A5044800995- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- Bacterial biofilms and quorum sensing
- Metal Extraction and Bioleaching
- Microbial Fuel Cells and Bioremediation
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Mine drainage and remediation techniques
- Marine and coastal ecosystems
- Gene Regulatory Network Analysis
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Arsenic contamination and mitigation
- Microbial Metabolic Engineering and Bioproduction
- Plant Disease Management Techniques
- Aquatic Ecosystems and Biodiversity
- Methane Hydrates and Related Phenomena
- CRISPR and Genetic Engineering
- Environmental and Cultural Studies in Latin America and Beyond
- Aquatic Ecosystems and Phytoplankton Dynamics
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Mineral Processing and Grinding
- Nematode management and characterization studies
Universidad de Sevilla
2025
Technische Universität Braunschweig
2014-2024
GenXPro (Germany)
2024
Helmholtz Centre for Infection Research
2016-2023
Centro Nacional de Biotecnología
2015-2020
Consejo Superior de Investigaciones Científicas
2010-2012
The ‘Standard European Vector Architecture’ database (SEVA-DB, http://seva.cnb.csic.es) was conceived as a user-friendly, web-based resource and material clone repository to assist in the choice of optimal plasmid vectors for de-constructing re-constructing complex prokaryotic phenotypes. SEVA-DB adopts simple design concepts that facilitate swapping functional modules extension genome engineering options microorganisms beyond typical laboratory strains. Under SEVA standard, every DNA...
Cyanobacteria are ecologically versatile microorganisms inhabiting most environments, ranging from marine systems to arid deserts. Although they possess several pathways for light-independent energy generation, until now their ecological range appeared be restricted environments with at least occasional exposure sunlight. Here we present molecular, microscopic, and metagenomic evidence that cyanobacteria predominate in deep subsurface rock samples the Iberian Pyrite Belt Mars analog...
Pseudomonas aeruginosa (PA) is an opportunistic human pathogen, which involved in a wide range of dangerous infections. It develops alarming resistances toward antibiotic treatment. Therefore, alternative strategies, suppress pathogenicity or synergize with treatments are great need to combat these infections more effectively. One promising approach disarm the bacteria by interfering their quorum sensing (QS) system, regulates release various virulence factors as well biofilm formation....
The overall success of a pathogenic microbe depends on its ability to efficiently adapt challenging conditions in the human host. Long-term evolution experiments track and predict adaptive trajectories have contributed significantly our understanding driving forces bacterial adaptation. In this study, we conducted cross-sectional study instead long-term longitudinal experiments. We analyzed transcriptional profiles as well genomic sequence variations large number clinical Pseudomonas...
Microbial activity is a major contributor to the biogeochemical cycles that make up life support system of planet Earth. A 613 m deep geomicrobiological perforation and systematic multi-analytical characterization revealed an unexpected diversity associated with rock matrix microbiome operates in subsurface Iberian Pyrite Belt (IPB). Members 1 class 16 genera were deemed most representative microorganisms IPB selected for deeper analysis. The use fluorescence situ hybridization allowed not...
The soil bacterium Pseudomonas putida injects toxic proteins into neighbouring competitors including resilient phytopathogens using the Type VI secretion system (T6SS). of toxins endows P. a significant fitness advantage, allowing this biocontrol agent to thrive in plant-related polymicrobial environments and prevent phytopathogen infections. However, despite significance agriculture biotechnology, identity mechanisms action most its remain largely unexplored. This is part because only one...
In light of the antibiotic crisis, emerging strategies to sensitize bacteria available antibiotics should be explored. Several studies on mechanisms killing suggest that bactericidal activity is enforced through generation reactive oxygen species (ROS-lethality hypothesis). Here, we artificially manipulated redox homeostasis model opportunistic pathogen Pseudomonas aeruginosa using specific enzymes catalyze either formation or oxidation NADH. Increased NADH levels led activation efflux pumps...
Summary The genome of the soil bacterium Pseudomonas putida KT2440 encodes singular orthologues genes crp (encoding catabolite repression protein, Crp) and cyaA (adenylate cyclase) Escherichia coli. levels cAMP formed by P. cells were below detection with a Dictyostelium biosensor in vivo. P.putida gene was transcribed vivo but failed to complement lack maltose consumption mutant E. coli , thereby indicating that poorly translated or rendered non‐functional heterologous host. Yet, generation...
