A5060907157- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Erythrocyte Function and Pathophysiology
- Platelet Disorders and Treatments
- Chronic Myeloid Leukemia Treatments
- Hemoglobinopathies and Related Disorders
- Acute Myeloid Leukemia Research
- Eosinophilic Disorders and Syndromes
- Hematopoietic Stem Cell Transplantation
- Kruppel-like factors research
- Blood groups and transfusion
- Epigenetics and DNA Methylation
- Cytokine Signaling Pathways and Interactions
- Mesenchymal stem cell research
- RNA modifications and cancer
- Erythropoietin and Anemia Treatment
- Cancer-related gene regulation
- Blood properties and coagulation
- Pluripotent Stem Cells Research
- Pancreatic function and diabetes
- Mast cells and histamine
- Blood transfusion and management
- Immune Cell Function and Interaction
- Multiple Myeloma Research and Treatments
- Blood disorders and treatments
- Zebrafish Biomedical Research Applications
Altius Institute for Biomedical Sciences
2021-2025
Istituto di Nanotecnologia
2024-2025
National Research Council
2024-2025
National Nanotechnology Center
2024
Institute of Nanotechnology
2024
National Academies of Sciences, Engineering, and Medicine
2024
Università Campus Bio-Medico
2021-2024
Istituto Superiore di Sanità
2007-2023
Icahn School of Medicine at Mount Sinai
2012-2023
International Society for Experimental Hematology
2018-2023
A program for banking, characterizing, and distributing placental blood, also called umbilical-cord transplantation provided grafts 562 patients between August 24, 1992, January 30, 1998. We evaluated this experience.
Clinical evidence of hematopoietic restoration with placental/umbilical cord blood (PCB) grafts indicates that PCB can be a useful source stem cells for routine bone marrow reconstitution. In the unrelated setting, human leukocyte antigen (HLA)-matched donors must obtained candidate patients and, hence, large panels frozen HLA-typed units established. The volume unprocessed units, consisting mostly red cells, plasma, and cryopreservation medium, poses serious difficulty in this effort...
Human embryonic development involves transition from yolk sac (YS) to liver (L) hemopoiesis. We report the identification of pluripotent, erythroid, and granulo-macrophage progenitors in YS, L, blood human embryos. Furthermore, comprehensive studies are presented on number hemopoietic precursors, as well other cell types, at precisely sequential stages embryos early fetuses (i.e., 4.5-8 wk 9-10 postconception, respectively). Our results provide circumstantial support a monoclonal hypothesis...
Here it is shown that the phenotype of adult mice lacking first enhancer (DNA hypersensitive site I) and distal promoter GATA-1 gene (neoΔHS or GATA-1low mutants) reveals defects in mast cell development. These include presence morphologically abnormal alcian blue+ cells apoptotic metachromatic− precursors connective tissues peritoneal lavage numerous (60–70% all progenitors) “unique” trilineage committed to erythroid, megakaryocytic, pathways bone marrow spleen. abnormalities, which were...
Recombinant rat stem cell factor (SCF) was studied for its ability to stimulate the growth of murine hematopoietic progenitor cells and generate colony-forming (CFC) from highly enriched populations cells. In serum-deprived cultures, SCF alone stimulated few colonies but interacted with a number other factors, particularly interleukin 3, promote colony formation. The most marked effect on generation mixed-cell colonies. Hematopoietic were sorted into wheat-germ agglutinin-negative,...
Abstract This study was aimed at the characterization of a gene expression signature pluripotent hematopoietic CD34+ stem cell in idiopathic myelofibrosis (IM), which would eventually provide novel pathogenetic insights and/or diagnostic/prognostic information. Aberrantly regulated genes were revealed by transcriptome comparative microarray analysis normal and IM cells; selected also assayed granulocytes. One-hundred seventy four differentially expressed identified part validated...
Lysyl oxidase (LOX), a matrix cross-linking protein, is known to be selectively expressed and enhance fibrotic phenotype. A recent study of ours showed that LOX oxidizes the PDGF receptor-β (PDGFR-β), leading amplified downstream signaling. Here, we examined expression functions in megakaryocytes (MKs), platelet precursors. Cells committed MK lineage undergo mitotic proliferation yield diploid cells, followed by endomitosis acquisition polyploidy. Intriguingly, detected diploid-tetraploid...
Primary myelofibrosis (PMF) is a clonal hematologic malignancy characterized by BM fibrosis, extramedullary hematopoiesis, circulating CD34+ cells, splenomegaly, and propensity to evolve acute myeloid leukemia. Moreover, the spleen of patients harbor atypical, clustered megakaryocytes, which contribute disease secreting profibrotic cytokines. Here, we have revealed that megakaryocytes in PMF show impaired maturation associated with reduced GATA1 protein. In investigating cause...
Myelofibrosis (MF) is a progressive chronic myeloproliferative neoplasm characterized by hyperactivation of JAK/STAT signaling and dysregulation the transcription factor GATA1 in megakaryocytes (MKs). TGF-β plays pivotal role pathobiology MF promoting BM fibrosis collagen deposition enhancing dormancy normal hematopoietic stem cells (HSCs). In this study, we show that MF-MKs elaborated significantly greater levels TGF-β1 than TGF-β2 TGF-β3 to varying degree, evaluated ability AVID200, potent...
Rubinstein, Pablo; Carrier, Carmelita; Scaradavou, Andromachi; Kurtzberg, Joanne; Adamson, John; Migliaccio, Anna Rita; Berkowitz, Richard L.; Cabbad, Michael; Dobrila, N. Ludy; Taylor, Patricia E.; Rosenfield, Stevens, Cladd E. Author Information
Erythropoietin (Epo), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte factor- (G-CSF) dependent cell lines have been derived from the murine hematopoietic line 32D with a selection strategy involving culture of cells in FBS-deprived medium supplemented only pure recombinant Epo, GM-CSF, or G-CSF. The retain diploid karyotype original clone, do not grow absence exogenous growth factor, induce tumors when injected into syngeneic recipients. morphology Epo-dependent...
Ex vivo-generated erythroblasts represent alternative transfusion products. However, inclusion of bovine components in media used for their growth precludes clinical use, highlighting the importance developing culture based on pharmaceutical grade reagents. In addition, because adult blood generates ex vivo lower numbers than cord blood, has been proposed as source choice erythroblast production. To clarify potential to generate vivo, experiments were designed identify factors [stem cell...
Expansion of erythroblasts from human peripheral blood mononuclear cells is 4- to 15-fold more efficient than that CD34+ purified cells. In addition, and CD34− populations do not reconstitute this erythroid yield, suggesting a role for feeder present in increase hematopoietic output. Immunodepleting CD14+ reduced stem progenitor cell expansion. Conversely, the yield was increased upon co-culture with (full contact or transwell assays) re-constituted conditioned medium particular, CD14++CD16+...