A5057430740- Mycobacterium research and diagnosis
- Streptococcal Infections and Treatments
- Pharmacological Effects of Natural Compounds
- Neonatal and Maternal Infections
- Ginseng Biological Effects and Applications
- Antimicrobial Resistance in Staphylococcus
- Immune Cell Function and Interaction
- Infectious Diseases and Mycology
- Tuberculosis Research and Epidemiology
- Microbial infections and disease research
- Infective Endocarditis Diagnosis and Management
- Toxin Mechanisms and Immunotoxins
- Clostridium difficile and Clostridium perfringens research
- Inflammatory Bowel Disease
- Plant Pathogenic Bacteria Studies
- Infections and bacterial resistance
- Vector-borne infectious diseases
- Bartonella species infections research
- Bacteriophages and microbial interactions
- Helicobacter pylori-related gastroenterology studies
- Viral Infections and Vectors
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Cytokine Signaling Pathways and Interactions
- Gut microbiota and health
University of Veterinary Medicine Hannover, Foundation
2014-2023
Temple University Health System
2017
University of Veterinary Medicine Vienna
2012-2015
Medizinische Hochschule Hannover
2002-2011
Hochschule Hannover
2004-2008
Institut für Tier-, Natur- und Umweltethik
1998-2002
Institute of Pathology Celle
1997-2001
On March 24 and 25, 2017 researchers clinicians from around the world met at Temple University in Philadelphia to discuss current knowledge of Mycobacterium avium ssp. Paratuberculosis (MAP) its relationship human disease. The conference was held because shared concern that MAP is a zoonotic bacterium poses threat not only animal health but also health. In order further study this problem, conferees discussed ways improve diagnostic tests potential future anti-MAP clinical trials....
The phagosomes containing viable pathogenic mycobacteria, such as Mycobacterium (M.) tuberculosis and avium ssp. (M. avium), are known to be limited in their ability both acidify fuse with late (but not early) endocytic organelles. Here, we analysed the pH fusogenicity of M. paratuberculosis ptb), causative agent ruminants. Using murine J774 macrophage cell line, compared heat-killed ptb and, addition, or dead avium, well two non-pathogenic smegmatis gordonae. Electron microscopic analysis...
Streptococcus suis is a porcine and human pathogen causing invasive diseases, such as meningitis or septicaemia. Host cell interactions of S. have been studied mainly with serotype 2 strains, but multiple capsular serotypes well non-typeable strains exist diverse virulence features. At present, considered an extracellular pathogen. However, whether not it can also invade host cells matter controversial discussions. We assessed adherence invasion for HEp-2 epithelial by comparing 10 four (NT)...
Streptococcus suis is one of the most important pathogens in pigs and also an emerging zoonotic agent. After crossing epithelial barrier, S. causes bacteraemia, resulting meningitis, endocarditis bronchopneumonia. Since host environment seems to be regulatory component for virulence, we related expression virulence determinants glucose availability during growth sugar metabolism regulator c atabolite ontrol p rotein A (CcpA). We found that virulence-associated genes arcB , representing...
ABSTRACT Streptococcus suis is an important cause of infectious diseases in young pigs. Little known about the virulence factors or protective antigens S. . Recently, we have identified two proteins arginine deiminase system (ADS) , which were temperature induced and expressed on streptococcal surface (N. Winterhoff, R. Goethe, P. Gruening, M. Rohde, H. Kalisz, E. Smith, Valentin-Weigand, J. Bacteriol. 184:6768-6776, 2002). In present study, analyzed complete ADS Due to their homologies...
ABSTRACT Streptococcus suis serotype 2 is a porcine and human pathogen with adhesive invasive properties. In other streptococci, large surface-associated proteins (>100 kDa) of the MSCRAMM family (microbial surface components recognizing matrix molecules) are key players in interactions host tissue. this study, we identified novel opacity factor S. (OFS) structural homology to members family. The N-terminal region OFS homologous respective regions fibronectin-binding protein A (FnBA)...
Streptococcus suis causes numerous diseases in pigs, most importantly, meningitis, arthritis, septicemia, and bronchopneumonia. One of the major problems modern swine production is lack a vaccine protecting against more than one S. serotype. The objective this study was to determine protective efficacy serotype 2 murein-associated protein (MAP) fraction subunit comparison that bacterin experimental challenge with (containing muramidase-released [MRP], extracellular factor, suilysin [SLY]) 9...
