A5044732036- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Genetics and Neurodevelopmental Disorders
- Eosinophilic Disorders and Syndromes
- Autism Spectrum Disorder Research
- Multiple Myeloma Research and Treatments
- Cancer-related Molecular Pathways
- Lymphoma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Trace Elements in Health
- Protein Degradation and Inhibitors
- Cancer Cells and Metastasis
- Immunodeficiency and Autoimmune Disorders
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Mast cells and histamine
- Explainable Artificial Intelligence (XAI)
Philipps University of Marburg
2015-2025
Universitätsklinikum Gießen und Marburg
2015-2024
Universität Ulm
2010-2017
National Tsing Hua University
2017
Max Planck Institute for Polymer Research
2017
Justus-Liebig-Universität Gießen
2003-2014
Universitätsmedizin Göttingen
2014
Institute of Molecular Biology
2014
Neurologisches Rehabilitationszentrum Leipzig
2014
Leipzig University
2014
Although the development of tyrosine kinase inhibitors (TKIs) to control unregulated activity BCR-ABL revolutionized therapy chronic myeloid leukemia, resistance TKIs is a clinical reality. Among postulated mechanisms overexpression ATP-binding cassette (ABC) transporters, such as P-glycoprotein (ABCB1) and breast cancer protein (ABCG2), which mediate reduced intracellular drug accumulation. We compared interactions imatinib, nilotinib, dasatinib with ABCB1 ABCG2 in ex vivo vitro systems....
Follicular T-helper (T FH ) cells cooperate with GL7 + CD95 germinal center (GC) B to induce antibody maturation. Herein, we identify the transcription factor IRF4 as a T-cell intrinsic precondition for T cell differentiation and GC formation. After immunization protein or infection protozoon Leishmania major , draining lymph nodes (LNs) of IFN-regulatory factor-4 ( Irf4 −/− mice lacked GCs despite developing normal initial hyperplasia. were also absent in Peyer’s patches naive mice....
Rett syndrome (RTT) is an inborn neurodevelopmental disorder caused by mutations in the X-linked methyl-CpG binding protein 2 gene (MECP2). Besides mental retardation, most patients suffer from potentially life-threatening breathing arrhythmia. To study its pathophysiology, we performed comparative analyses of phenotype Mecp2-/y knockout (KO) and C57BL/6J wild-type mice using perfused working heart-brainstem preparation (WHBP). We simultaneously recorded phrenic efferent vagal nerve...
Thirty-five percent of patients with Rett syndrome carry nonsense mutations in the MECP2 gene. We have recently shown transfected HeLa cells that readthrough gene can be achieved by treatment gentamicin and geneticin. This study was performed to test if also endogenously expressing mutant MeCP2 evaluate potentially more effective compounds. A mouse model generated carrying R168X mutation Transfected mutated fusion proteins ear fibroblasts isolated from new were treated novel aminoglycosides...
Introduction Inflammatory conditions in patients have various causes and require different treatments. Bacterial infections are treated with antibiotics, while these medications ineffective against viral infections. Autoimmune diseases graft-versus-host disease (GVHD) after allogeneic stem cell transplantation, immunosuppressive therapies such as glucocorticoids, which may be contraindicated other inflammatory states. In this study, we employ a combination of straightforward blood tests to...
Numerous studies have described the immunosuppressive capacity of mesenchymal stem cells (MSC) but these use mixtures heterogeneous progenitor for in vitro expansion. Recently, multipotent MSC been prospectively identified murine bone marrow (BM) on basis PDFGRa+ SCA1+ CD45− TER119− (PαS) expression immunomodulatory is unknown. We isolated PαS by high-purity FACS sorting BM and after expansion we analyzed vivo activity during acute pneumonia. (1 × 106) were applied intratracheally 4 h...
Rett syndrome, one of the most common causes mental retardation in females, is caused by mutations X chromosomal gene MECP2. Mice deficient for MeCP2 recapitulate some symptoms seen patients with syndrome. It has been shown that reactivation silent MECP2 alleles can reverse these mice. We have generated a knockin mouse model translational research carries nonsense mutation R168X. In this article we describe phenotype model. male MeCP2R168X mice life span was reduced to 12–14 weeks and...
Mesenchymal stem cells (MSCs) have entered the clinic as an Advanced Therapy Medicinal Product and are currently evaluated in a wide range of studies for tissue regeneration or autoimmune disorders. Various efforts been made to standardize optimize expansion manufacturing processes, but until now reliable potency assays final MSC product lacking. Because recent findings suggest superior therapeutic efficacy freshly administered MSCs comparison with frozen cells, we sought correlate T-cell...
Differential induction therapy of all subtypes acute myeloid leukemia other than promyelocytic is impeded by the long time required to complete complex and diverse cytogenetic molecular genetic analyses for risk stratification or targeted treatment decisions. Here, we describe a reliable, rapid sensitive diagnostic approach that combines karyotyping mutational screening in single, integrated, next-generation sequencing assay. Numerical was performed low coverage whole genome followed copy...
Broad differentiation capacity has been described for mesenchymal stem cells (MSC) from human bone marrow. We sought to identify genes associated with the immature state and pluripotency of this cell type. To prove pluripotent MSC, into osteocytes, adipocytes, chondrocytes was performed in vitro. In contrast, normal skin did not harbor these abilities. compared expression profile marrow MSC cDNA one primary line as control, using a chip providing 9600 genes. The identity all relevant...
Rett syndrome, a common cause of mental retardation in females, is caused by mutations the MECP2 gene. Most females with fulfil established clinical criteria for but single cases asymptomatic carriers have been described. It therefore likely that there are individuals falling between these two extreme phenotypes.To describe three patients showing only minor symptoms syndrome.The patient best intellectual ability had predominantly psychiatric problems episodes uncontrolled aggression not...
Mesenchymal stromal cells (MSCs) have an inherent migratory capacity towards tumor tissue in vivo. With the future objective to quantify homing efficacy of MSCs, as first step this direction we investigated use inorganic nanoparticles (NPs), particular ca. 4 nm-sized Au NPs, for MSC labeling. Time dependent uptake efficiencies NPs at different exposure concentrations and times were determined via inductively coupled plasma mass spectrometry (ICP-MS). The labeling efficiency MSCs was terms...