A5073127729- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Cancer Research and Treatments
- Bacteriophages and microbial interactions
- Nanoplatforms for cancer theranostics
- Antimicrobial Peptides and Activities
- Vibrio bacteria research studies
- HIV Research and Treatment
- Immune cells in cancer
- HIV-related health complications and treatments
- Antibiotics Pharmacokinetics and Efficacy
- Virus-based gene therapy research
- Bacterial Identification and Susceptibility Testing
- Tryptophan and brain disorders
- Polyamine Metabolism and Applications
- Phytochemicals and Medicinal Plants
- Environmental Chemistry and Analysis
- Antibiotic Use and Resistance
- Bacterial Genetics and Biotechnology
- Immune Response and Inflammation
- bioluminescence and chemiluminescence research
- Immune Cell Function and Interaction
- Nanoparticle-Based Drug Delivery
- Curcumin's Biomedical Applications
- Natural Antidiabetic Agents Studies
Karolinska Institutet
2023-2025
Olink Bioscience (Sweden)
2025
Sir H.N. Reliance Foundation Hospital and Research Centre
2024
World Health Organization Regional Office for South-East Asia
2022
Medizinische Hochschule Hannover
2014-2020
Helmholtz Centre for Infection Research
2014-2020
Center for Experimental and Clinical Infection Research
2014-2020
Savitribai Phule Pune University
2011
Institute of Bioinformatics
2011
A tropical marine bacterium isolated from the hard coral, Symphyllia sp. was identified as Serratia marcescens on basis of morphological, biochemical and 16S rDNA analysis. The showed antimicrobial activity towards pathogens Candida albicans Pseudomonas aeruginosa biofouling Bacillus pumilus. S. displayed biosurfactant evidenced by drop collapse, blood hemolysis surface tension reduction (52.0-27 mN m(-1)). active compound purified solvent extraction silicic acid chromatography....
Curcumin has been widely used owing to its various medicinal properties including antitumor effects. However, clinical application is limited by instability, poor solubility and low bioavailability. Folic acid (FA)-functionalized nanoformulations may enhance the sustained release of an anticancer drug (curcumin) tumor-specific targeting improve therapeutic benefit. This study aims design a nanoconjugate (NC) comprised folate-curcumin-loaded gold-polyvinylpyrrolidone nanoparticles...
ABSTRACT The recent finding that high numbers of strict anaerobes are present in the respiratory tract cystic fibrosis (CF) patients has drawn attention to pathogenic contribution CF microbiome airway disease. In this study, we investigated specific interactions most dominant bacterial pathogen, Pseudomonas aeruginosa , with anaerobic bacterium Veillonella parvula which been recovered at comparable cell from patients. addition growth competition experiments, transcriptional profiling, and...
Cells of the myeloid lineage, particularly monocytes and macrophages, play a key role in HIV infection by contributing to viral replication, immune response, maintaining balance during suppressive therapy. We hypothesized that metabolic reprogramming altered chemokine signaling people living with (PWH) on long-term antiretroviral therapy (ART) affect monocyte transport polarization due ongoing inflammation. Therefore, present study aimed identify mechanism impaired monocyte/macrophage...
Abstract Risk stratification using multi‐omics data deepens understanding of immunometabolism in successfully treated people with HIV (PWH) is inadequately explained. A personalized medicine approach integrating blood cell transcriptomics, plasma proteomics, and metabolomics employed to identify the mechanisms immunometabolic complications prolonged PWH from COCOMO cohort. Among PWHs, 44% are at risk experiencing identified network‐based patient method. Utilizing advanced machine learning...
To date, virulent bacteria remain the basis of most mediated cancer therapies. For clinical application attenuation is required. However, this might result in a drastically lowered therapeutic capacity. Herein we argue that E. coli probiotic Symbioflor-2, with history safe may constitute viable tumor candidate. We demonstrate Symbioflor-2 displays highly specific targeting ability as determined murine CT26 and RenCa models. The excellent specificity was ascribed to reduced levels adverse...
The potential of bacteria-mediated tumor therapy (BMTT) is highlighted by more than a century investigation. Attenuated Salmonella has prevailed as promising therapeutic agents. For BMTT - categorized an immune the exact contribution particular reactions to effect remains ambiguous. In addition, one could argue for or against requirement bacterial viability and targeting. Herein we evaluate isolated efficacy purified LPS TNF-α, which together account dominant immunogenic pathway gram...
Patients suffering from cystic fibrosis (CF) are commonly affected by chronic Pseudomonas aeruginosa biofilm infections. This is the main cause for high disease severity. In this study, we demonstrate that P. able to efficiently colonize murine solid tumors after intravenous injection and form biofilms in tissue. Biofilm formation was evident electron microscopy. Such structures could not be observed with transposon mutants, which were defective formation. Comparative transcriptional...