Streptococcus suis is one of the most important pathogens in pigs and can also cause severe infections humans. Despite its clinical relevance, very little known about factors that contribute to virulence. Recently, we identified a new putative virulence factor S. , arginine deiminase system (ADS), an catabolic enzyme encoded by arcABC operon, which enables survive acidic environment. In this study, focused on ArgR, ADS-associated regulator belonging ArgR/AhrC repressor family. Using argR...
Streptococcus suis (S. suis) is a neglected zoonotic streptococcus causing fatal diseases in humans and pigs. The transcriptional regulator CcpA (catabolite control protein A) involved the metabolic adaptation to different carbohydrate sources virulence of S. other pathogenic streptococci. In this study, we determined DNA binding characteristics identified regulon during growth suis. Electrophoretic mobility shift analyses showed promiscuous cognate cre sites vitro. contrast, sequencing...
Persister cells constitute a subpopulation of dormant within microbial population which are genetically identical but phenotypically different to regular cells. Notably, persister show an elevated tolerance antimicrobial agents. Thus, they considered represent 'bet-hedging' strategy and particular importance in pathogenic bacteria. We studied the ability zoonotic pathogen Streptococcus (S.) suis form multi-drug tolerant variants identified dependent on initial bacterial growth phase....
Type I interferons (IFN-I), such as IFN-α and IFN-β are important messengers in the host response against bacterial infections. Knowledge about role of IFN-I infections by nontuberculous mycobacteria (NTM) is limited. Here we show that macrophages infected with pathogens Mycobacterium avium complex produced significantly lower amounts than opportunistic pathogen M. smegmatis. To dissect molecular mechanisms this phenomenon, focused on obligate ssp paratuberculosis (MAP) Viability both...
The probiotic bacterial strain Enterococcus faecium SF68 has been shown to alleviate symptoms of intestinal inflammation in human clinical trials and animal feed supplementation studies. To identify factors involved immunomodulatory effects on host cells, E. other commensal isolates were screened using epithelial cell lines harboring reporter fusions for NF-κB JNK(AP-1) activation determine the responses innate immune signaling pathways when challenged with protein components. Cell-free,...
ABSTRACT The present study was performed to identify stress-induced putative virulence proteins of Streptococcus suis . For this, protein expression patterns streptococci grown at 32, 37, and 42°C were compared by one- two-dimensional gel electrophoresis. Temperature shifts from 32 37 induced two cell wall-associated with apparent molecular masses approximately 47 53 kDa. Amino-terminal sequence analysis the indicated homologies 47-kDa an ornithine carbamoyltransferase (OCT) pyogenes 53-kDa...
ABSTRACT We evaluated the genetic diversity of Streptococcus suis isolates different serotypes by macrorestriction analysis and elucidated possible relationships between background, expression potential virulence traits, source isolation. Virulence traits included serotype-specific polysaccharides, muramidase-released protein (MRP), extracellular factor (EF), hemolysin activity, adherence to epithelial cells. Macrorestriction streptococcal DNA digested with restriction enzymes Sma I Apa...
Abstract Mycobacterium avium subspecies paratuberculosis (MAP) causes a chronic enteritis in ruminants. In addition, MAP is presently the most favored pathogen linked to Crohn’s disease. this study, we were interested dissecting molecular mechanisms of macrophage activation or deactivation after infection with MAP. By subtractive hybridization cDNAs, identified immune-responsive gene 1 (IRG1), which was expressed substantially higher lipopolysaccharide (LPS)-stimulated than MAP-infected...
The arginine-ornithine antiporter (ArcD) is part of the Arginine Deiminase System (ADS), a catabolic, energy-providing pathway found in variety different bacterial species, including porcine zoonotic pathogen Streptococcus suis. ADS has recently been shown to play role pathogenicity S. suis, particular its survival host cells. contribution arginine and transport mediated by ArcD, however, yet be clarified. In present study, we showed experiments using [U-13C6]arginine as tracer molecule that...
La Crosse virus (LACV) is a mosquito-transmitted arbovirus and the main cause of virus-mediated neurological diseases in children. To date, little known about role C-type lectin receptors (CLRs)—an important class pattern recognition receptors—in LACV recognition. DC-SIGN remains only well-described CLR that recognizes LACV. In this study, we investigated additional CLR/LACV interactions. end, applied flow-through chromatography method for purification to perform an unbiased high-throughput...