A5054009040- RNA regulation and disease
- RNA modifications and cancer
- Atherosclerosis and Cardiovascular Diseases
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Adipokines, Inflammation, and Metabolic Diseases
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Circular RNAs in diseases
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- interferon and immune responses
- HIV/AIDS drug development and treatment
- Gut microbiota and health
- Alzheimer's disease research and treatments
- Pluripotent Stem Cells Research
- COVID-19 Clinical Research Studies
- Neuroscience and Neural Engineering
- Telomeres, Telomerase, and Senescence
- Neuroscience and Neuropharmacology Research
- CRISPR and Genetic Engineering
- Pneumocystis jirovecii pneumonia detection and treatment
Oslo University Hospital
2020-2024
University of Oslo
2020-2023
KU Leuven
2015-2018
Leuven Institute for Fertility and Embryology
2018
We recently showed that interleukin (IL)-6 inhibition by tocilizumab improves myocardial salvage in ST-elevation infarction (STEMI). However, the mechanisms for this effect are not clear.In exploratory sub-study of ASSAIL-MI trial, we examined leukocyte differential counts and their relation to peak troponin T (TnT) STEMI patients randomised (n = 101) or placebo 98). performed RNA-sequencing on whole blood 40) cells 20). B cell subpopulations were flow cytometry 69).(i) had higher neutrophil...
Abstract Introduction Tauopathies are neurodegenerative diseases characterized by TAU protein–related pathology, including frontotemporal dementia and Alzheimer's disease among others. Mutant animal models available, but none of them faithfully recapitulates human pathology not suitable for drug screening. Methods To create a new in vitro tauopathy model, we generated footprint‐free triple MAPT ‐mutant induced pluripotent stem cell line (N279K, P301L, E10+16 mutations) using clustered...
More than 170 post-transcriptional RNA modifications regulate the localization, processing and function of cellular RNAs, aberrant have been linked to a range human diseases. The modification landscape in atherosclerosis, main underlying cause cardiovascular diseases, is still largely unknown.We used mass spectrometry analyse selection RNA-modifying enzymes N6-methyladenosine (m6A) carotid atherosclerotic lesion samples representing early advanced stages atherosclerosis as compared...
Atherogenesis involves a complex interaction between immune cells and lipids, processes greatly influenced by the vascular smooth muscle cell (VSMC) phenotype. The DNA glycosylase NEIL3 has previously been shown to have role in atherogenesis, though whether this is due its ability repair damage or other non-canonical functions not yet clear. Hereby, we investigate of specifically VSMC phenotypic modulation, which critical plaque formation stability.Chow diet-fed atherosclerosis-prone Apoe-/-...
Abstract Background T‐cell activation is associated with an adverse outcome in COVID‐19, but whether and exhaustion relate to persistent respiratory dysfunction death unknown. Objectives To investigate persist are prolonged after hospitalization for COVID‐19. Methods Plasma serum from two Norwegian cohorts of hospitalized patients COVID‐19 ( n = 414) were analyzed soluble (s) markers (sCD25) (sTim‐3) during follow‐up. Results Both strongly acute failure, only sTim‐3 was independently 60‐day...
Background In cardiovascular diseases, atherosclerotic disorder are the most frequent and important with respect to morbidity mortality. Inflammation mediated by immune cells is central in all parts of progress, further understanding underlying mechanisms needed. Growing evidence suggests that deamination adenosine‐to‐inosine RNA crucial for a correct response; nevertheless, role editing atherogenesis has barely been studied. Several proteins have affinity inosines RNA, one being ENDOV...
Gene therapy holds promise to cure a wide range of genetic and acquired diseases. Recent successes in recombinant adeno-associated viral vector (rAAV)-based gene the clinic for hereditary disorders such as Leber's congenital amaurosis hemophilia B encouraged us reexplore an rAAV approach pulmonary transfer. Only limited clinical have been achieved airway transfer so far, underscoring need further preclinical development rAAV-based disorders. We sought determine potential airway-tropic...
Endonuclease V (EndoV) is a conserved inosine-specific ribonuclease with unknown biological function. Here, we present the first mouse model lacking EndoV, which viable without visible abnormalities. We show that endogenous murine EndoV cleaves inosine-containing RNA in vitro, nevertheless series of experiments fails to link an vivo function processing such transcripts. As inosine levels and adenosine-to-inosine editing often are dysregulated hepatocellular carcinoma (HCC), chemically...
Atherosclerosis and its consequences cause considerable morbidity mortality world-wide. We have previously shown that expression of the DNA glycosylase NEIL3 is regulated in human atherosclerotic plaques, NEIL3-deficiency enhances atherogenesis Apoe-/- mice. Herein, we identified a time point prior to quantifiable differences atherosclerosis between Apoe-/-Neil3-/- mice Mice at this age were selected explore metabolic pathophysiological processes preceding extensive NEIL3-deficient...
Deficiency of NEIL3, a DNA repair enzyme, has significant impact on mouse physiology, including vascular biology and gut health, processes related to aging. Leukocyte telomere length (LTL) is suggested as marker biological aging, shortened LTL associated with increased risk cardiovascular disease. NEIL3 been shown damage in regions vitro. Herein, we explored the role maintenance vivo by studying bone marrow cells from atherosclerosis-prone NEIL3-deficient mice. We found telomeres decreased...
Obesity is a complex multicausal disease that can cause morbidity and mortality, there need for improved knowledge on the underlying mechanisms. Using mouse model of increased T cell responsiveness, we show development obesity be driven by immune cells. This was confirmed with bone marrow transplantation adoptive transfer to several recipient models. Single-cell RNA sequencing CyTOF analysis showed mice display altered composition circulating cells activation in visceral adipose tissue,...
Circular (circ) RNAs are non-coding with important functions in the nervous system, cardiovascular and cancer. Their role atherosclerosis myocardial infarction (MI) remains poorly described. We aim to investigate potential circRNAs immune cells during atherogenesis examine most regulated MI modulation by interleukin (IL)-6 receptor inhibition tocilizumab. Wild-type (WT)
The past decades in Alzheimer's Disease (AD) research focused on the understanding of role amyloid beta and tau-protein aggregates pathophysiology AD. At least 30 mutations tau gene (MAPT) have been associated with familial forms frontotemporal dementia (FTD), shown to increase aggregation drive synaptic failure observed during disease progression. However, normal neuronal function still remains elusive has only studied set-ups low scalability limited translational value. As induced...