A5014371052- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Chronic Lymphocytic Leukemia Research
- Melanoma and MAPK Pathways
- Phagocytosis and Immune Regulation
- Glioma Diagnosis and Treatment
- vaccines and immunoinformatics approaches
- Click Chemistry and Applications
- Immune Cell Function and Interaction
- Immune cells in cancer
- Lymphoma Diagnosis and Treatment
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- PI3K/AKT/mTOR signaling in cancer
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Kruppel-like factors research
- Cell death mechanisms and regulation
- Brain Metastases and Treatment
- Occupational and environmental lung diseases
- Single-cell and spatial transcriptomics
- Calcium signaling and nucleotide metabolism
- Advanced Breast Cancer Therapies
- Neuroinflammation and Neurodegeneration Mechanisms
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2015-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2025
University of Modena and Reggio Emilia
2011-2017
Abstract No prospective data were available prior to 2021 inform selection between combination BRAF and MEK inhibition versus dual blockade of programmed cell death protein-1 (PD-1) cytotoxic T lymphocyte antigen-4 (CTLA-4) as first-line treatment options for BRAFV 600-mutant melanoma. SECOMBIT (NCT02631447) was a randomized, three-arm, noncomparative phase II trial in which patients randomized one two sequences with immunotherapy or targeted therapy first, third arm an 8-week induction...
Macrophages reside in tissues infiltrated by chronic lymphocytic leukemia B cells and the extent of infiltration is associated with adverse prognostic factors. We studied blood monocyte population flow cytometry whole-genome microarrays. A mixed lymphocyte reaction was performed to evaluate proliferation T contact monocytes from patients normal donors. Migration gene modulation cultured CLL were also evaluated. The absolute number increased compared controls (792±86 cells/μL versus 485±46...
The transcription factor MYB has a key role in hematopoietic progenitor cells (HPCs) lineage choice, by enhancing erythropoiesis at the expense of megakaryopoiesis. We previously demonstrated that controls erythroid versus megakaryocyte decision transactivating KLF1 and LMO2 expression. To further unravel molecular mechanisms through which affects fate decision, we performed integrative analysis miRNA mRNA changes MYB-silenced human primary CD34+ HPCs. Among miRNAs with highest number...
Background Glioblastoma (GBM) is a poor prognosis grade 4 glioma. After surgical resection, the standard therapy consists of concurrent radiotherapy (RT) and temozolomide (TMZ) followed by TMZ alone. Our previous data on melanoma patients showed that Dendritic Cell vaccination (DCvax) could increase amount intratumoral-activated cytotoxic T lymphocytes Methods This single-arm, monocentric, phase II trial in two steps according to Simon’s design. The aims evaluate progression-free survival...
Lenalidomide is an immunomodulatory agent clinically active in chronic lymphocytic leukemia patients. The specific mechanism of action still undefined, but includes modulation the microenvironment. In patients, nurse-like cells differentiate from CD14+ mononuclear and protect apoptosis. Nurse-like resemble M2 macrophages with potent immunosuppressive functions. Here, we examined effect lenalidomide on monocyte/macrophage population We found that induces high actin polymerization monocytes...
Abstract Background The current therapeutic algorithm for Advanced Stage Melanoma comprises of alternating lines Targeted and Immuno-therapy, mostly via Immune-Checkpoint blockade. While Comprehensive Genomic Profiling solid tumours has been approved as a companion diagnostic, still no predictive biomarkers are available aside from BRAF mutations the controversial Tumor Mutational Burden. This study presents results Multi-Centre Observational Clinical Trial on Target Immuno-therapy treated...
The impact of the order treatment with checkpoint inhibitors or BRAF/MEK on development brain metastases in patients metastatic unresectable
The endothelin axis, comprising endothelins (ET-1, ET-2 and ET-3) their receptors (ETAR ETBR), has emerged as relevant player in tumor growth metastasis. Here, we investigated the involvement of ET-1/ETAR axis chronic lymphocytic leukemia (CLL). CLL cells expressed higher levels ET-1 ETA receptor compared to normal B cells. peptide stimulated phosphoinositide-3-kinase mitogen-activated protein kinase signaling pathways, improved survival promoted proliferation leukemic throughout ETAR...
Increasing evidence suggests a key role for angiopoietin-2 (ANGPT2) in influencing the aggressiveness of chronic lymphocytic leukemia (CLL). In presence vascular endothelial growth factor (VEGF), ANGPT2 causes vessel destabilization leading to neoangiogenesis. Accordingly, high expression levels and degree angiogenesis have consistently been associated with poor prognosis CLL; however, molecular mechanisms behind variability are still be discovered. Here, first time, we investigated DNA...
Dendritic cell (DC)-based vaccination effectively induces anti-tumor immunity although in the majority of cases this does not translate into a durable clinical response. However, DC is characterized by robust safety profile, making treatment potential candidate for effective combination cancer immunotherapy. To explore possibility, understanding changes occurring tumor microenvironment (TME) upon required. In line, quantitative and qualitative tumor-infiltrating T lymphocytes (TILs) induced...
Abstract Background Dendritic cells (DCs) are the most efficient antigen-presenting and play a central role in immune system, orchestrating response against tumors. We previously demonstrated that DC-based vaccination effectively induces anti-tumor immunity, yet at same time showing robust safety profile, making this treatment potential candidate for effective adjuvant immunotherapy. To explore possibility, we designed randomized phase II trial (EudraCT no. 2014-005123-27) to provide...
