A5102798194- Respiratory Support and Mechanisms
- Clinical Nutrition and Gastroenterology
- Pulmonary Hypertension Research and Treatments
- Fatty Acid Research and Health
- Mitochondrial Function and Pathology
- Transplantation: Methods and Outcomes
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Sepsis Diagnosis and Treatment
- Immune Response and Inflammation
- Appendicitis Diagnosis and Management
- Neonatal Respiratory Health Research
- Cardiac, Anesthesia and Surgical Outcomes
- Pleural and Pulmonary Diseases
- Mechanical Circulatory Support Devices
- COVID-19 Clinical Research Studies
- Abdominal Surgery and Complications
- Organ Transplantation Techniques and Outcomes
- Electrolyte and hormonal disorders
- Intensive Care Unit Cognitive Disorders
- Pancreatitis Pathology and Treatment
- Medical Imaging and Pathology Studies
- Case Reports on Hematomas
- Esophageal and GI Pathology
- Trauma and Emergency Care Studies
- Eicosanoids and Hypertension Pharmacology
German Center for Lung Research
2016-2025
Universities of Giessen and Marburg Lung Center
2016-2025
Justus-Liebig-Universität Gießen
2016-2025
Universitätsklinikum Gießen und Marburg
2015-2025
Cardio-Pulmonary Institute
2019-2024
Wayne State University
2017
Max Planck Institute for Heart and Lung Research
2017
Zero to Three
2017
RWTH Aachen University
2016
Saint Agnes Hospital
2016
Acute pulmonary oxygen sensing is essential to avoid life-threatening hypoxemia via hypoxic vasoconstriction (HPV) which matches perfusion ventilation. Hypoxia-induced mitochondrial superoxide release has been suggested as a critical step in the signaling pathway underlying HPV. However, identity of primary sensor and mechanism acute hypoxia, well its relevance for chronic sensing, remain unresolved.To investigate role pulmonary-specific isoform 2 subunit 4 complex IV (Cox4i2) subsequent...
Various forms of diffuse parenchymal lung disease have been proposed as potential consequences severe COVID‑19. We describe the clinical, radiological and histological findings patients with COVID‑19-associated acute respiratory distress syndrome who later developed organising pneumonia including longitudinal follow-up. Our may important implications for therapeutic modalities in late-phase COVID‑19 might partially explain why a subgroup benefits from systemic corticosteroids.
Abstract Background The SARS-CoV-2 pandemic is a major challenge for health care services worldwide. It’s impact on oncologic therapies and elective surgery has been described recently, the literature provides guidelines regarding appropriate patient treatment during pandemic. However, of emergency poorly investigated up to now. Methods A 17-item web survey had distributed surgeons in June 2020 around world, investigating patients septic diseases both requiring time-to-intervention routine,...
Background Electronic cigarette (e-cigarette) vapour is gaining popularity as an alternative to tobacco smoking and can induce acute lung injury. However, the specific role of nicotine in e-cigarette its long-term effects on airways, parenchyma vasculature remain unclear. Results In vitro exposure nicotine-containing extract (ECVE) or nicotine-free (NF ECVE) induced changes gene expression epithelial cells pulmonary arterial smooth muscle (PASMCs), but ECVE particular caused functional...
Mutations in the bmpr2 gene, encoding type II bone morphogenetic protein (BMP) receptor, have been identified patients with pulmonary arterial hypertension (PAH), implicating BMP signaling PAH. The aim of this study was to assess and its physiological effects a monocrotaline (MCT) model PAH.Expression receptors Ib II, Smads 4, 5, 6, 8, downregulated lungs but not kidneys MCT-treated rats. Smad1 phosphorylation expression BMP/Smad target genes id1 id3 also reduced, although ERK1/2 p38(MAPK)...
Background and Objectives Transmission of cytomegalovirus (CMV) to seronegative, immunocompromised recipients can cause serious fatal complications. Although the seroprevalence CMV is high, risk primary infection among healthy blood donors has not yet been analysed in a large population. Materials Methods We developed an algorithm determine rate seroconversion overall cohort 24 260 subjects who donated 176 474 units during 11‐year observation period. Results detected all relevant age groups...
<h3>Background:</h3> Idiopathic pulmonary fibrosis (IPF) is a fatal interstitial lung disease characterised by accumulation of activated (myo)fibroblasts and excessive extracellular matrix deposition. The enhanced may be attributed, in part, to the process transforming growth factor β1 (TGFβ1)-induced epithelial–mesenchymal transition (EMT), phenotypic switching epithelial fibroblast-like cells. Although alveolar type II (ATII) cells have been shown undergo EMT, precise mediators mechanisms...
