Extracellular vesicles (EVs) are a heterogeneous population of biological particles released by cells. They represent an attractive source potential biomarkers for early detection diseases such as cancer. However, it is critical that sufficient amounts EVs can be isolated and purified in robust reproducible manner. Several isolation methods seem to produce distinct populations exist, making data comparability difficult. While some induce cellular stress may affect both the quantity function...

Oral cancer is one of the most common forms head and neck cancers. squamous cell carcinoma (OSCC) accounts for more than 90% oral malignancies. The molecular pathogenesis OSCC complex as it involves altered expression specific genes proteins, but also comprises changes in metabolic processes. It suggested that extracellular vesicles (EVs) released by cells may contribute to development metastasis recruiting changing phenotype normal surround tumor. aim project was characterize effect EVs on...

ABSTRACT Background Updated and comprehensive epidemiological data on odontogenic tumors are not available for the Nordic countries. The aim of study was to retrospectively examine prevalence clinical radiological characteristics primary over a 10‐year period in Sweden, Norway, Finland, Denmark. Methods Primary diagnosed by oral pathologists from 2010 2020 at nine different diagnostic laboratories Denmark were identified. Using histopathology referrals their corresponding histopathological...

This study aimed to investigate the impact of hydrogen peroxide-induced oxidative stress on protein expression profiles submandibular and parotid acinar cells using a proteomic approach. We sought evaluate how might contribute salivary gland dysfunction whether two glands respond differently. Immortalized rat (PG) (SMG) epithelial cell lines were exposed 50 µM 150 peroxide for 24 hr, followed by identification quantification via liquid chromatography-mass spectrometry. Immunofluorescence...

Background The majority of oral cavity cancers arise in the tongue. aim this study was to evaluate prognostic value tumor budding tongue squamous cell carcinoma, both as a separate variable and combination with depth invasion. We also assessed impact 8th edition American Joint Committee on Cancer's TNM classification (TNM8), where invasion (DOI) supplements diameter size (T) categorization. Methods Patients diagnosed primary carcinoma were evaluated retrospectively. Spearman bivariate...

Extracellular vesicles (EVs), are important for intercellular communication in both physiological and pathological processes. To explore the potential of cancer derived EVs as disease biomarkers diagnosis, monitoring, treatment decision, it is necessary to thoroughly characterize their biomolecular content. The aim study was compare protein content from three different cell lines search a specific molecular signature, with emphasis on proteins related carcinogenic process. Oral squamous...

Aims: To evaluate the expression of p75 neurotrophin receptor (p75 NTR ) in oral squamous cell carcinoma (OSCC). The results were related to tumour node metastasis (TNM) stage, World Health Organization (WHO) grade, invasive front grading (IFG) and prognosis. Methods results: Immunohistochemically, was assessed 53 T1‐T2 OSCCs. Clinical data recorded prospectively. end‐point disease‐free survival. All tumours expressed , this expression, both central/superficial areas at front, associated...

Background The purpose of this study was to investigate acute normal tissue responses in the head and neck region following proton- or X-irradiation a murine model.Materials methods Female C57BL/6J mice were irradiated with protons (25 60 MeV) X-rays (100 kV). radiation field covered oral cavity major salivary glands. For protons, two different treatment plans used, either Bragg Peak middle mouse (BP) outside (transmission mode; TM). Delivered physical doses 41, 45, 65 Gy given 6, 7, 10...

Introduction Normal oral fibroblasts (NOFs) are located in the connective tissue of mucosa. The NOFs play an important role wound healing, tumor progression, and metastasis. They subjected to influence by external internal stimuli, among them extracellular vesicles (EVs), that considered as players cell communication, especially carcinogenesis metastatic processes. During tumorigenesis, stromal may undergo activation into cancer-associated (CAFs) modify their phenotype provide pro-oncogenic...

Brusevold IJ, Søland TM, Khuu C, Christoffersen T, Bryne M. Nuclear and cytoplasmic expression of Met in oral squamous cell carcinoma an organotypic cancer model. Eur J Oral Sci 2010; 118: 342–349. © 2010 The Authors. Journal compilation Met, the hepatocyte growth factor receptor, is important transducing signals for tumour metastasis. aim this study was to examine pattern its value as a prognostic carcinomas (OSCCs). material consisted 53 OSCCs five healthy controls from normal mucosa...

Abstract Introduction Moldova, Belarus, and Armenia are post-Soviet countries with a high rate of heavy smokers relatively age-standardized incidence oral cancer. However, to our knowledge, there is lack available information on dentists’ knowledge prevention cancer in the question. Accordingly, this study aimed assess opinions, practices related mucosal examination among dentists Armenia. Methods This was multi-country, cross-sectional based self-administered questionnaire. A structured...

