A5006575930- HIV Research and Treatment
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- HIV-related health complications and treatments
- Immune Response and Inflammation
- HIV/AIDS Research and Interventions
- Inflammasome and immune disorders
- Peroxisome Proliferator-Activated Receptors
- interferon and immune responses
- Immune cells in cancer
- RNA regulation and disease
Karolinska Institutet
2021-2024
University Hospital Bonn
2021
Abstract Purpose of Review The main obstacle to an HIV-1 cure is the reservoir infected cells. While antiretroviral therapy (ART) eliminates virus effectively, it does not target reservoir. To eliminate cells, we need improved understanding maintenance and reactivation mechanisms, including influence chromatin. Recent Findings last years’ technological advances enable in-depth study reservoir, uncovering subsets proviral integration sites, single-cell nucleosome histone modifications. These...
HIV-1 infection induces a chronic inflammatory environment not restored by suppressive antiretroviral therapy (ART). As of today, the effect viral suppression and immune reconstitution in people living with (PLWH) has been well described but completely understood. Herein, we show how PLWH who naturally control virus (PLWHEC) have reduced proportion CD4+CCR6+ CD8+CCR6+ cells compared to on ART (PLWHART) negative controls (HC). Expression CCR2 was both CD4+, CD8+ classical monocytes PLWHEC...
The reservoir of latently HIV-1 infected cells is heterogeneous. To achieve an cure, the activatable proviruses must be eliminated while permanently silenced may tolerated. We have developed a method to assess proviral nuclear microenvironment in single cells. In cells, zinc finger protein tethered promoter produced fluorescent signal as interest came its proximity, such viral transactivator Tat when recruited nascent RNA. essential for replication. these we assessed activation and chromatin...
Intercellular transmission of the second messenger 2′,3′-cGAMP, synthesized by viral DNA sensor cGAMP synthase (cGAS), is a potent mode bystander activation during host defense. However, whether this mechanism also contributes to cGAS-dependent autoimmunity remains unknown. Here, using murine bone marrow transplantation strategy, we demonstrate that, in Trex1−/−-associated autoimmunity, shuttling from radioresistant immune cells induces NF-κB activation, interferon regulatory factor 3 (IRF3)...
Abstract HIV-1 infection establishes a reservoir of long-lived cells with integrated proviral DNA that can persist despite antiretroviral therapy (ART). Some these at anytime be reactivated and reinitiate infection. The mechanisms governing latency the transcriptional regulation provirus are complex have not yet been sufficiently described. Here, we identified role for histone H3 citrullination, post-translational modification catalyzed by protein-arginine deiminase type-4 (PADI4), in...
Abstract The reservoir of latently HIV-1 infected cells is heterogeneous. To achieve an cure, the activatable proviruses should be eliminated while permanently silenced may tolerated. We have developed a method to assess proviral nuclear microenvironment in single cells. In cells, zinc finger protein tethered promoter produced fluorescent signal as interest came its proximity, such viral transactivator Tat when recruited nascent RNA. essential for replication. these we assessed activation...
Abstract HIV-1 infection induces a chronic inflammatory environment not restored by suppressive antiretroviral therapy (ART). As of today, the effect viral suppression and immune reconstitution in people living with (PLWH) on long-term ART (PLWH ) is well-described. Herein, we show how PLWH who naturally control virus EC have reduced proportion CD4 + CCR6 cells compared to negative controls. Specifically, /CCR6 - exhibited uniqe proteomic profile modulated energy metabolism independent...