Biofilm-residing bacteria embedded in an extracellular matrix are protected from diverse physicochemical insults. In addition to the general recalcitrance of biofilm bacteria, high bacterial loads biofilm-associated infections significantly diminish efficacy antimicrobials due a low per-cell antibiotic concentration. Accordingly, present antimicrobial treatment protocols that have been established serve eradication acute fail clear chronic infections. study, we applied automated confocal...
Summary Pseudomonas aeruginosa is an environmental microorganism and a causative agent of diverse acute chronic, biofilm‐associated infections. Advancing research‐based knowledge on its adaptation to conditions within the human host bound reveal novel strategies targets for therapeutic intervention. Here, we investigated traits that P. PA14 as well virulence attenuated Δ lasR mutant need survive in selected murine infection models. Experimentally, genetic programs bacteria use adapt versus...
Embedded in an extracellular matrix, biofilm-residing bacteria are protected from diverse physicochemical insults. In accordance, the human host general recalcitrance of biofilm-grown hinders successful eradication chronic, biofilm-associated infections. this study, we demonstrate that upon addition promethazine, FDA approved drug, antibiotic tolerance vitro can be abolished. We show following antibiotics capable efficiently killing cells at minimal inhibitory concentrations. Synergistic...
Pseudomonas aeruginosa is an opportunistic pathogen that can thrive by anaerobic respiration in the lungs of cystic fibrosis patients using nitrate as terminal electron acceptor. Here, we report identification and characterization small RNA PaiI P. strain 14 (PA14). anaerobically induced presence depends on two-component system NarXL. Our studies revealed required for efficient denitrification affecting conversion nitrite to nitric oxide. In absence growth was impaired glucose, which be...
Summary Myeloid-derived suppressor cells (MDSC) are immature myeloid with immunomodulatory function. To study the mechanism by which MDSC affect antimicrobial immunity, we infected mice two M. avium strains of differential virulence, highly virulent Mycobacterium subsp. strain 25291 (MAA) and low hominissuis 104 (MAH). Intraperitoneal infection MAA, but not MAH, caused severe disease massive splenic infiltration monocytic (M-MDSC; Gr-1intCD11bhiCD11cint) expressing inducible NO synthase...
Genetically engineered Salmonella Typhimurium are potent vectors for prophylactic and therapeutic measures against pathogens as well cancer. This is based on the adjuvanticity that supports strong immune responses. The physiology of understood. It simplifies engineering both enhanced immune‐stimulatory properties safety features, thus, resulting in an appropriate balance between attenuation efficacy clinical applications. A major virulence factor flagellum. also a pathogen‐associated...
Exploring the complexity of host–pathogen communication is vital to understand why microbes persist within a host, while others are cleared. Here, we employed dual-sequencing approach unravel conversational turn-taking dynamic communications. We demonstrate that upon hitting host cell, motile Pseudomonas aeruginosa induce specific gene expression program. This results in spermidine on surface, which specifically activates PIP 3 -pathway phagocytic uptake into primary or immortalized murine...
INTRODUCTION: Antimicrobial resistance is rapidly invading the hospital settings worldwide, threatening ability to treat common infectious diseases. Stewardship has been adopted by many institutions enhance clinical outcomes, while diminishing unintended consequences of antibiotics including antimicrobial resistance.METHODOLOGY: In first phase Programme (ASP) conducted at a tertiary 345-bedded in South Mumbai, list "Reserve" (cautionary) antimicrobials was prepared and circulated, followed...
Personalized health profiling using multi-omics data can uncover immunometabolic disruptions in people living with HIV (PWH) who are successfully undergoing antiretroviral therapy (ART). Here we integrated transcriptomics, proteomics, and metabolomics to examine impairments treated PWH (n=158). Considering clinical lifestyle factors, identified 44%(70/158) of were at risk experiencing complications. Utilizing advanced machine learning techniques a Bayesian classifier, discovered six plasma...
Introduction: Yesaka Liquid is an Ayurvedic Proprietary formulation containing multiple standardized herbal extracts like Triphala (3 myrobalans), Jamun (Eugenia jambolana), Kutki (Picrorhiza kurrooa), Haridra (Curcuma longa) etc., having potential anti-diabetic and anti-oxidant properties. The evaluation of clinical efficacy safety as Add-on therapy to oral hypoglycemic agents (OHAs) in Type II diabetics was the primary objective study. Methods: After obtaining ethical approval informed...