A5033602569- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Prenatal Screening and Diagnostics
- Neonatal Respiratory Health Research
- Gestational Diabetes Research and Management
- Epigenetics and DNA Methylation
- Pregnancy-related medical research
- Genetic Syndromes and Imprinting
- Growth Hormone and Insulin-like Growth Factors
- MicroRNA in disease regulation
- Reproductive Biology and Fertility
- Angiogenesis and VEGF in Cancer
- Animal Genetics and Reproduction
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Agriculture and Farm Safety
- Infant Nutrition and Health
- Cancer-related gene regulation
- Metabolism and Genetic Disorders
- Gestational Trophoblastic Disease Studies
- Genetic and phenotypic traits in livestock
- Occupational Health and Performance
- Circular RNAs in diseases
Colorado State University
2016-2025
Clemson University
2023
University of Malaya
2023
Eisenhower Medical Center
2012
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2012
National Institutes of Health
2012
Center for Scientific Review
2012
Ottawa Hospital
2012
Ottawa Hospital Research Institute
2012
Reproductive Science Center
2012
Pregnancy in cattle and sheep can be diagnosed by the presence of a conceptus-derived antigen maternal serum that is secreted trophoblast placental tissue primarily as an acidic component Mr 67,000. Molecular cloning its cDNA reveals belongs to aspartic proteinase family has greater than 50% amino acid sequence identity pepsin, cathepsin D, E. The inferred sequences ovine bovine polypeptides show approximately 73% each other. Critical substitutions at active site regions suggest both...
The ruminant conceptus synthesizes and secretes interferon (IFN)-τ, which presumably acts via an intrauterine paracrine mechanism to signal maternal recognition of pregnancy. aims this study were determine whether IFN-stimulated genes (ISG) such as ISG15 OAS-1 are differentially expressed in blood cells circulating the uterus ewes; extrauterine components reproductive tract corpus luteum (CL) also express mRNA for these ISG, antiviral activity is greater uterine vein than artery during early...
Bovine trophoblast protein-1 (bTP-1) is a secreted glycoprotein that consists of several forms differing slightly in mol wt and isoelectric point. It produced by bovine conceptuses after about day 15 pregnancy believed to play key role signalling the presence an embryo mother. In this study, series recombinant cDNA clones corresponding mRNA for bTP-1 have been isolated from libraries representing 18–19 conceptus poly(A)+ mRNA. Base sequencing cDNAs indicated multiple mRNAs exist. Northern...
Interferon tau (IFNT) from the ovine conceptus has paracrine actions on endometrium that alter release of prostaglandin F(2alpha) (PGF) and protect corpus luteum (CL). Antiviral activity in uterine vein blood expression interferon-stimulated genes (ISGs) CL is greater pregnant than nonpregnant ewes. We hypothesized IFNT contributes to antiviral endocrine CL. Preadsorption with antiserum against recombinant (ro) revealed ewes was mediated by IFNT. Endocrine were examined after infusing either...
Placental growth factor (PlGF) and vascular endothelial (VEGF) are involved in placental angiogenesis through interactions with the VEGFR-1 VEGFR-2 receptors. The placenta of pregnancies whose outcome is fetal restriction (FGR) characterized by abnormal angiogenic development, classically associated hypoxia. present study evaluated near-term expression this family an ovine model insufficiency-FGR, relationship to uteroplacental oxygenation. Compared controls, FGR demonstrated a 37% increase...
Summary. This paper demonstrates that a group of proteins, representing major secretory component cattle conceptuses, is immunologically related to ovine trophoblast protein-1 (oTP-1), principal product culture Day 13 21 sheep conceptuses. Conceptuses from cows (Day 17–18) and ewes 16–17) were cultured for 24 h in the presence l-|3H]leucine. By using rabbit antiserum oTP-1 Ouchterlony double-immunodiffusion analysis it was shown material bovine conceptus medium serologically related, but not...
Gonadal differentiation in the mammalian fetus involves a complex dose-dependent genetic network. Initiation and progression of fetal ovarian testicular pathways are accompanied by dynamic expression patterns thousands genes. We postulate these regulated small non-coding RNAs called microRNAs (miRNAs). The aim this study was to identify miRNAs gonads using sheep as model.We determined 128 real time PCR early-gestational (gestational day (GD) 42) mid-gestational (GD75) ovaries testes....
The antiviral activity of interferon (IFN) increases in uterine vein serum (UVS) during early pregnancy sheep. This UVS collected on Day 15 is blocked by anti-IFN-tau (anti-IFNT) antibodies. Conceptus-derived IFNT was hypothesized to induce IFN-stimulated gene (ISG) expression endometrium and extrauterine tissues pregnancy. To test this hypothesis, blood from ewes Days 12-16 the estrous cycle or Serum progesterone >1.7 ng/ml pregnant (P) nonpregnant (NP) until 13, then declined <0.6 NP ewes....
Ovine trophoblast protein-1 (oTP-1), an interferon of embryonic origin, is produced during the peri-implantation period early pregnancy. Secretion oTP-1 detectable between days 13 and 21, but not beyond. In this study, levels mRNA in embryos were analyzed to determine if they reflected transient nature production. Total cellular RNA (tcRNA) was isolated from representing day 12 (n = 5), 14 7), 16 18 6), 20 4), 22 5) pregnancy spotted on nylon membranes. Complementary transcribed a specific...
In normal pregnancy, cortisol increases; however, further pathological increases in are associated with maternal and fetal morbidities. These experiments were designed to test the hypothesis that increased would increase glucose concentrations, suppress growth, impair neonatal homeostasis. Ewes infused (1 mg·kg(-1)·day(-1)) from day 115 of gestation term; glucose, insulin, ovine placental lactogen, estrone, progesterone, nonesterified free fatty acids (NEFA), β-hydroxybutyrate (BHB),...
Ovine placental lactogen (oPL) is structurally similar to PRL, a product of the chorionic epithelium, and has been implicated in playing supportive role fetal growth. This study examined concentration cellular location oPL mRNA at five stages pregnancy (days 60, 90, 105, 120, 135) 21 cross-bred ewes, results were compared maternal serum concentrations, cotyledonary DNA actin total weight. The tissue increased (P < or = 0.05) from day 60 (15.4 pg/micrograms RNA) 120 (73.7 gestation then...
Ovine and bovine trophoblast protein-1 (oTP-1 bTP-1) are newly discovered proteins produced by embryonic tissues for a limited period in early gestation. They appear to act as agents that prevent regression of the corpus luteum during pregnancy ewe cow. TP-1 [mol wt (Mr), 17,000] consists three or four isoelectric variants (pI 5.4-5.7), whereas bTP-1, which cross-reacts with antiserum oTP-1, is found two predominant Mr classes (Mr, 22,000 24,000), each several (in pI range 6.3-6.8)....