A5078477095- Botany and Plant Ecology Studies
- Bryophyte Studies and Records
- Genomics and Phylogenetic Studies
- Reproductive System and Pregnancy
- Plant Taxonomy and Phylogenetics
- Ovarian cancer diagnosis and treatment
- Extracellular vesicles in disease
- Estrogen and related hormone effects
- Pregnancy and preeclampsia studies
- Peatlands and Wetlands Ecology
- Lichen and fungal ecology
- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Toxic Organic Pollutants Impact
- Plant and Fungal Species Descriptions
- Effects and risks of endocrine disrupting chemicals
- Photosynthetic Processes and Mechanisms
- Microbial Community Ecology and Physiology
- Cardiac Imaging and Diagnostics
- Environmental Toxicology and Ecotoxicology
- Carcinogens and Genotoxicity Assessment
- RNA Research and Splicing
- Genetic diversity and population structure
- Parasite Biology and Host Interactions
- Ecology and biodiversity studies
Gdańsk Medical University
2022-2025
Polish Academy of Sciences
2020-2024
Institute of Animal Reproduction and Food Research
2020-2024
University of Gdańsk
2022
University of Warmia and Mazury in Olsztyn
2015-2021
National Institutes of Health
2020
Yale University
2020
Harvard University
2020
Rockefeller University
2020
Brigham and Women's Hospital
2020
Present study was designed to verify which or if any of plastome loci is a hotspot region for mutations and hence might be useful molecular species identification in feather grasses. 21 newly sequenced complete plastid genomes representing 19 taxa from the genus Stipa were analyzed search most variable discriminative within Stipa. The results showed that problem with selecting good barcode locus grasses lies very low level genetic diversity its plastome. None single chloroplast polymorphic...
Abstract Background Adipose-derived mesenchymal stromal cells (AD-MSCs) and fibroblasts are both widely used in regenerative medicine, demonstrating significant potential for personalized cell therapy. A major challenge their use lies high biological similarity, encompassing morphology, differentiation capabilities, flow cytometric markers, making distinction difficult. Methods In our study, we aimed to compare AD-MSCs obtained from two types of adipose tissue, subcutaneous visceral,...
Abstract Background Molecular research revealed that some of the European Calypogeia species described on basis morphological criteria are genetically heterogeneous and, in fact, complexes. DNA barcoding is already commonly used for correct identification difficult to determine species, disclose cryptic or detecting new taxa. Among liverworts, fragments, recommend as universal plant barcodes, cause problems amplification. Super-barcoding based genomic data, makes opportunities a...
Aneura pinguis is known as a species complex with several morphologically indiscernible species, which are often reproductively isolated from each other and show distinguishable genetic differences. Genetic dissimilarity of cryptic may be detected by genomes comparison. This study presents the first complete sequences chloroplast mitochondrial six A. complex: A, B, C, E, F, J. These have been compared to in order reconstruct phylogenetic relationships gain better understanding evolutionary...
Abstract A recently presented taxonomical arrangement of the moss genus Orthotrichum Hedw. s.l. substantially changed traditional view taxon that had been accepted throughout twentieth century. This paper provides results mitogenomic studies strongly support new concept. Comparative analyses in this study confirmed stable structure mitogenomes. Moreover, 17 complete mitogenome sequences were used to identify major evolutionary groups, including 11 newly sequenced ones, for study. The...
In early pregnancy, as the embryo arrives in uterus, intensive communication between and uterus begins. Hundreds of molecules are known to be involved, but despite numerous findings, full understanding complexity embryo–maternal dialog remains elusive. Recently, extracellular vesicles, nanoparticles able transfer functionally active cargo cells, have emerged important players cell–cell communication, such, they gained great attention over past decade also reproductive biology. Here, we use a...
Extracellular vesicles (EVs) are membrane-coated nanoparticles secreted by almost all cell types in living organisms. EVs, as paracrine mediators, involved intercellular communication, immune response, and several reproductive events, including the maintenance of pregnancy. Using a domestic animal model (Sus scrofa) with an epitheliochorial, superficial type placentation, we focused on EV biogenesis pathway at embryo-maternal interface, when embryonic signaling occurs for maternal...
Human skin is the natural source, place of metabolism, and target for vitamin D3. The classical active form D3, 1,25(OH)2D3, expresses pluripotent properties intensively studied in cancer prevention therapy. To define specific role D3 receptor (VDR) its co-receptor retinoid X alpha (RXRA) genomic regulation, VDR or RXRA genes were silenced squamous cell carcinoma line A431 treated with 1,25(OH)2D3 at long incubation time points 24 h/72 h. Extending WT (wild-type) cells resulted a two-fold...
The human placenta is a particular organ that inseparably binds the mother and fetus. proper development survival of conceptus relies on essential interplay between maternal fetal factors involved in cooperation within placenta. In our study, high-throughput sequencing (RNA-seq) was applied to analyze global transcriptome during uncomplicated pregnancies. RNA-seq utilized identify pattern gene expression placentas (N = 4) from women single twin During analyses, we obtained 228,044...
