A5041952327- Microbial Natural Products and Biosynthesis
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Fluid Dynamics and Turbulent Flows
- Turbomachinery Performance and Optimization
- Crystallization and Solubility Studies
- Aerodynamics and Fluid Dynamics Research
- Computational Drug Discovery Methods
- X-ray Diffraction in Crystallography
- Wind and Air Flow Studies
- Assisted Reproductive Technology and Twin Pregnancy
- Molecular spectroscopy and chirality
- Plant and fungal interactions
- Metabolomics and Mass Spectrometry Studies
- Reproductive Health and Technologies
- Natural product bioactivities and synthesis
- Plant biochemistry and biosynthesis
- Microbial Metabolism and Applications
- Ovarian function and disorders
- Pharmacogenetics and Drug Metabolism
- Infection Control and Ventilation
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Analytical Chemistry and Chromatography
- Antibiotic Resistance in Bacteria
Health Canada
2021-2025
Kinokuniya
2019
Cambridge University Press
2019
New York University Press
2019
QIMR Berghofer Medical Research Institute
2019
Hospital Universitário da Universidade de São Paulo
2019
McMaster University
2012-2018
Carleton University
2006-2016
University of Copenhagen
1999-2013
Merck Canada Inc. (Canada)
2009-2011
ABSTRACT Cyclic lipopeptides (CLPs) with antibiotic and biosurfactant properties are produced by a number of soil bacteria, including fluorescent Pseudomonas spp. To provide new efficient strains for the biological control root-pathogenic fungi in agricultural crops, we isolated approximately 600 from two different soils using three growth media. CLP production was observed large proportion (approximately 60%) inhabiting sandy soil, compared to low 6%) loamy soil. Chemical structure analysis...
Computational fluid dynamics (CFD) is used routinely to predict air movement and distributions of temperature concentrations in indoor environments. Modelling numerical errors are inherent such studies must be considered when the results presented. Here, we discuss modelling aspects turbulence boundary conditions, as well related errors, with emphasis on choice differencing scheme computational grid. Illustrative examples given stress main points errors. Finally, recommendations for...
Infertility is a couple-based fecundity impairment, although population level research largely based upon information reported by female partners. Of the few studies focusing on male partners, most focus utilization of infertility services rather than efforts to estimate prevalence and determinants as Data from nationally representative sample men aged 15–44 years who participated in 2002 National Survey Family Growth (NSFG) were used longer time-to-pregnancy (TTP) using novel current...
The potential use of SCD inhibitors for the chronic treatment diabetes and dyslipidemia has been limited by preclinical adverse events associated with inhibition in skin eye tissues. To establish a therapeutic window, we embarked on designing liver-targeted utilizing molecular recognition liver-specific organic anion transporting polypeptides (OATPs). In doing so, set out to target inhibitor organ believed be responsible efficacy (liver) while minimizing its exposure tissues mechanism-based...
Assessing couple fecundity on a nation-wide basis without excluding couples who eventually remain infertile is challenging. Our aim was to describe (in terms of frequency involuntary infertility) among the general population living in France. We used current-duration design. A random sample 64 262 households selected 2007–2008, allowing us identify 15 810 women aged 18–44 years. Eligible (n= 1089) were those having regular sexual intercourse with male partner, not using any method...
Pseudomonas sp. strain DSS73 isolated from the sugar beet rhizosphere produces cyclic lipopeptide amphisin, which inhibits growth of plant-pathogenic fungi. By Tn5::luxAB mutagenesis, we obtained two nonproducing mutant strains, DSS73-15C2 and DSS73-12H8. The gene interrupted by transposon in (amsY) encoded a protein with homology to peptide synthetases that was designated amphisin synthetase. DSS73-12H8 carried regulatory encoding sensor kinase GacS. Growth impaired during transition...
Most existing antibiotics were discovered through screens of environmental microbes, particularly the streptomycetes, for capacity to prevent growth pathogenic bacteria. This "activity-guided screening" method has been largely abandoned because it repeatedly rediscovers those compounds that are highly expressed during laboratory culture. these metabolites have already biochemically characterized. However, sequencing streptomycete genomes revealed a large number "cryptic" secondary metabolic...
The crystal structure of the lipoundecapeptide amphisin, presented here as tetrahydrate, C66H114N12O20·4H2O, originating from non-ribosomal biosynthesis by Pseudomonas sp. strain DSS73, has been solved to a resolution 0.65 Å. primary amphisin is β-hydroxydecanoyl-d-Leu-d-Asp-d-allo-Thr-d-Leu-d-Leu-d-Ser-l-Leu-d-Gln-l-Leu-l-Ile-l-Asp (Leu leucine, Asp aspartic acid, Thr threonine, Ser serine, Gln glutamine and Ile isoleucine). peptide lactone, linking Thr4 Oγ C-terminal. stereochemistry...
The sequence positions of d and l Leu Lys residues in bogorol A (1) have been defined by a simple novel approach that utilizes small amounts sample focuses on detecting the order which amino acids are liberated from parent peptide during acid-catalyzed hydrolysis. This technique builds previously established relationship between steric electronic features their predilection for acidic liberation polypeptides via dipeptides. results, complete structure A, confirmed traditional degradation...
X-hitting, a newly developed algorithm for automated comparison of UV data, has been used the tracking two novel spiro-quinazoline metabolites, lapatins A (1) and B (2), in screening study targeting quinazolines. The structures 1 2 were elucidated by analysis spectroscopic primarily 2D NMR.
Three eremophilane sesquiterpenes (1, 2, and 3) were isolated from Penicillium roqueforti DAOM 232127, their structures established. The new (3S)-3-acetoxyeremophil-1(2),7(11),9(10)-trien-8-one (3) is a likely biosynthetic precursor of PR toxin. 1-Hydroxyeremophil-7(11),9(10)-dien-8-one (1) related to the immunosuppressant cuspidatol. application semihyphenated LC-MS-SPE/NMR rapidly identify, purify, elucidate 1, 3 described.