A5085053942- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Tuberculosis Research and Epidemiology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Antibiotics Pharmacokinetics and Efficacy
- Gene expression and cancer classification
- Metabolomics and Mass Spectrometry Studies
- Microbial Natural Products and Biosynthesis
- Pneumonia and Respiratory Infections
- Genomics and Phylogenetic Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial Genetics and Biotechnology
- Mycobacterium research and diagnosis
McMaster University
2018-2022
The Comprehensive Antibiotic Resistance Database (CARD; https://card.mcmaster.ca) is a curated resource providing reference DNA and protein sequences, detection models bioinformatics tools on the molecular basis of bacterial antimicrobial resistance (AMR). CARD focuses high-quality data sequences within controlled vocabulary, Ontology (ARO), designed by biocuration team to integrate with software development efforts for resistome analysis prediction, such as CARD's Gene Identifier (RGI)...
Abstract The Comprehensive Antibiotic Resistance Database (CARD; card.mcmaster.ca) combines the Ontology (ARO) with curated AMR gene (ARG) sequences and resistance-conferring mutations to provide an informatics framework for annotation interpretation of resistomes. As version 3.2.4, CARD encompasses 6627 ontology terms, 5010 reference sequences, 1933 mutations, 3004 publications, 5057 detection models that can be used by accompanying Gene Identifier (RGI) software annotate genomic or...
Lipid A phosphoethanolamine (PEtN) transferases render bacteria resistant to the last resort antibiotic colistin. The recent discoveries of pathogenic harboring plasmid-borne PEtN transferase (mcr) genes have illustrated serious potential for wide dissemination these resistance elements. origin mcr-1 is traced Moraxella species co-occupying environmental niches with Enterobacteriaceae. Here, we describe crystal structure catalytic domain chromosomally encoded colistin transferase, ICRMc (for...
Diagnosing antimicrobial resistance (AMR) in the clinic is based on empirical evidence and current gold standard laboratory phenotypic methods. Genotypic methods have potential advantages of being faster cheaper, having improved mechanistic resolution over We generated applied rule-based logistic regression models to predict AMR phenotype from Escherichia coli Pseudomonas aeruginosa multidrug-resistant clinical isolate genomes. By inspecting evaluating these models, we identified previously...
One of the main challenges in search for new antibiotics from natural product extracts is re-discovery common compounds. To address this challenge, dereplication, which process identifying known compounds, performed on samples interest. Methods dereplication such as analytical separation followed by mass spectrometry are time-consuming and resource-intensive. improve process, we have developed antibiotic resistance platform (ARP). The ARP a library approximately 100 genes that been...
One of the main challenges in search for new antibiotics from natural product extracts is re-discovery common compounds. To address this challenge, dereplication, which process identifying known compounds, performed on samples interest. Methods dereplication such as analytical separation followed by mass spectrometry are time-consuming and resource-intensive. improve process, we have developed antibiotic resistance platform (ARP). The ARP a library approximately 100 genes that been...