (PWH) were included from Copenhagen comorbidity infection (COCOMO) study. Gut composition analyzed by 16S ribosomal ribonucleic acid sequencing. Plasma metabolites liquid chromatography-tandem mass spectrometry....
Individuals with familial hypercholesterolemia (FH) undergo an aggressive treatment cholesterol-lowering drugs to prevent coronary heart disease. Recent evidence suggests interplay between the gut microbiota, blood lipid levels and lipid-lowering drugs, but this has yet be studied in individuals FH. The objective of study was characterize microbiota examine if effects omega-3 polyunsaturated fatty acids (PUFAs) on lipids act through modification microbiome. composition FH (N = 21) healthy...
Abstract HIV and type 2 diabetes (T2D) are both associated with gut microbiota alterations, low-grade endotoxemia increased cardiovascular risk. We investigated the potential role of plasma extracellular vesicles (EVs) in relation to these processes. Plasma EVs were isolated by size exclusion chromatography fasting individuals T2D (n = 16), only 14), 20) or healthy controls 19), characterized transmission electron microscopy, western blot, nanoparticle tracking analysis quantitative...
Atherosclerosis and its consequences cause considerable morbidity mortality world-wide. We have previously shown that expression of the DNA glycosylase NEIL3 is regulated in human atherosclerotic plaques, NEIL3-deficiency enhances atherogenesis Apoe-/- mice. Herein, we identified a time point prior to quantifiable differences atherosclerosis between Apoe-/-Neil3-/- mice Mice at this age were selected explore metabolic pathophysiological processes preceding extensive NEIL3-deficient...
Extracellular vesicles (EVs) released by tumor cells can directly or indirectly modulate the phenotype and function of immune microenvironment locally at distant sites. The uptake circulating EVs responses human monocytes in vitro may provide new insights into underlying biology invasive metastatic processes cancer. Although a mixed population is obtained with most isolation techniques, we predominantly isolated exosomes (small EVs) microvesicles (medium from SW480 colorectal cancer cell...
Abstract Objective Although COVID-19 is primarily a respiratory infection, mounting evidence suggests that the GI tract involved in disease, with gut barrier dysfunction and microbiota alterations being related to disease severity. Whether these persist could be long-term unknown. Design From NOR-Solidarity trial (n=181), plasma was collected during hospital admission after three months, analyzed for markers of inflammation. At three-month follow-up, pulmonary function assessed by measuring...
Abstract Multiomics technologies improve the biological understanding of health status in people living with HIV on antiretroviral therapy (PLWH ART ). Still, a systematic and in-depth characterization metabolic risk profile during successful long-term treatment is lacking. Here, we used multi-omics (plasma lipidomic metabolomic, fecal 16s microbiome) data-driven stratification to identify at-risk within PLWH . Through network analysis similarity fusion (SNF), identified three groups (SNF-1...
Abstract Background: HIV and type 2 diabetes (T2D) are both associated with gut microbiota alterations, low-grade endotoxemia increased cardiovascular risk. We investigated the potential role of plasma extracellular vesicles (EVs) in relation to these processes. Materials methods: Plasma EVs were isolated by size exclusion chromatography fasting individuals T2D (n=16), only (n=14), (n=20) or healthy controls (n=19), characterized transmission electron microscopy, western blot, nanoparticle...