The ability of Pseudomonas species to thrive in all major natural environments (i.e. terrestrial, freshwater and marine) is based on its exceptional capability adapt physicochemical changes. Thus, environmental bacteria have tightly control the maintenance numerous physiological traits across different conditions. intracellular pH (pHi ) homoeostasis a particularly important feature, since pHi influences large portion biochemical processes cell. Despite importance, relatively few reliable,...
The natural physiological regime of the soil bacterium Pseudomonas putida involves incessant exposure to endogenous metabolic conflicts and environmental physicochemical insults. Yet, role assisted small RNA-mRNA pairing in stress tolerance super-phenotype that is trademark this has not been accredited. We have thoroughly explored consequences -in particular those related exogenous - deleting hfq gene P. putida, which encodes major RNA chaperone promotes sRNA-target mRNA interactions. While...
Embedded in an extracellular matrix, biofilm-residing bacteria are protected from diverse physicochemical insults. In accordance, the human host general recalcitrance of biofilm-grown hinders successful eradication chronic, biofilm-associated infections. this study, we demonstrate that upon addition promethazine, FDA approved drug, antibiotic tolerance vitro can be abolished. We show following antibiotics capable efficiently killing cells at minimal inhibitory concentrations. Synergistic...
Although the genome of Pseudomonas putida KT2440 encodes an orthologue crp gene Escherichia coli (encoding cAMP receptor protein), regulatory scope this factor seems to be predominantly co-opted in bacterium for controlling non-metabolic functions. In order investigate reasons such a functional divergence otherwise nearly identical proteins, Crp regulator P. (Crp(P. putida)) was purified apparent homogeneity and subject battery vitro assays aimed at determining its principal physicochemical...
Bacterial membrane vesicles (BMVs) are produced by most bacteria and participate in various cellular processes, such as intercellular communication, nutrient exchange, pathogenesis. Notably, these can contain virulence factors, including toxic proteins, DNA, RNA. Such factors contribute to the harmful effects of bacterial pathogens on host cells tissues. Although general BMVs physiology well known, underlying molecular mechanisms less understood. In this study, we introduce a vesicle...
Summary Although the soil bacterium Pseudomonas putida KT2440 bears a bona fide adenylate cyclase gene ( cya A), intracellular concentrations of 3′,5′‐cyclic adenosine monophosphate (cAMP) are barely detectable. By using reporter technology and direct quantification cAMP under various conditions, we show that such low levels molecule stem from stringent regulation its synthesis, efflux degradation. Poor production was result inefficient translation cyaA mRNA. Moreover, deletion...
Mesenchymal stromal cells (MSCs) hold promise for cell-based therapies due to their ability stimulate tissue repair and modulate immune responses. Umbilical cord-derived MSCs from Wharton jelly (WJ) offer advantages such as low immunogenicity potent modulatory effects. However, ensuring consistent quality safety throughout manufacturing process remains critical. RNA sequencing (RNA-seq) emerges a crucial tool assessing genetic stability expression dynamics in therapeutic products.
Abstract Although inducible heterologous expression systems have been available since the birth of recombinant DNA technology, diversity genetic devices and their coming together in corresponding vectors often result a lack reproducibility interoperability. In an effort to increase predictability genes interest variety possible bacterial hosts, we propose composition standard for debugging reassembling all regulatory parts that participate performance such devices. As case study, addressed...
Abstract Based on the analysis of 16S rRNA gene metabarcoding, here we report shift in microbial community structure along a horizontal oxygen gradient (0.40-6.06 mg L −1 ) volcanic influenced acid rock drainage (VARD) environment, known as San Cayetano (Cartago, Costa Rica; pH =2.94-3.06, sulfate ~0.87-1.19 g , iron ~35-61 ). This VARD is dominated by microorganisms involved geochemical cycling iron, sulfur and nitrogen; however, identity species changes with river course. The anoxic spring...
Abstract In this work, we characterize the geochemistry and microbial community of Bajo las Peñas, a neutral (pH 6.5-7.4), hot spring (T = 62.0-68.0°C) located near Turrialba Volcano, Costa Rica. The microbiota at its two sources belongs mainly to family Aquificae, comprising OTUs closely related genera Sulfurihydrogenibium , Thermosulfidibacter Thermodesulfovibrio Thermocrinis which is consistent with presence moderate levels sulfate (243-284 mg/L) along stream. We determined dramatic shift...