Cytokines are mediators of inflammation that could lead to fibrosis. The aim was monitor cytokine levels in saliva and serum after locally fractionated radiotherapy the head neck mice investigate associations with salivary gland fibrosis hyposalivation. C57BL/6 were randomized sham or X-ray irradiation 66 Gy 10 fractions over 5 days. Blood collected on days -7, 5, 35, 80, 105 following analysis. harvested submandibular assessed for presence Decision tree regression analysis used whether...

Proton therapy gives less dose to healthy tissue compared conventional X-ray therapy, but systematic comparisons of normal responses are lacking. The aim this study was investigate late in the salivary glands following proton- or X-irradiation head and neck mice. Moreover, we aimed at investigating molecular by monitoring cytokine levels serum saliva. Female C57BL/6J mice underwent local fractionated irradiation with protons X-rays maximally tolerated acute level. Saliva were collected...

Background: Histomorphological grading at the invasive front of oral squamous cell carcinomas (OSCCs) may provide useful prognostic information. In present study, we investigated presence and value activated phosphorylated extracellular signal‐regulated kinases 1 2 (p‐ERK1/2) cyclo‐oxygenase‐2 (COX‐2) both in central/superficial parts OSCCs. Methods: Using immunohistochemistry, assessed p‐ERK1/2 COX‐2 53 early stage Clinical data were recorded prospectively. The end point was disease‐free...

Abstract Objectives The presence of and the causative role high‐risk human papilloma virus (HPV) is a subject controversy in oral squamous cell carcinoma (OSCC). disagreement can be related to misclassification OSCC as oropharyngeal and/or lack standard detection methods. This study aimed examine transcriptionally active HPV homogenous Norwegian cohort primary second mobile tongue (oral carcinoma—OTSCC). Methods Tissue microarrays containing formalin‐fixed paraffin‐embedded cores 146 OTSCC...

Radiotherapy (RT) of head and neck (H&N) cancer is known to cause both early- late-occurring toxicities. To better appraise normal tissue responses their dependence on treatment parameters such as radiation field type, well dose fractionation scheme, a preclinical model with relevant endpoints required. 12-week old female C57BL/6 J mice were irradiated 100 or 180 kV X-rays total doses ranging from 30 85 Gy, given in 10 fractions over 5 days. The covered the oral cavity, swallowing structures...

This study aimed to evaluate the prognostic significance of expression levels involucrin (IVL), cytokeratin (CK)-10 and -13 at different intratumor sites (tumor center invading area) oral tongue squamous cell carcinoma (OTSCC).IVL, CK13 CK10 were examined in a multicenter cohort 146 OTSCCs using immunohistochemistry. External mRNA datasets used for analysis and/or validate survival associations.External transcriptomic showed downregulation IVL KRT13 malignancies including OTSCC as compared...

Brusevold IJ, Husvik C, Schreurs O, Schenck K, Bryne M, Søland TM. Induction of invasion in an organotypic oral cancer model by CoCl 2 , a hypoxia mimetic. Eur J Oral Sci 2010; 118: 168–176. © 2010 The Authors. Journal compilation Invasion is hallmark malignancy. aim this study was to develop vitro that can be used for experimental studies cell invasion. constructed growing squamous carcinoma (OSCC) cells on collagen matrix which normal human fibroblasts were incorporated....

Numerous studies have been presented on histological grading of oral squamous cell carcinomas (OSCC) for predicting survival, but uncertainty their usefulness rises due to discordances results. A scoring system should be robust and well validated, intra- interrater agreement can used as a tool visualize the strength reproducibility.Here, we present an inter-observer study evaluation OSCC using some most common histopathological parameters. The observers were from different Norwegian...

Background Secretion of saliva is controlled by autonomic nerve signals via regulation Ca 2+ -dependent ion transport across acinar cell membranes. Oxidative stress may affect this process, leading to a decrease in production. This study investigates elements the regulatory pathway and their vulnerability hydrogen peroxide-induced oxidative stress. Methods Rat parotid submandibular salivary gland lines were exposed different peroxide concentrations simulate Cell viability intracellular...

Abstract In S candinavia, as in many E uropean countries, most patients consult their general dentist once a year or more. This gives the unique opportunity and an obligation to make early diagnosis of oral diseases, which is beneficial for both patient society. Thus, must have knowledge clinical symptoms, local systemic signs differential diagnoses accurate diagnosis. The be competent selecting appropriate diagnostic tests, example, tissue biopsy microbiological samples, conducting them...