<title>Abstract</title> Next-Generation Sequencing (NGS) has transformed cancer research and clinical practice, with Whole Exome (WES) driving advances in mutational profiling personalized oncology. Yet, transcriptomic signatures remain essential for understanding disease mechanisms, including therapy resistance pathways. RNA sequencing (RNA-seq), however, faces unique challenges when dealing low-input or degraded RNA, as often found archival formalin-fixed paraffin-embedded (FFPE) tissues....
Background Chronic lymphocytic leukemia B cells display prolonged survival in vivo, but when cultured vitro rapidly undergo spontaneous apoptosis. We hypothesize that interactions with endothelial infiltrated tissues and during recirculation may have a pathogenic role chronic leukemia.Design Methods evaluated apoptosis of leukemic after co-culture on monolayer human umbilical vein addition fludarabine antibodies block adhesion. Then, we compared microarray-based gene expression profiles...
Abstract Deletion on the long arm of chromosome 11 occurs in 5–20% chronic lymphocytic leukaemia (CLL) patients. We analysed clinical–biological characteristics 131 CLL patients carrying 11q deletion documented before therapy ( de novo deleted CLL). De were characterized by high frequencies unmutated immunoglobulin variable heavy genes, multiple fluorescence situ hybridization aberrations and lymph node involvement. Factors significantly associated with shorter time to first treatment (TTFT)...
The aims of our retrospective study investigated the role immune system in glioblastoma (GBM), which is most aggressive primary brain tumor adults characterized by a poor prognosis. recurrence rate remains high, probably due to "immune-desert" microenvironment (TME) making GBM hidden from anti-tumoral clearance. Considering this, we aimed create panel prognostic markers blood and tissue correlating with overall survival (OS) progression-free (PFS).Firstly, analyzed inflammatory NLR PLR as...
Although immunomodulating antibodies are highly effective in metastatic melanoma, their toxicity, related to the activation of T lymphocytes, can be severe. Anticancer vaccines promote a fairly specific response and very well tolerated, but effectiveness has yet demonstrated. We have been treating patients with advanced melanoma an autologous dendritic cell vaccine since 2001; better characterize safety efficacy our product, we designed retrospective study on all treated date....
High-dose interleukin-2 (HD IL-2) has curative potential in metastatic melanoma (MM) and renal cell carcinoma (RCC). Radiotherapy (RT) kills cancer cells induces immunomodulatory effects. Prospective trials exploring clinical immunological properties of combined RT/HD IL-2 are still needed. We designed a phase II, single-arm trial for patients with MM RCC. The treatment schedule consisted 3 daily doses 6-12 Gy RT to 1-5 non-index fields, before at the first third cycle. HD was administered...
Background aimsDendritic cells (DCs) are professional antigen-presenting of the mammalian immune system. Ex vivo differentiated DCs represent a unique Advanced Therapy Medicinal Product (ATMP), used in several clinical trials as personalized cancer immunotherapy. The therapy's reliability depends on its capacity to produce high-quality mature (mDCs) compliance with Good Manufacturing Practices.AimsFrom March 2010 December 2023, 103 patients were enrolled multiple at Immuno-Gene Factory IRCCS...
Tumor heterogeneity is a hurdle to effective therapy, as illustrated by the 'mixed responses' frequently seen in immunotherapy-treated patients. Previously, AXL+ tumor cells were identified be highly resistant targeted whereas more differentiated MITF+ do respond RAF and MEK inhibitors. In this study, we analyzed explored presence of previously described or melanoma subpopulations metastatic tissues NanoString gene expression analysis, single-cell RNA sequencing situ multiplex...
Diet is a major driver of gut microbiota variation and plays role in metabolic disorders, including syndrome (MS). Mycorrhized foods from symbiotic agriculture (SA) exhibit improved nutritional properties, but potential benefits have never been investigated humans. We conducted pilot interventional study on 60 adults with ≥ 1 risk factors for MS, whom 33 consumed SA-derived fresh 27 received probiotics over 30 days, 15-day follow-up. Stool, urine blood were collected time to explore changes...
Advanced therapy medical products (ATMPs) are rapidly growing as innovative medicines for the treatment of several diseases. Hence, role quality analytical tests to ensure consistent product safety and has become highly relevant. Several clinical trials involving dendritic cell (DC)-based vaccines cancer ongoing at our institute. The DC-based vaccine is prepared via CD14+ monocyte differentiation. A fresh dose 10 million DCs administered patient, while remaining aliquoted, frozen, stored in...
Introduction Surgery is one of the treatments choice for patients with a single metastasis from melanoma but rarely curative. Such could potentially benefit consolidation immunotherapy. Vaccination dendritic cells (DCs) loaded tumour antigens elicits tumour-specific immune response. In our experience, who developed delayed type hypersensitivity (DTH) after DC vaccination showed median overall survival (OS) 22.9 monthsvs4.8 months DTH-negative cases. A phase II randomised trial an advantage...
Lenalidomide is a therapeutically effective drug in chronic lymphocytic leukemia (CLL). Twenty-seven CLL patients were treated with lenalidomide phase II clinical trial. Ten grouped as responders (R) and 6 nonresponders (NR). We evaluated T lymphocytes, NK, monocytes dendritic cells at baseline after treatment. A gene expression analysis was performed on 16 samples collected before The levels of immune or immune-related cytokines are not different between R NR patients. However, sensitive to...
BackgroundDendritic cells (DCs) are the most efficient antigen-presenting and act at center of immune system owing to their ability control both tolerance immunity. In cancer immunotherapy, DCs play a key role in regulation response against tumors can be generated ex vivo with different cytokine cocktails. Methods. We evaluated feasibility dinoprostone (PGE2) replacement molecular analog sulprostone, our good manufacturing practice (GMP) protocol for generation DC-based vaccine....