A5091742786- Chronic Obstructive Pulmonary Disease (COPD) Research
- Neonatal Respiratory Health Research
- Single-cell and spatial transcriptomics
- Mycotoxins in Agriculture and Food
- Cell Image Analysis Techniques
- Congenital Diaphragmatic Hernia Studies
- Antifungal resistance and susceptibility
- Pulmonary Hypertension Research and Treatments
- Molecular Biology Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Hedgehog Signaling Pathway Studies
- Respiratory Support and Mechanisms
- RNA Research and Splicing
- Tumors and Oncological Cases
- Inhalation and Respiratory Drug Delivery
- Neuroscience of respiration and sleep
- Rabies epidemiology and control
- Parasitic Diseases Research and Treatment
- Systemic Sclerosis and Related Diseases
- Medical Imaging and Pathology Studies
- Nitric Oxide and Endothelin Effects
- Biosensors and Analytical Detection
- Head and Neck Surgical Oncology
- 3D Printing in Biomedical Research
- Cystic Fibrosis Research Advances
Universities of Giessen and Marburg Lung Center
2013-2023
Justus-Liebig-Universität Gießen
2013-2023
German Center for Lung Research
2014-2022
Cardio-Pulmonary Institute
2020-2022
Université Toulouse III - Paul Sabatier
2012
Toxalim Research Centre in Food Toxicology
2011
École Nationale Vétérinaire de Toulouse
2009
Inhalation of Aspergillus fumigatus conidia can cause severe aspergillosis in immunosuppressed people. A. produces a large number secondary metabolites, some which are airborne by and whose toxicity to the respiratory tract has not been investigated. We found that spores contain five main compounds, tryptoquivaline F, fumiquinazoline C, questin, monomethylsulochrin trypacidin. Fractionation culture extracts using RP-HPLC LC-MS showed samples containing trypacidin were toxic human A549 lung...
Chronic obstructive pulmonary disease (COPD) is a major cause of death worldwide. No therapy stopping progress the available.To investigate role soluble guanylate cyclase (sGC)-cGMP axis in development lung emphysema and hypertension (PH) to test whether sGC-cGMP treatment target for these conditions.Investigations were performed human tissue from patients with COPD, healthy donors, mice, guinea pigs. Mice exposed cigarette smoke (CS) 6 hours per day, 5 days week up months treated BAY...
Increased mitochondrial reactive oxygen species (ROS), particularly superoxide, have been suggested to mediate hypoxic pulmonary vasoconstriction (HPV), chronic hypoxia-induced hypertension and right ventricular remodelling. We determined ROS in acute hypoxia, investigated the effect of mitochondria-targeted antioxidant MitoQ under these conditions. The or its inactive carrier substance, decyltriphenylphosphonium, on HPV (1% O 2 for 10 min) was isolated blood-free perfused mouse lungs. Mice...
Changes in cell identities and positions underlie tissue development disease progression. Although single-cell mRNA sequencing (scRNA-Seq) methods rapidly generate extensive lists of states, spatially resolved mapping presents a challenging task. We developed SCRINSHOT (Single-Cell Resolution IN Situ Hybridization On Tissues), sensitive, multiplex RNA approach. Direct hybridization padlock probes on is followed by circularization with SplintR ligase rolling circle amplification (RCA) the...
Chronic obstructive pulmonary disease, encompassing chronic airway obstruction and lung emphysema, is a major worldwide health problem severe socio-economic burden. Evidence previously provided by our group has shown that inhibition of inducible NOS (iNOS) prevents development mild emphysema in mouse model tobacco smoke exposure can even trigger regeneration. Moreover, we could demonstrate hypertension not only abolished cigarette smoke-exposed iNOS-/- mice but also precedes development....
Inhaled therapeutics have high potential for the treatment of chronic respiratory diseases unmet medical need, such as idiopathic pulmonary fibrosis (IPF). Preclinical and early clinical evidence show that cellular communication network factor 2 (CCN2), previously called connective tissue growth (CTGF), is a promising target IPF. In recent phase 3 trials, however, systemic CCN2 inhibition failed to demonstrate clinically meaningful benefit. Here, we present preclinical profile inhaled...
