A5018474393- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Kruppel-like factors research
- Eosinophilic Disorders and Syndromes
- RNA Interference and Gene Delivery
- Retinoids in leukemia and cellular processes
- Chronic Lymphocytic Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Adenosine and Purinergic Signaling
- MicroRNA in disease regulation
- Acute Lymphoblastic Leukemia research
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Multiple Myeloma Research and Treatments
- Genomics and Rare Diseases
- Cytokine Signaling Pathways and Interactions
- Mesenchymal stem cell research
- Circular RNAs in diseases
- Corneal Surgery and Treatments
- T-cell and Retrovirus Studies
- Inflammatory Biomarkers in Disease Prognosis
- Immune cells in cancer
- Chemokine receptors and signaling
University of Modena and Reggio Emilia
2016-2025
Ferrari (Italy)
2014-2023
MRC Centre for Regenerative Medicine
2021
University of Padua
2006
Istituto di Chimica Biomolecolare
1996
Azienda Ospedaliero-Universitaria di Modena
1992-1993
Transformation to secondary myelofibrosis (MF) occurs as part of the natural history polycythemia vera (PPV‐MF) and essential thrombocythemia (PET‐MF). Although primary (PMF) MF are considered similar diseases managed similarly, there few studies specifically focused on latter. The aim this study was characterize mutation landscape, describe main clinical correlates prognostic implications mutations, in a series 359 patients with PPV‐MF PET‐MF. Compared PV ET, JAK2 V617F CALR mutated allele...
Abstract This study was aimed at the characterization of a gene expression signature pluripotent hematopoietic CD34+ stem cell in idiopathic myelofibrosis (IM), which would eventually provide novel pathogenetic insights and/or diagnostic/prognostic information. Aberrantly regulated genes were revealed by transcriptome comparative microarray analysis normal and IM cells; selected also assayed granulocytes. One-hundred seventy four differentially expressed identified part validated...
Extracellular nucleotides are potent signaling molecules mediating cell-specific biological functions, mostly within the processes of tissue damage and repair flogosis. We previously demonstrated that adenosine 5'-triphosphate (ATP) inhibits proliferation human bone marrow-derived mesenchymal stem cells (BM-hMSCs), while stimulating, in vitro vivo, their migration. Here, we investigated effects ATP on BM-hMSC differentiation capacity. Molecular analysis showed treatment modulated expression...
Mutations in the gene calreticulin (CALR) occur majority of JAK2- and MPL-unmutated patients with essential thrombocythemia (ET) primary myelofibrosis (PMF); identifying CALR mutations contributes to diagnostic pathway ET PMF. are heterogeneous spanning over exon 9, but all result a novel common protein C terminus. We developed polyclonal antibody against 17-amino-acid peptide derived from mutated that was used for immunostaining bone marrow biopsies. show this specifically recognized...
With the intent of dissecting molecular complexity Philadelphia-negative myeloproliferative neoplasms (MPN), we designed a target enrichment panel to explore, using next-generation sequencing (NGS), mutational status an extensive list 2000 cancer-associated genes and microRNAs. The genomic DNA granulocytes in vitro-expanded CD3+T-lymphocytes, as germline control, was target-enriched sequenced learning cohort 20 MPN patients Roche 454 technology. We identified 141 genuine somatic mutations,...
Abstract In type 1 diabetes, the appearance of islet autoantibodies indicates onset autoimmunity, often many years before clinical symptoms arise. While T cells play a major role in destruction pancreatic beta cells, molecular underpinnings promoting aberrant cell activation remain poorly understood. Here, we show that during autoimmunity an miR142-3p/Tet2/Foxp3 axis interferes with efficient induction regulatory (Treg) resulting impaired Treg stability mouse and human. Specifically,...
Abstract Monocyte Distribution Width (MDW), a new cytometric parameter correlating with cytomorphologic changes occurring upon massive monocyte activation, has recently emerged as promising early biomarker of sepsis. Similar to sepsis, monocyte/macrophage subsets are considered key mediators the life-threatening hyper-inflammatory disorder characterizing severe COVID-19. In this study, we longitudinally analyzed MDW values in cohort 87 COVID-19 patients consecutively admitted our hospital,...
The transcription factor MYB has a key role in hematopoietic progenitor cells (HPCs) lineage choice, by enhancing erythropoiesis at the expense of megakaryopoiesis. We previously demonstrated that controls erythroid versus megakaryocyte decision transactivating KLF1 and LMO2 expression. To further unravel molecular mechanisms through which affects fate decision, we performed integrative analysis miRNA mRNA changes MYB-silenced human primary CD34+ HPCs. Among miRNAs with highest number...
The development of Imatinib mesylate (IM), which targets the oncogenic BCR-ABL fusion protein, has greatly improved outcome Chronic Myeloid Leukemia (CML) patients. However, BCR-ABL-positive progenitors can be detected in CML patients complete cytogenetic response. Several evidence suggests that stem cells are intrinsically resistant to Tyrosine Kinase Inhibitors (TKI), and therefore they represent most likely candidate responsible for disease relapse.In this work, we investigated microRNA...
Abstract Somatic mutations of calreticulin (CALR) have been described in approximately 60–80% JAK2 and MPL unmutated Essential Thrombocythemia Primary Myelofibrosis patients. CALR is an endoplasmic reticulum (ER) chaperone responsible for proper protein folding calcium retention. Recent data demonstrated that the TPO receptor (MPL) essential development mutant-driven Myeloproliferative Neoplasms (MPNs). However, precise mechanism action mutants haven’t fully unraveled. In this study, we...
The rapid evolution of Next Generation Sequencing in clinical settings, and the resulting challenge variant reinterpretation given constantly updated information, require robust data management systems organized approaches. In this paper, we present iVar: a freely available highly customizable tool with user-friendly web interface. It represents platform for unified variants identified by different sequencing technologies. iVar accepts call format (VCF) files text annotation elaborates them,...
Abstract Clonal myeloproliferation and development of bone marrow (BM) fibrosis are the major pathogenetic events in myelofibrosis (MF). The identification novel antifibrotic strategies is utmost importance since effectiveness current therapies reverting BM debated. We previously demonstrated that osteopontin (OPN) has a profibrotic role MF by promoting mesenchymal stromal cells proliferation collagen production. Moreover, increased plasma OPN correlated with higher grade inferior overall...