A5050224198- Fibroblast Growth Factor Research
- Epigenetics and DNA Methylation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer Cells and Metastasis
- Estrogen and related hormone effects
- Kruppel-like factors research
- Pulmonary Hypertension Research and Treatments
- Cancer-related gene regulation
- Neonatal Respiratory Health Research
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Connective Tissue Growth Factor Research
- Immune Cell Function and Interaction
- Proteoglycans and glycosaminoglycans research
- Complement system in diseases
- Extracellular vesicles in disease
- Growth Hormone and Insulin-like Growth Factors
- Protein Tyrosine Phosphatases
- Prenatal Screening and Diagnostics
- Systemic Sclerosis and Related Diseases
- Cytokine Signaling Pathways and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Peptides and Activities
- BRCA gene mutations in cancer
- Ion channel regulation and function
Gdańsk Medical University
2011-2024
Justus-Liebig-Universität Gießen
2006-2009
Universities of Giessen and Marburg Lung Center
2009
Giessen School of Theology
2006-2007
Universitätsklinikum Gießen und Marburg
2007
Protein arginine methylation is catalyzed by a family of enzymes called protein methyltransferases (PRMTs). Three forms methylarginine have been identified in eukaryotes: monomethylarginine (l-NMMA), asymmetric dimethylarginine (ADMA), and symmetric (SDMA), all characterized one or both guanidine nitrogen atoms arginine. l-NMMA ADMA, but not SDMA, are competitive inhibitors nitric oxide synthase isoforms. SDMA eliminated almost entirely renal excretion, whereas ADMA further metabolized...
Lung fibrosis is characterized by increased deposition of ECM, especially collagens, and enhanced proliferation fibroblasts. l-arginine a key precursor nitric oxide, asymmetric dimethylarginine, proline, an amino acid enriched in collagen. We hypothesized that metabolism altered pulmonary fibrosis, ultimately affecting collagen synthesis. Expression analysis enzymes the arginine pathway, protein methyltransferases (Prmt), transporters, arginases quantitative (q) RT-PCR Western blot revealed...
Signaling mediated by growth factors receptors has long been suggested as one of the key responsible for failure endocrine treatment in breast cancer (BCa). Herein we present that presence tamoxifen, FGFs (Fibroblast Growth Factors) promote BCa cell with strongest effect being produced FGF7. FGFR2 was identified a mediator FGF7 action and FGFR2-induced signaling found to underlie cancer-associated fibroblasts-dependent resistance tamoxifen. FGF7/FGFR2-triggered pathway shown induce ER...
Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide synthesis. ADMA generated by catabolism proteins containing methylated arginine residues, and its levels are correlated with endothelial dysfunction in systemic cardiovascular diseases. Arginine methylation cellular catalyzed protein methyltransferases (PRMT). The expression localization PRMT the lung has not been addressed. Here, we sought to analyze isoforms determine whether altered during exposure chronic...
We have recently demonstrated that fibroblast growth factor receptor 2 (FGFR2)-mediated signalling alters progesterone (PR) activity and response of oestrogen α (ER)-positive (ER+) breast cancer (BCa) cell lines to anti-ER agents. Little is known about whether the crosstalk between ER PR, shown be modulated by hormonal background, might also affected FGFR2. Here, PR-dependent behaviour ER+ BCa cells was studied in presence (E2) (P4) and/or FGF7. In vitro analyses showed FGF7/FGFR2...
We have recently demonstrated that, fibroblast growth factor 2 (FGFR2), signalling via ribosomal S6 kinase (RSK2), promotes progression of breast cancer (BCa). Loss progesterone receptor (PR), whose activity in BCa cells can be stimulated by receptors (GFRs), is associated with poor patient outcome. Here we showed that FGF7/FGFR2 triggered phosphorylation PR at Ser294, ubiquitination and subsequent receptor`s degradation the 26S proteasome pathway cells. further RSK2 mediated...
Arginine methylation constitutes a posttranslational modification dependent on the action of protein arginine methyltransferases (PRMTs). Using S-adenosylmethionine as methyl donor, PRMTs catalyze formation monomethylarginine (L-NMMA), asymmetric dimethylarginine (ADMA), or symmetric (SDMA). Protein is involved in regulation signal transduction, RNA export, and cell proliferation, but quantitative view tissue proteome remains to be performed. In this study, we developed high-performance...
Abstract Background Genetic abnormalities in the FGFR signalling occur 40% of breast cancer (BCa) patients resistant to anti-ER therapy, which emphasizes potential FGFR-targeting strategies. Recent findings indicate that not only mutated is a driver tumour progression but co-mutational landscapes and other markers should be also investigated. Autophagy has been recognized as one major mechanisms underlying role microenvironment promotion cell survival, resistance drugs. The selective...
