A5015749727- Immunotherapy and Immune Responses
- Nanoparticle-Based Drug Delivery
- Mosquito-borne diseases and control
- RNA Interference and Gene Delivery
- HIV Research and Treatment
- Nanoplatforms for cancer theranostics
- Chemokine receptors and signaling
- Viral Infections and Vectors
- Immune Cell Function and Interaction
- Viral Infections and Outbreaks Research
- Advanced biosensing and bioanalysis techniques
- Advancements in Transdermal Drug Delivery
- Virology and Viral Diseases
- Cancer Immunotherapy and Biomarkers
- Bacteriophages and microbial interactions
- Dermatology and Skin Diseases
- Ocular Surface and Contact Lens
- vaccines and immunoinformatics approaches
- Dendrimers and Hyperbranched Polymers
- 3D Printing in Biomedical Research
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Malaria Research and Control
- Polymer Surface Interaction Studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
University of North Carolina at Chapel Hill
2016-2025
UNC Lineberger Comprehensive Cancer Center
2012-2016
Bipar
2007-2015
University of Illinois Urbana-Champaign
2002-2012
Kettering University
2010
North Carolina State University
2010
Memorial Sloan Kettering Cancer Center
2010
nLIGHT (United States)
2005
Harvard University
2005
Dana-Farber Cancer Institute
2005
Transferrin receptor (TfR, CD71) has long been a therapeutic target due to its overexpression in many malignant tissues. In this study, PRINT nanoparticles were conjugated with TfR ligands for targeted drug delivery. Cylindrical poly(ethylene glycol)-based (diameter (d) = 200 nm, height (h) nm) labeled transferrin antibody (NP-OKT9) or human (NP-hTf) showed highly specific TfR-mediated uptake by all tumor cell lines tested, relative negative controls (IgG1 OKT9 bovine (bTf) hTf). The...
Vaccination is an essential public health measure for infectious disease prevention. The exposure of the immune system to vaccine formulations with appropriate kinetics critical inducing protective immunity. In this work, faceted microneedle arrays were designed and fabricated utilizing a three-dimensional (3D)-printing technique called continuous liquid interface production (CLIP). design resulted in increased surface area as compared smooth square pyramidal design, ultimately leading...
Nanotechnology can provide a critical advantage in developing strategies for cancer management and treatment by helping to improve the safety efficacy of novel therapeutic delivery vehicles. This paper reports fabrication poly(lactic acid-co-glycolic acid)/siRNA nanoparticles coated with lipids use as prostate therapeutics made via unique soft lithography particle molding process called Particle Replication In Nonwetting Templates (PRINT). The PRINT enables high encapsulation efficiency...
A critical need still remains for effective delivery of RNA interference (RNAi) therapeutics to target tissues and cells. Self-assembled lipid- polymer-based systems have been most extensively explored transfection with small interfering (siRNA) in liver cancer therapies. Safety compatibility materials implemented must be ensured maximize therapeutic indices. Hydrogel nanoparticles defined dimensions compositions, prepared via a particle molding process that is unique off-shoot soft...
Nanoparticle delivery of subunit vaccines may increase vaccine efficacy, leading to a wide variety safe and effective beyond those available through dosing inactivated or live, attenuated whole pathogens. Here we present versatile platform based on PRINT hydrogels made biocompatible hydroxy-poly(ethylene glycol) (PEG) that is able activate the complement system by alternative pathway. These lymph node targeting nanoparticles (NPs) promote immunogenicity model antigen, ovalbumin, showing...
Tumor evasion of immune destruction is associated with the production immunosuppressive adenosine in tumor microenvironment (TME). Anticancer therapies can trigger triphosphate (ATP) release from cells, causing rapid formation by ectonucleotidases CD39 and CD73, thereafter exacerbating immunosuppression TME. The goal this study was to develop an approach facilitate cancer therapy–induced immunogenic cell death including ATP limit degradation into adenosine, order achieve durable antitumor...
Binding of peptide/MHC (pMHC) complexes by TCR initiates T cell activation. Despite long interest, the exact relationship between biochemistry TCR/pMHC interaction (particularly affinity or ligand off-rate) and responses remains unresolved, because number examined in each independent system has been too small to draw a definitive conclusion. To test current models activation, we have analyzed interactions mouse P14 set altered peptides based on lymphocytic choriomeningitis virus epitope...
