Intrauterine growth restriction (IUGR) is a serious pathological complication associated with compromised fetal development during pregnancy. The aim of the study was to broaden knowledge about transcriptomic complexity human placenta by identifying genes potentially involved in IUGR pathophysiology. RNA-Seq data were used profile protein-coding genes, detect alternative splicing events (AS), single nucleotide variant (SNV) calling, and RNA editing sites prediction IUGR-affected placental...

Oral cancer is one of the most common forms head and neck cancers. squamous cell carcinoma (OSCC) accounts for more than 90% oral malignancies. The molecular pathogenesis OSCC complex as it involves altered expression specific genes proteins, but also comprises changes in metabolic processes. It suggested that extracellular vesicles (EVs) released by cells may contribute to development metastasis recruiting changing phenotype normal surround tumor. aim project was characterize effect EVs on...

BACKGROUND Leiomyomas are common benign uterine tumors (BUMTs) with diverse histopathological subtypes and variable clinical presentations. While most asymptomatic, some cause significant morbidity, including abnormal bleeding, infertility, pain. Hydropic leiomyomas (HLMs) rare variants histopathologically characterized by zonal edema may pose diagnostic challenges, particularly when located in atypical sites such as the retroperitoneal space. This report presents a case of HLM strong...

The human placenta is a particular organ that inseparably binds the mother and fetus. proper development survival of conceptus relies on essential interplay between maternal fetal factors involved in cooperation within placenta. In our study, high-throughput sequencing (RNA-seq) was applied to analyze global transcriptome during uncomplicated pregnancies. RNA-seq utilized identify pattern gene expression placentas (N = 4) from women single twin During analyses, we obtained 228,044...

Development of particular structures and proper functioning the placenta are under influence sophisticated pathways, controlled by expression substantial genes that additionally regulated long non-coding RNAs (lncRNAs). To date, profile lncRNA in human term has not been fully established. This study was conducted to characterize verify whether there differences transcriptomic between sex fetus pregnancy multiplicity. RNA-Seq data were used profile, quantify, classify lncRNAs placenta. The...

While glucose is the primary fuel for fetal growth, placenta utilizes majority of taken up from maternal circulation. Of facilitative transporters in placenta, SLC2A8 (GLUT8) thought to primarily function as an intracellular transporter; however, its trophoblast cells has not been determined. To gain insight into lentiviral-mediated RNA interference (RNAi) was performed human first-trimester cell line ACH-3P. Non-targeting sequence controls (NTS RNAi; n = 4) and RNAi (n infected ACH-3P were...

Introduction Normal oral fibroblasts (NOFs) are located in the connective tissue of mucosa. The NOFs play an important role wound healing, tumor progression, and metastasis. They subjected to influence by external internal stimuli, among them extracellular vesicles (EVs), that considered as players cell communication, especially carcinogenesis metastatic processes. During tumorigenesis, stromal may undergo activation into cancer-associated (CAFs) modify their phenotype provide pro-oncogenic...

Anti-Müllerian hormone (AMH) is a commonly known factor secreted by Sertoli cells, responsible for regression of the Müllerian ducts in male fetuses. AMH has also other functions humans. In vivo and vitro studies have shown that inhibits cell cycle induces apoptosis cancers with receptors. The aim study was to assess whether tissue pre-cancerous states endometrium (PCS) various histopathologic types endometrial cancer (EC) exhibit presence AMH. We aimed investigate potential protein concerns...

Deep vein thrombosis (DVT) is a severe disease affecting the human venous system, accompanied by high morbidity and mortality rates caused early late complications. The study aimed at analyzing changes in transcriptome of femoral DVT porcine model based on formation thrombus vivo. was performed 11 castrated male pigs: A formed each left six animals; remaining five served as control group. Total RNA isolated from veins experimental animals. High-throughput sequencing used to analyze global...

Bisphenol A (BPA) is an environmental toxin widely used in the production of polycarbonate plastics. correlation exists between BPA tissue contamination and occurrence pathological conditions, including cancer. First-passage detoxification high amounts liver promotes hepatotoxicity morphological alterations this organ, but there a lack knowledge about molecular mechanisms underlying these phenomena. This prompted us to investigate changes transcriptomics 3-month-old female mice exposed (50...

Kisspeptin (KISS) is a natural peptide—discovered in 1996 as factor inhibiting the ability to metastasize malignant melanoma. This protein plays also regulatory role process of puberty, menstrual cycle, spermatogenesis, implantation and development human placenta. The present study aimed evaluate expression KISS its receptor GPR54 endometrial cancer (EC) tissue, depending on histological type cancer, stage, various demographic characteristics, clinical conditions 214 hysterectomy patients....