The mitogenomes of vascular plants are one the most structurally diverse molecules. In present study we characterize a rare and endangered species Pulsatilla patens. We investigated gene content its RNA editing potential, repeats distribution plastid derived sequences.The mitogenome structure early divergent eudicot, patens does not support master chromosome hypothesis, revealing presence three linear chromosomes total length 986 613 bp. molecules shaped by extremely long, exceeding 87 kbp...
How stimulation of the intestinal microbiota may regulate health-promoting effects dietary polyphenols on liver function has not been fully elucidated. Therefore, in this study, we examined whether a combination fructooligosaccharides (FOSs) and polyphenol preparation (PP) might stimulate to increase levels polyphenol-derived bioactive compounds. We also investigated how supplementation affected lipid metabolism antioxidative status Wistar rats without diet-induced metabolic disturbances....
Comparative analyses of chloroplast and mitochondrial genomes have shown that organelle in bryophytes evolve slowly. However, contrast to seed plants, the organellar are yet poorly explored bryophytes, especially among liverworts. Discovering another liverwort species by sequencing provides new conclusions on evolution bryophytes. In this work, Gymnomitrion concinnatum were sequenced, assembled annotated for first time. The genome displays, typical most quadripartite structure containing...
An active form of vitamin D3 (1,25-dihydroxyvitamin D3) acts through D receptor (VDR) initiating genomic response, but several studies described also non-genomic actions 1,25-dihydroxyvitamin D3, implying the role PDIA3 in process. is a membrane-associated disulfide isomerase involved bond formation, protein folding, and remodeling. Here, we used transcriptome-based approach to identify changes expression profiles PDIA3-deficient squamous cell carcinoma line A431 after treatment. knockout...
Lung cancer is responsible for the most cancer-related mortality worldwide and mechanism of its development poorly understood. Proteomics has become a powerful tool offering vital knowledge related to development. Using two-dimensional difference gel electrophoresis (2D-DIGE) approach, we sought compare tissue samples from non-small-cell lung (NSCLC) patients taken tumor center margin. Two subtypes NSCLC, adenocarcinoma (ADC) squamous cell carcinoma (SCC) were compared. Data are available...
Abstract Micronuclei (MN) can form through many mechanisms, including the breakage of aberrant cytokinetic chromatin bridges. The frequent observation MN in tumors suggests that they might not merely be passive elements but could instead play active roles tumor progression. Here, we propose a mechanism which presence micronuclei induce specific phenotypic and functional changes cells increase invasive potential cancer cells. Through integration diverse vitro imaging molecular techniques...
Liverwort mitogenomes are considered to be evolutionarily stable. A comparative analysis of four Calypogeia species revealed differences compared previously sequenced liverwort mitogenomes. Such involve unexpected structural changes in the two genes, cox1 and atp1, which have lost three introns, respectively. The group I introns gene proposed been by two-step localized retroprocessing, whereas one-step retroprocessing could responsible for disappearance II atp1 gene. These cases represent...
RNA editing alters the identity of nucleotides in an sequence so that mature transcript differs from template defined genome. This process has been observed chloroplasts and mitochondria both seed early land plants. However, frequency plant ranges zero to thousands sites. To date, analyses plants have conducted on a small number genes or mitochondrial genomes single species. study provides overview mitogenomic potential main lineages these two groups by predicting sites 33 37 species...
The chloroplast genomes of liverworts, an early land plant lineage, exhibit stable structure and gene content, however the known resources are very limited. newly sequenced plastomes Conocephalum, Riccia Sphaerocarpos species revealed increase simple sequence repeats during diversification complex thalloid liverwort lineage. presence long TA motifs forced applying long-read nanopore sequencing method for proper dependable plastome assembly, since length dinucleotide overcome Illumina short...
Molecular identification of species is especially important where traditional taxonomic methods fail. The genus Calypogeia belongs to one the tricky taxons. simple morphology these and a tendency towards environmental plasticity make them complicated in identification. finding universal single-locus DNA barcode plants seems be 'the Holy Grail'; therefore, researchers are increasingly looking for multiloci barcodes or super-barcoding. Since mitochondrial genome has low sequence variation...
The study presents results of research on the evolution plastid genomes in Stipa L. which is a large genus Poaceae family, comprising species diverse terms geographic distribution, growing under highly variated habitat conditions. Complete plastome sequences 43 taxa from Stipeae and Ampelodesmae tribes were analyzed for variability coding regions against background phylogenetic relationships within Stipa. hypothesis put forward our was that some are affected by selection pressure...
The structure of the Stipa lipskyi (GenBank accession no. KT692644) plastid genome is similar to that closely related Poaceae species: it has a total length 137 755 bp, base composition plastome following: A (30.7%), C (19.3%), G (19.4%) and T (30.5%). S. contains 71 genes, excluding second IR region. complete sequence will help development primers for examining phylogeny hybridization events in this taxonomically difficult genus.