Increased mitochondrial reactive oxygen species (ROS), particularly superoxide, have been suggested to mediate hypoxic pulmonary vasoconstriction (HPV), chronic hypoxia-induced hypertension and right ventricular remodelling. We determined ROS in acute hypoxia, investigated the effect of mitochondria-targeted antioxidant MitoQ under these conditions. The or its inactive carrier substance, decyltriphenylphosphonium, on HPV (1% O 2 for 10 min) was isolated blood-free perfused mouse lungs. Mice...
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Mitochondria play an important role in sensing both acute and chronic hypoxia the pulmonary vasculature, but their primary oxygen-sensing mechanism contribution to stabilization of hypoxia-inducible factor (HIF) remains elusive. Alteration mitochondrial electron flux increased superoxide release from complex III has been proposed as essential trigger for hypoxic vasoconstriction (HPV). We used mice expressing a tunicate alternative oxidase, AOX, which maintains when respiratory complexes...
Vascular smooth muscle cells (VSMCs) constitute the major cellular component of vessel tunica media. VSMC proliferation is a key feature in developing vessels and pathological states such as atherosclerosis restenosis. Transforming growth factor (TGF)-β regulator VSMCs, but its effect on apoptosis are controversial. Here, we characterized TGF-β effects basal-, serum-, platelet-derived factor-BB-induced primary mouse proliferation. led to potent inhibition VSMCs isolated from normal aortae...
Idiopathic pulmonary arterial hypertension (IPAH) is characterized by medial hypertrophy due to artery smooth muscle cell (paSMC) hyperplasia. Inflammation proposed play a role in vessel remodeling associated with IPAH. IL-13 emerging as regulator of tissue remodeling; however, the contribution system IPAH has not been assessed.The objective this study was assess possible IPAH.Expression and localization IL-13, receptors IL-4R, IL-13Rα1, IL-13Rα2 were assessed real-time reverse...
Numerous studies have described the immunosuppressive capacity of mesenchymal stem cells (MSC) but these use mixtures heterogeneous progenitor for in vitro expansion. Recently, multipotent MSC been prospectively identified murine bone marrow (BM) on basis PDFGRa+ SCA1+ CD45− TER119− (PαS) expression immunomodulatory is unknown. We isolated PαS by high-purity FACS sorting BM and after expansion we analyzed vivo activity during acute pneumonia. (1 × 106) were applied intratracheally 4 h...
Pulmonary arterial hypertension (PAH) can be found in patients suffering from a loss-of-function mutation of the gene encoding for activin receptor-like kinase 1 (ALK-1), bone morphogenetic protein (BMP) type receptor. Interestingly, ALK-1 mutations also lead to hereditary hemorrhagic telangiectasia (HHT), an autosomal dominant disease characterized by arteriovenous malformations (AVMs) leading potentially life-threatening bleeding complications such as epistaxis. Current therapeutic options...
Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is currently the major threat for immunocompromised individuals. The course of COVID-19 in lung transplant recipients Omicron era remains unknown. aim study was to assess outcome and associated factors a German-wide multicenter approach. All affected individuals from January 1st March 20th, 2022 8 German centers during wave were collected. Baseline characteristics antiviral...
Background Nucleated red blood cells (nRBC) are precursor of the erythropoiesis that absent from peripheral under physiological conditions. Their presence is associated with adverse outcomes in critically ill patients. This study aimed to evaluate predictive value nRBC on mortality intensive care unit (ICU) patients COVID-19 acute respiratory distress syndrome (ARDS). Material and methods retrospective, observational cohort analyzed data 206 ICU diagnosed ARDS between March 2020 2022. The...
Mutations in genes encoding members of the transforming growth factor (TGF)-β superfamily have been identified idiopathic forms pulmonary arterial hypertension (PAH). The current study examined whether perturbations to TGF-β/Smad2,3 signalling axis occurred a monocrotaline (MCT) rodent model experimental PAH. Expression TGF-β machinery was assessed lungs and kidneys MCT-treated rodents with severe PAH by semi-quantitative reverse-transcription (RT)-PCR, real-time RT-PCR immunoblotting....
Background— Pulmonary arterial hypertension (PAH) is characterized by selective elevation of pulmonary pressure. The pathological hallmark PAH the narrowing arterioles secondary to endothelial cell dysfunction and smooth muscle proliferation. Heterozygous mutations in BMPR2 , encoding type II bone morphogenetic protein receptor (BMPRII), were identified PAH, suggesting that alterations BMPRII function are involved disease onset and/or progression. Methods Results— We for activated C-kinase...
Acute lung injury (ALI) remains an important cause of mortality in intensive care units. Inflammation is controlled by cytokines and eicosanoids derived from the n-6 fatty acid (FA) arachidonic (AA). The n-3 FA eicosapentaenoic (EPA) docosahexaenoic (DHA) mediators EPA DHA possess reduced inflammatory potency.To determine whether ability fat-1 mice to endogenously convert FA, thus generate increased ratio impacts experimental ALI.We investigated ALI induced intratracheal instillation...