Abstract Using high‐resolution 3D printing, a novel class of microneedle array patches (MAPs) is introduced, called latticed MAPs (L‐MAPs). Unlike most which are composed either solid structures or hollow needles, L‐MAPs incorporate tapered struts that form cells capable trapping liquid droplets. The lattice can also be coated with traditional viscous coating formulations, enabling both liquid‐ and solid‐state cargo delivery, on single patch. Here, library 43 L‐MAP designs generated...
Herein, we report the fabrication of protein (bovine serum albumin, BSA) particles which were rendered transiently insoluble using a novel, reductively labile disulfide-based cross-linker. After being cross-linked, retain their integrity in aqueous solution and dissolve preferentially under reducing environment. Our data demonstrates that cleavage cross-linker leaves no chemical residue on reactive amino group. Delivery self-replicating RNA was achieved via PRINT particles. These can provide...
CpG oligodeoxynucleotides are potent toll-like receptor (TLR) 9 agonists and have shown promise as anticancer agents in preclinical studies clinical trials. Binding of to TLR9 initiates a cascade innate adaptive immune responses, beginning with activation dendritic cells resulting range secondary effects that include the secretion pro-inflammatory cytokines, natural killer cells, expansion T cell populations. Recent literature suggests local delivery tumors results superior antitumor...
A stabilized dimer of the surface protein from dengue virus has been engineered to elicit antibodies that neutralize virus.
Abstract Personalized biomedical devices, such as microneedle array patches (MAPs), offer a promising transdermal drug delivery technology, providing safe, painless, and self- administered alternative to traditional hypodermic injections. Despite their potential for precise therapeutic release, MAP adoption has been limited by challenges in payload capacity, treatment versatility, manufacturing scalability. To address these issues, we integrated microfluidic channel designs with enhancing...
The entry of human immunodeficiency virus type 1 (HIV-1) into the cell is initiated by interaction viral surface envelope protein with two components target cell, CD4 and a chemokine coreceptor, usually CXCR4 or CCR5. natural ligand stromal cell-derived factor 1alpha (SDF-1alpha). Whereas overlap between HIV-1 SDF-1alpha functional sites on extracellular domains has been well documented, it yet to be determined whether there are in transmembrane (TM) helices important for and/or functions,...
Lowering the modulus of hydrogel particles could enable them to bypass in vivo physical barriers that would otherwise filter with similar size but higher modulus. Incorporation electrolyte moieties into polymer network increase swelling ratio is a straightforward and quite efficient way decrease In addition, charged groups can also help secure cargoes. However, distribution on surface particle accelerate clearance particles. Herein, we developed method synthesize highly swollen microgels...
The chemokine receptor CXCR4 plays an important role as the for normal physiological function of stromal cell-derived factor 1alpha (SDF-1alpha) and coreceptor entry human immunodeficiency virus type 1 (HIV-1) into cell. In a recent work (S. Tian et al., J. Virol. 79:12667-12673, 2005), we found that many residues throughout transmembrane (TM) extracellular loop 2 domains are specifically involved in interaction with HIV-1 gp120, most these sites did not play either SDF-1alpha binding or...
Herein we report the development of a nonviral lipid-complexed PRINT (particle replication in nonwetting templates) protein particle system (LPP particle) for RNA replicon delivery with view toward replicon-based vaccination. Cylindrical bovine serum albumin (BSA) particles (diameter (d) 1 μm, height (h) μm) loaded and stabilized fully reversible disulfide cross-linker were fabricated using technology. Highly efficient to Vero cells was achieved by complexing mixture...
Educating our immune system via vaccination is an attractive approach to combat infectious diseases. Eliciting antigen specific cytotoxic T cells (CTLs), CD8+ effector cells, essential in controlling intracellular diseases such as influenza (Flu), tuberculosis (TB), hepatitis, and HIV/AIDS, well tumors. However, utilizing subunit peptides elicit a potent cell response with antigenic typically ineffective due poor immunogenicity. Here we have engineered reduction sensitive nanoparticle (NP)...
Dengue virus (DENV) is the causative agent of dengue fever and hemorrhagic fever. The endemic in over 120 countries, causing 350 million infections per year. vaccine development challenging because need to induce simultaneous protection against four antigenically distinct DENV serotypes evidence that, under some conditions, vaccination can enhance disease due specific immunity virus. While several live-attenuated tetravalent vaccines display partial efficacy, it has been balanced protective...