Anti-Müllerian hormone (AMH) is responsible for the Müllerian ducts’ regression in male fetuses. In cells of cancers with AMH receptors (AMHRII), induces cell cycle arrest or apoptosis. As occurs naturally and does not exhibit significant side effects while reducing neoplastic colonies, it can be considered as a potential therapeutic agent cancer treatment. The purpose this study was to assess AMHRII expression endometrial (EC) correlation various demographic data clinical conditions....

Impaired fetal growth is one of the most important causes prematurity, stillbirth and infant mortality. The pathogenesis idiopathic restriction (FGR) poorly understood but thought to be multifactorial comprise a range genetic causes. This research aimed investigate non-coding RNAs (lncRNAs) in placentas male female fetuses affected by FGR. RNA-Seq data were analyzed detect lncRNAs, their potential target genes circular (circRNAs); differential analysis was also performed. multilevel...

This is a pioneer study of single nucleotide polymorphisms (SNPs) within the entire promoter region (1204 bp) dominant pPAG2-L subfamily in pig. The was sequenced/examined using genomic deoxyribonucleic acid (gDNA) templates crossbreed pigs (Landrace x Large White), and compared to two bacterial artificial chromosome (BAC) clones containing gDNA Duroc breed (as positive controls). Our analysis identified 31 SNPs one InDel mutation pigs. Among 42 BAC clones, 24 had not been previously...

Long non-coding RNAs (lncRNAs) are transcripts not translated into proteins with a length of more than 200 bp. LncRNAs considered an important factor in the regulation countless biological processes, mainly through gene expression and interactions proteins. However, detailed mechanism interaction as well functions lncRNAs still unclear therefore constitute serious research challenge. In this study, for first time, potential mechanisms lncRNA processes related to sperm motility turkey were...

The Eurasian beaver is one of the largest rodents that, despite its high impact on environment, a non-model species that lacks reference genome. Characterising genes critical for pregnancy outcome can serve as basis identifying mechanisms underlying effective reproduction, which required success endangered conservation programs. In present study, high-throughput RNA sequencing (RNA-seq) was used to analyse global changes in Castor fiber subplacenta transcriptome during multiple pregnancy. De...

This study presents pioneering data concerning the human pregnancy-associated glycoprotein-Like family, identified in genome, of term placental transcriptome and proteome. RNA-seq allowed identification 1364 bp hPAG-L/pep cDNA with at least 56.5% homology other aspartic proteinases (APs). In silico analyses revealed 388 amino acids (aa) full-length hPAG-L polypeptide precursor, 15 aa-signal peptide, 47 aa-blocking peptide 326 aa-mature protein, two Asp residues (D), specific for a catalytic...

Pulmonary embolism (PE) is a severe disease that usually originates from deep vein thrombosis (DVT) of the lower extremities. This study set out to investigate changes in transcriptome pulmonary artery (PA) course PE porcine model.The was performed on 11 male pigs: thrombus formed each right femoral six animals, and then released induce PE, remaining five animals served as control group. In experimental total RNA isolated PA where blood clot lodged, group, corresponding segments....

In silico identification of long noncoding RNAs (lncRNAs) is a multistage process including filtering transcripts according to their physical characteristics (e.g., length, exon–intron structure) and determination the coding potential sequence. A common issue within this choice most suitable method analysis for conducted research. Selection tools on sole basis single performance may not provide effective specific problem. To overcome these limitations, we developed R library lncRna, which...

Aspartic proteinases (AP) form a multigenic group widely distributed in various organisms and includes pepsins (pep), cathepsins D E, pregnancy associated glycoproteins (PAGs) as well plant, fungal, retroviral proteinases. This study describes the transcript identification expression localization of AP within discoid placenta Castor fiber. We identified 1257 bp cDNA sequence, encoding 391 amino acids (aa) polypeptide precursor composed 16 aa signal peptide, 46 pro-piece, 329 mature protein....

Our pioneering data provide the first comprehensive view of placental transcriptome beaver during single and multiple gestation. RNA-Seq a de novo approach allowed global pattern identification C. fiber transcriptome. Non-redundant comprised 211,802,336 nt transcripts, grouped into 128,459 contigs clustered 83,951 unigenes. An Ensembl database search revealed 14,487, 14,994, 15,004, 15,267 15,892 non-redundant homologs for Ictidomys tridecemlineatus, Rattus norvegicus, Mus musculus, Homo...