Deregulation of fibroblast growth factor receptors (FGFRs) signaling, as a result FGFR amplification, chromosomal translocation, or mutations, is involved in both initiation and progression wide range human cancers. Clinical data demonstrating the dependence cancer cells on FGFRs signaling clearly indicate these molecular targets anti-cancer therapies. Despite increasing number tyrosine kinase inhibitors (TKIs) being investigated clinical trials, acquired resistance to drugs poses serious...
FGFR signalling is one of the most prominent pathways involved in cell growth and development as well cancer progression. FGFR1 amplification occurs approximately 20% all squamous lung carcinomas (SCC), a predominant subtype non-small carcinoma (NSCLC), indicating potential target for new anti-cancer treatment. However, acquired resistance to this type therapies remains serious clinical challenge. Here, we investigated NSCLC lines response mechanism novel selective inhibitor CPL304110. We...
Abstract The role of fibroblast growth factor receptor 2 (FGFR2), an important mediator stromal paracrine and autocrine signals, in mammary gland morphogenesis breast cancer has been extensively studied over the last years. However, function FGFR2 signalling initiation epithelial oncogenic transformation remains elusive. Here, FGFR2-dependent behaviour nontumorigenic model cells was studied. In vitro analyses demonstrated that regulates cell communication with extracellular matrix (ECM)...
Preimplantation genetic diagnosis (PGD) remains nowadays a valid alternative for couples at high-risk of having child with disease and women older than 37-40 years the high risk chromosomal aneuploidies in embryos. However use PGD penetrance recessive, dominant X-liked disorders occurring early life is documented, debate exists regarding its appropriateness lower late-onset cancer susceptibility syndromes. The data efficacy different molecular techniques used are still lacking. We therefore...
35delG mutation in GJB2 gene is the most frequent genetic hearing loss. The carrier screening for to identify affected newborns at moment relatively inexpensive method deafness diagnosis. casual treatment of DFNB1 impossible. Preimplantation diagnosis (PGD) a allowing transfer free embryos and successful pregnancies. It's an established procedure research oocyte before fertilization or embryo implantation uterus.The aim present work was perform PGD couple who had already child with loss.The...
Antimicrobial peptides are part of the innate immune response, regulate inflammation and initiate acquired immunity. This study focused on theta-defensins that have been shown to anticancer properties.RTD-2 analogs were synthesized a peptide synthesizer. Cell viability was estimated using MTT test. Immunoprecipitation assay conducted determine molecular partner [Ser3,7,12,16]-RTD-2 analog.Here, we present biologically active analog selectively targets various types breast cancer cells....
Breast cancer (BCa) is the most common affecting women worldwide. Overexpression of human epidermal growth factor receptor 2 (HER2) occurs in ~20‑25% invasive ductal breast carcinomas and associated with more aggressive phenotype. Herceptin, a humanized antibody against HER2, standard therapy HER2‑overexpressing cases. Approximately one‑third patients relapse despite treatment. Therefore numerous studies have investigated molecular mechanisms Herceptin resistance. An interaction between HER2...
Einleitung: Idiopathic pulmonary fibrosis (IPF) is a devastating disease with no effective treatment options. The lungs of IPF patients are characterized by alveolar epithelial cell injury, an accumulation activated (myo)fibroblasts, and excessive deposition extracellular matrix, especially collagens. This is, to great extend, due enhanced TGF-beta activity. In this study, we hypothesized that distinct alterations in the expression activity receptors (TbR) its intracellular mediators, smads.
Rationale: Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by an increase of interstitial fibroblasts and excessive extracellular matrix deposition, in part mediated through enhanced TGF-β signaling. Epithelial-to-mesenchymal transition (EMT), the reversible phenotypic switching epithelial to fibroblast-like cells, has recently been proposed as causative factor tissue fibrosis, but its precise role lung remains be resolved. Methods: We analyzed whether EMT occurs...
Idiopathic pulmonary fibrosis (IPF) is a devastating disease, characterized by severe loss of respiratory function due to enhanced extracellular matrix (ECM) deposition and (myo)fibroblast proliferation. Repetitive epithelial injuries with impaired alveolar wound healing represent trigger mechanism for development fibrosis, but molecular mechanisms linking cell (AEC) repair are poorly understood. Using whole genome microarrays, we report altered gene expression profiles AEC derived from mice...
Fibrotic lung disease is characterized by distorted architecture and severe loss of respiratory function secondary to alveolar epithelial cell (AEC) hyperplasia, enhanced extracellular matrix (ECM) deposition fibroblast proliferation. Repetitive injuries with impaired wound healing altered AEC gene expression represent a trigger mechanism for development fibrosis. To reveal regulatory networks in fibrosis, we compared profiles freshly isolated obtained from mice 14 days after saline or...