Summary Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality worldwide. COPD caused by chronic exposure to cigarette smoke and/or other environmental pollutants that are believed induce reactive oxygen species (ROS) gradually disrupt signalling pathways responsible for maintaining lung integrity. Here we identify the antioxidant protein sestrin-2 (SESN2) as repressor PDGFRβ signalling, an upstream regulator alveolar maintenance programmes. In mice,...
ABSTRACT The tracheal epithelium is a primary target for pulmonary diseases as it provides conduit air flow between the environment and lung lobes. cellular molecular mechanisms underlying airway epithelial cell proliferation differentiation remain poorly understood. Hedgehog (HH) signaling orchestrates communication mesenchymal cells in lung, where modulates stromal proliferation, back to epithelium. Here, we reveal previously unreported autocrine function of HH cells. Epithelial depletion...
A hallmark of acute respiratory distress syndrome (ARDS) is an accumulation protein-rich alveolar edema that impairs gas exchange and leads to worse outcomes. Thus, understanding the mechanisms albumin clearance high clinical relevance. Here, we investigated cellular uptake in a three-dimensional culture precision-cut lung slices (PCLS). We found up 60% PCLS cells incorporated labeled time- concentration-dependent manner, whereas virtually no dextran was observed. Of note, at low temperature...
Mucus hypersecretion and mucus obstruction are pathogenic features in many chronic lung diseases directly linked to disease severity, exacerbation, progression, mortality. The Jagged-1/Notch pathway is a promising therapeutic target that regulates secretory ciliated cell trans-differentiation the lung. However, Notch also required various other organs. Hence, pulmonary delivery of agents approach this while minimizing systemic exposure. Using Anticalin® technology, Jagged-1 Anticalin binding...
A controlled clinical trial was carried out to assess the effectiveness of pyriprole, metaflumizone combined with amitraz, and fipronil‐(S)‐methoprene commercial spot‐on products in preventing adult female Culex pipiens from feeding on dogs. Twenty‐four beagle dogs were tested for their attractiveness mosquitoes ranked accordingly produce four groups equivalent sensitivity mosquitoes; six treated pyriprole spot‐on, amitraz left untreated. All challenged 50 unfed cages one hour seven days...
Abstract Changes in cell identities and positions underlie tissue development disease progression. Although, single-cell mRNA sequencing (scRNA-Seq) methods rapidly generate extensive lists of cell-states, spatially resolved mapping presents a challenging task. We developed SCRINSHOT ( S ingle C ell R esolution IN itu H ybridization O n T issues), sensitive, multiplex RNA approach. Direct hybridization padlock probes on is followed by circularization with SplintR ligase rolling circle...
Chronic obstructive pulmonary disease (COPD) is a major cause of death and disability worldwide. An estimated portion 30 – 70% COPD patients also suffer from hypertension (PH). Studies indicate that activation serotonin-mediated pathways contribute to development PH. Moreover, vascular alterations have been suggested emphysema development.
Abstract Secretory cells are major structural and functional constituents of the lung airways. Their spatial organization specification mechanisms partially understood. Here, we labelled secretory airway cell types analysed them at single-cell resolution. We found opposing, overlapping gene-expression gradients along proximal-distal axis superimposed on a general gene program encoding detoxification. One graded is elevated proximally relates to innate immunity, whereas other enriched...
In the murine airway epithelium, basal cells function as stem/progenitor that can both self-renew and produce differentiated secretory ciliated cells. Secretory also act progenitor club Interestingly, these are composed of different subpopulations: BASC (bronchio-alveolar stem cells), naphthalene-resistant (variant non-naphthalene resistant subpopulation. The epithelium mature lung differs in cellular composition, structural organization along proximal-distal axis. Based on single-cell...