A5060335517- Neonatal Respiratory Health Research
- Ion Transport and Channel Regulation
- Pancreatic function and diabetes
- Cardiovascular Function and Risk Factors
- Neonatal Health and Biochemistry
- Respiratory Support and Mechanisms
- Erythrocyte Function and Pathophysiology
- Liver Disease Diagnosis and Treatment
- Pulmonary Hypertension Research and Treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Congenital Diaphragmatic Hernia Studies
- Membrane-based Ion Separation Techniques
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Monoclonal and Polyclonal Antibodies Research
- Genetics and Neurodevelopmental Disorders
- Medical and Biological Ozone Research
- Human Health and Disease
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Lung Cancer Research Studies
- Tracheal and airway disorders
- Neuroscience of respiration and sleep
- Renal function and acid-base balance
Justus-Liebig-Universität Gießen
2019-2024
German Center for Lung Research
2018-2024
Universities of Giessen and Marburg Lung Center
2018-2024
Cardio-Pulmonary Institute
2019-2024
Institut für Lungenforschung
2024
Zaporizhia State Medical and Pharmaceutical University
2012
Acute respiratory distress syndrome (ARDS) is associated with high morbidity and mortality but lacks specific therapeutic options. Diverse endocytic processes play a key role in all phases of acute lung injury (ALI), including the initial insult, development failure due to alveolar flooding, as consequence altered alveolar-capillary barrier function, well resolution or deleterious remodeling after injury. In particular, clathrin-, caveolae-, endophilin- glycosylphosphatidyl inositol-anchored...
Persistent symptoms and radiographic abnormalities suggestive of failed lung repair are among the most common in patients with COVID-19 after hospital discharge. In mechanically ventilated acute respiratory distress syndrome (ARDS) secondary to SARS-CoV-2 pneumonia, low tidal volumes reduce ventilator-induced injury necessarily elevate blood CO2 levels, often leading hypercapnia. The role hypercapnia on is not completely understood. Here - using a mouse model exposure, cell lineage tracing,...
A hallmark of acute respiratory distress syndrome (ARDS) is an accumulation protein-rich alveolar edema that impairs gas exchange and leads to worse outcomes. Thus, understanding the mechanisms albumin clearance high clinical relevance. Here, we investigated cellular uptake in a three-dimensional culture precision-cut lung slices (PCLS). We found up 60% PCLS cells incorporated labeled time- concentration-dependent manner, whereas virtually no dextran was observed. Of note, at low temperature...
Abstract The Na,K-ATPase establishes the electrochemical gradient of cells by driving an active exchange Na + and K ions while consuming ATP. minimal functional transporter consists a catalytic α-subunit β-subunit with chaperon activity. also functions as cell adhesion molecule participates in various intracellular signaling pathways. maturation trafficking include co- post-translational processing enzyme endoplasmic reticulum (ER) Golgi apparatus subsequent delivery to plasma membrane (PM)....
Alveolar edema, impaired alveolar fluid clearance, and elevated CO2 levels (hypercapnia) are hallmarks of the acute respiratory distress syndrome (ARDS). This study investigated how hypercapnia affects maturation Na,K-ATPase (NKA), a key membrane transporter, cell adhesion molecule involved in resolution edema endoplasmic reticulum (ER). Exposure human epithelial cells to concentrations caused significant retention NKA-β ER and, thus, decreased transporter Golgi apparatus. These effects were...
Acute respiratory distress syndrome (ARDS) is a common complication of influenza virus (IV) infection. During ARDS, alveolar protein concentrations often reach 40-90% plasma levels, causing severe impairment gas exchange and promoting deleterious remodeling. Protein clearance from the space at least in part facilitated by multi-ligand receptor megalin through clathrin-mediated endocytosis.To investigate whether IV infection impairs clearance, we examined albumin uptake expression MLE-12...
Acute respiratory distress syndrome is often associated with elevated levels of CO2 (hypercapnia) and impaired alveolar fluid clearance. Misfolding the Na,K-ATPase (NKA), a key molecule involved in both epithelial barrier tightness resolution edema, endoplasmic reticulum (ER) may decrease plasma membrane abundance transporter. Here, we investigated how hypercapnia affects NKA β-subunit (NKA-β) ER. Exposing murine precision-cut lung slices human A549 cells to led rapid NKA-β ER at cell...
Several acute and chronic lung diseases are associated with alveolar hypoventilation leading to accumulation of CO 2 (hypercapnia). The β-subunit the Na,K-ATPase plays a pivotal role in maintaining epithelial integrity by functioning as cell adhesion molecule regulating surface stability catalytic α-subunit transporter, thereby, optimal fluid balance. Here, we identified E3 ubiquitin ligase for β-subunit, which promoted polyubiquitination, subsequent endocytosis proteasomal degradation...
Aims: Hepatic T1-time derived from cardiac magnetic resonance imaging (cMRI) reflects venous congestion and may provide a simple alternative to invasive end-diastolic elastance (Eed) for assessment of right ventricular (RV) diastolic function. We investigated the association native hepatic with single-beat Eed value longitudinal monitoring in pulmonary hypertension (PH).Methods results: retrospectively enrolled 85 patients suspected PH (59% female; 78 diagnosed; 7 excluded) who underwent...
Elevated carbon dioxide levels (hypercapnia) are frequently observed in patients with acute and chronic lung diseases. Na,K-ATPase is a sodium transporter junctional molecule that critically required for the maintenance of optimal alveolar fluid balance thus gas exchange. Here, we explored how hypercapnia affects maturation endoplasmic reticulum (ER). Murine precision-cut slices human epithelial cells exposed to elevated CO<sub>2</sub> showed rapid dose-dependent reduction β-subunit ER...
Elevated levels of CO<sub>2</sub> (hypercapnia) are often observed in patients with chronic obstructive pulmonary disease (COPD). Recently it has been shown that increased CO<sub>2</sub>, by activating intracellular calcium signalling pathways, might lead to airway obstruction a preclinical setting. Here, we investigate whether hypercapnia influences resistance COPD. We retrospectively analysed clinical characteristics, blood gases and lung function parameters 84 COPD GOLD stage 2 – 4, who...
Elevated levels of CO2 (hypercapnia) can be often detected in patients with acute and chronic lung diseases. Protein translation is an integral part protein synthesis which a major determinant cellular expression plays critical role maintaining homeostasis. In the present study, we investigated how hypercapnia effects on translation.
ABSTRACT Persistent symptoms and radiographic abnormalities suggestive of failed lung repair are among the most common in patients with COVID-19 after hospital discharge. In mechanically ventilated ARDS secondary to SARS-CoV-2 pneumonia, low tidal volumes reduce ventilator-induced injury necessarily elevate blood CO 2 levels, often leading hypercapnia. The role hypercapnia on is not completely understood. Here, we show that limits β-catenin signaling alveolar type (AT2) cells, reduced...