Postreperfusion syndrome (PRS) is a state of significant hemodynamic instability following graft reperfusion during orthotopic liver transplantation (OLTx). We aimed to investigate its risk factors and influence on patient outcome based single centre's experience.A retrospective study group 340 patients undergoing OLTx the period 2005-2008 was conducted. Piggy-back technique employed in 266 cases classical 64. PRS defined as decrease mean arterial pressure greater than 30% below baseline for...

Cellobiose dehydrogenase (CDH) is an extracellular hemoflavoprotein catalyzing the oxidation reaction of β-1,4-glycosidic-bonded sugars (lactose or cellobiose), which results in formation aldobionic acids and hydrogen peroxide as a byproduct. The biotechnological application CDH requires immobilization enzyme on suitable support. As carrier natural origin used for immobilization, chitosan seems to increase catalytic potential enzyme, especially applications packaging food industry dressing...

Human skin is the natural source, place of metabolism, and target for vitamin D3. The classical active form D3, 1,25(OH)2D3, expresses pluripotent properties intensively studied in cancer prevention therapy. To define specific role D3 receptor (VDR) its co-receptor retinoid X alpha (RXRA) genomic regulation, VDR or RXRA genes were silenced squamous cell carcinoma line A431 treated with 1,25(OH)2D3 at long incubation time points 24 h/72 h. Extending WT (wild-type) cells resulted a two-fold...

The aim of the study was to investigate mixtures comprising corn distillers dried grain with solubles as a partial replacer for soybean meal (SBM) and different dietary fat sources, in order determine their effect on meat quality fatty acid profile. Thirty-two crossbred fatteners were divided into four groups: I–SBM + rapeseed oil, II–cDDGS III–cDDGS beef tallow, IV–cDDGS coconut oil. experiment took place from 60 118 kg. At end fattening, all pigs slaughtered samples (musculuslongissimus...

An active form of vitamin D3 (1,25-dihydroxyvitamin D3) acts through D receptor (VDR) initiating genomic response, but several studies described also non-genomic actions 1,25-dihydroxyvitamin D3, implying the role PDIA3 in process. is a membrane-associated disulfide isomerase involved bond formation, protein folding, and remodeling. Here, we used transcriptome-based approach to identify changes expression profiles PDIA3-deficient squamous cell carcinoma line A431 after treatment. knockout...

Interleukin 33 (IL-33) belongs to the IL-1 family and is produced constitutively by epithelial endothelial cells of various organs, such as skin. It takes part in maintenance tissue homeostasis, repair, immune response, including activation Th2 lymphocytes. Its involvement pathogenesis several inflammatory diseases psoriasis was also suggested, but this not fully understood. The aim study investigate expression IL-33 its receptor, ST2, psoriasis, effects active form vitamin D (1,25(OH)2D3)...

Deregulation of mitochondria activity is one the hallmarks cancerogenesis and an important target for cancer therapy. Therefore, we compared impact active form vitamin D3 (1,25(OH)2D3) on mitochondrial morphology bioenergetics in human squamous cell carcinoma (A431) immortalized HaCaT keratinocytes. It was shown that cancerous A431 cells differ from observed keratinocytes terms network, morphology, bioenergetics, glycolysis, DNA copy number, while treatment with 1,25(OH)2D3 partially...

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The genomic activity of 1,25(OH)2D3 is mediated by vitamin D receptor (VDR), whilst non-genomic associated with protein disulfide isomerase family A member 3 (PDIA3). Interestingly, our recent studies documented that PDIA3 also involved, directly or indirectly, in the modulation response to 1,25(OH)2D3. Moreover, was shown regulate cellular bioenergetics, possibly through STAT signaling. Here, role VDR and proteins membrane calcium signaling investigated squamous cell carcinoma A431 line...

Background. Hypertension-induced endothelial dysfunction is associated with an impaired bioavailability of nitric oxide regulated through interactions between synthase and heat shock protein-90 (Hsp-90). The role Hsp-90 in the development arterial hypertension remains unclear.Objectives. objective study was to evaluate serum concentrations Hsp-90a patients comparison their normotensive counterparts.Material Methods. performed on 49 adults (mean age 55.6 years) elevated waist circumference....

Electric cell–substrate impedance sensing is an advanced in vitro measuring system which uses alternating current to determine behavior of cells physiological conditions. In this study, we used the abovementioned method for checking anticancer activities betulin and betulinic acid, are some most commonly found triterpenes nature. our experiment, threshold concentrations required elicit antiproliferative effects, verified by MTT LDH release methods, were 7.8 µM breast cancer (T47D), 9.5 lung...

Abstract The effect of corn distillers dried grains with solubles (DDGS), used in grower-finisher diets or without supplemental enzymes, on growth performance and carcass meat quality was determined an experiment 48 pigs. Group I (control) received a standard grain-soybean meal mixture DDGS enzymes; II fed containing 15% (grower) 20% (finisher) but the III NSP-hydrolyzing enzymes (200 g t-1). feed mixtures were isonitrogenous isoenergetic. All pigs fattened from 30 to 112 kg. At end all...

Patch clamp recordings carried out in the inside-out configuration revealed activity of three kinds channels: nonselective cation channels, small-conductance K(+) and large-conductance anion channels. The channels did not distinguish between Na(+) K(+). unitary conductance these reached 28 pS a symmetrical concentration 200 mM NaCl. A lower value this parameter was recorded for 50-fold gradient (200 mM/4 mM) it 8 pS. high selectivity to potassium confirmed by reversal potential (-97 mV),...

Abstract PDIA3 is an endoplasmic reticulum disulfide isomerase which involved in the folding and trafficking of newly synthesized proteins. was also described as alternative receptor for active form vitamin D 1,25(OH) 2 3 . Here, we investigated impact mitochondrial morphology bioenergetics squamous cell carcinoma line A431 treated with It observed that deletion resulted changes mitochondria including a decrease percentage section area, maximal diameter, perimeter. treatment A431∆ cells...