A5080081384- Social Sciences and Governance
- Bacterial Genetics and Biotechnology
- Social Policies and Family
- Bacteriophages and microbial interactions
- Healthcare Systems and Practices
- CRISPR and Genetic Engineering
- Education, sociology, and vocational training
- RNA and protein synthesis mechanisms
- Legal and Labor Studies
- Labor Movements and Unions
- Microbial Metabolic Engineering and Bioproduction
- Bacterial biofilms and quorum sensing
- French Urban and Social Studies
- Genomics and Phylogenetic Studies
- Gene Regulatory Network Analysis
- Employment, Labor, and Gender Studies
- Employment and Welfare Studies
- Aging, Elder Care, and Social Issues
- Health, Medicine and Society
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Evolution and Genetic Dynamics
- Occupational Health and Safety in Workplaces
- International Labor and Employment Law
- Agriculture and Rural Development Research
Centro Nacional de Biotecnología
2015-2025
Universidad Autónoma de Madrid
2019-2023
Linköping University
2018-2022
Okayama University
2018-2022
Universidad de Murcia
2018-2022
Hudson Institute
2018-2022
North Carolina State University
2022
North Central State College
2022
Universidade Federal de Minas Gerais
2022
University of the Western Cape
2022
The ‘Standard European Vector Architecture’ database (SEVA-DB, http://seva.cnb.csic.es) was conceived as a user-friendly, web-based resource and material clone repository to assist in the choice of optimal plasmid vectors for de-constructing re-constructing complex prokaryotic phenotypes. SEVA-DB adopts simple design concepts that facilitate swapping functional modules extension genome engineering options microorganisms beyond typical laboratory strains. Under SEVA standard, every DNA...
The genome of the soil bacterium Pseudomonas putida strain KT2440 has been erased various determinants resistance to antibiotics encoded in its extant chromosome. To this end, we employed a coherent genetic platform that allowed precise deletion multiple genomic segments large variety Gram-negative bacteria including (but not limited to) P. putida. method is based on obligatory recombination between free-ended homologous DNA sequences are released as linear fragments generated upon cleavage...
Bacteria form dense surface-associated communities known as biofilms that are central to their persistence and how they affect us. Biofilm formation is commonly viewed a cooperative enterprise, where strains species work together for common goal. Here we explore an alternative model: biofilm response ecological competition. We co-cultured diverse collection of natural isolates the opportunistic pathogen Pseudomonas aeruginosa studied effect on formation. show strain mixing reliably increases...
Because of its adaptability to sites polluted with toxic chemicals, the model soil bacterium Pseudomonas putida is naturally endowed a number metabolic and stress-endurance qualities which have considerable value for hosting energy-demanding redox reactions thereof. The growing body knowledge on P. strain KT2440 has been exploited rational design derivative in genome heavily edited order construct robust microbial cell factory.Eleven non-adjacent genomic deletions, span 300 genes (i.e., 4.3%...
The Standard European Vector Architecture 2.0 database (SEVA-DB 2.0, http://seva.cnb.csic.es) is an improved and expanded version of the platform released in 2013 (doi: 10.1093/nar/gks1119) aimed at assisting choice optimal genetic tools for de-constructing re-constructing complex prokaryotic phenotypes. By adopting simple compositional rules, SEVA standard facilitates combinations functional DNA segments that ease both analysis engineering diverse Gram-negative bacteria fundamental or...
Abstract Background Since publication in 1977 of plasmid pBR322, many breakthroughs Biology have depended on increasingly sophisticated vector platforms for analysis and engineering given bacterial strains. Although restriction sites impose a certain format the procedures assembling cloned genes, every attempt thus far to standardize architecture nomenclature has ended up failure. While this state affairs may still be tolerable traditional one-at-a-time studies single onset systems synthetic...
Although the flagellar machinery of environmental bacteria endows cells with a phenomenal survival device, it also consumes much metabolic currency necessary for fuelling such vigorous nano-motor. The physiological cost flagella-related functions soil bacterium Pseudomonas putida KT2440 was examined and quantified through deletion ≈ 70 kb DNA segment genome (≈ 1.1%), which includes relevant structural regulatory genes in this micro-organism. resulting strain lacked protruding polar cords...
The Standard European Vector Architecture 3.0 database (SEVA-DB 3.0, http://seva.cnb.csic.es) is the update of platform launched in 2013 both as a web-based resource and material repository formatted genetic tools (mostly plasmids) for analysis, construction deployment complex bacterial phenotypes. period between first version SEVA-DB present time has witnessed several technical, computational conceptual advances genetic/genomic engineering prokaryotes that have enabled upgrading utilities...
The SEVA platform (https://seva-plasmids.com) was launched one decade ago, both as a database (DB) and physical repository of plasmid vectors for genetic analysis engineering Gram-negative bacteria with structure nomenclature that follows strict, fixed architecture functional DNA segments. While the current update keeps basic features earlier versions, has been upgraded not only many more ready-to-use plasmids but also expand range target species, harmonize assembly methods enable new...
Abstract Background Accumulation of inorganic polyphosphate (polyP), a persistent trait throughout the whole Tree Life, is claimed to play fundamental role in enduring environmental insults large variety microorganisms. The share polyP tolerance soil bacterium Pseudomonas putida KT2440 suite physicochemical stresses has been studied on background its capacity as host oxidative biotransformations. Results Cells lacking kinase (Ppk), which expectedly presented low intracellular level, were...
2.6% of the genome soil bacterium Pseudomonas putida KT2440 encodes phage-related functions, but burden such opportunistic DNA on host physiology is unknown. Each four apparently complete prophages borne by this strain was tested for stability, spontaneous excision and ability to cause lysis under various stressing conditions. While P3 (PP2266-PP2297) P4 (PP1532-1584) were discharged from at a detectable rate, their induction failed otherwise yield infective viruses. Isogenic P. derivatives...
Some strains of the soil bacterium Pseudomonas putida have become in recent years platforms choice for hosting biotransformations industrial interest. Despite availability many genetic tools this microorganism, genomic editing cell factory P. EM42 (a derivative reference strain KT2440) is still a time-consuming endeavor. In work we investigated vivo activity Ssr protein encoded by open reading frame T1E_1405 from DOT-T1E, plausible functional homologue β Red recombination system λ phage...
A key feature of biological systems is the emergence higher-order structures from interacting units, such as development tissues individual cells and elaborate divisions labor in insect societies. Little known, however, how evolutionary competition among individuals affects organization. Here we explore this link bacterial biofilms, concrete that are well known for structures. We present a mechanistic model cell growth at surface, show tension between nutrients can explain empirically...