Alfin1 cDNA encodes a putative transcription factor associated with NaCl tolerance in alfalfa (Medicago sativa L.). The recombinant protein binds DNA sequence-specific manner, including promoter fragments of the NaCl-inducible gene MsPRP2. function was tested transgenic under control 35S sense and antisense orientations endogenous MsPRP2 as reporter gene. Calli overexpressing were more resistant to growth inhibition by 171 mM than vector-transformed controls, whereas calli expressing...

Telehealth nursing, or the delivery, management, and coordination of nursing care services provided via telecommunications technology, is one methods delivering health to patients in United States. It important assess service quality involved professionals as well telehealth process. The focus this study innovative model delivery by nurses for managing with chronic disease while they are living their own residence.The primary objective pilot was examine whether technology impacts perceived...

Carrot (Daucus carota L.) cells were transformed with Agrobacterium tumefaciens strains containing 3[prime]-truncated mouse ornithine decarboxylase (ODC) cDNA under the control of a cauliflower mosaic virus 35S promoter. A neomycin phosphotransferase gene linked nopaline synthase promoter was used to select cell lines on kanamycin. Although nontransformed contained no ODC, high amounts mouse-specific ODC activity observed in cells. Transgenic showed significant increase cellular content...

Regulation of cellular proliferation and differentiation during brain development results from processes requiring several regulatory networks to function in synchrony. MicroRNAs are part this system. Although many microRNAs evolutionarily conserved, recent evolution such molecules can enable the acquisition new means attaining specialized functions. Here we identify report novel expression functions a human higher primate-specific microRNA, miR-1290, neurons. Using fetal-derived neural...

<b><i>Aim:</i></b> The aim of this study was to evaluate current direct-to-consumer (DTC) genetic customers' ability interpret and comprehend test results determine if honest brokers are needed<i>. </i><b><i>Method:</i></b> One hundred twenty-two customers the DTC testing company 23andMe were polled in an online survey. subjects asked about their personal two mock cases (type 2 diabetes multiple sclerosis), where translated into...

The metabolisms of arginine (Arg), ornithine (Orn), and putrescine were compared in a nontransgenic transgenic cell line carrot (Daucus carota L.) expressing mouse Orn decarboxylase cDNA. [14C]Arg, [14C]Orn, [14C]putrescine fed to cells their rates decarboxylation, uptake, metabolism into polyamines, incorporation acid-insoluble material determined. Transgenic showed higher decarboxylation for labeled than the cells. This was correlated positively with amounts production from Orn. With Arg,...

We previously showed that mixing transferrin with a cationic liposome prior to the addition of DNA, greatly enhanced lipofection efficiency. Here, we report characterization transfection complexes in formulations prepared transferrin, lipofectin, and DNA (pCMVlacZ) various formulations. all contain lipofectin was resistant DNase I treatment. Transfection experiments performed Panc 1 cells standard formulation, which by adding mixture yielded highest There no apparent difference zeta...

The rapid and reliable identification of clinically significant Mycobacterium species is a challenge for diagnostic laboratories. This study evaluates unique sequence-dependent algorithm called MycoAlign the differential species. system uses pan-Mycobacterium-specific primer amplification in combination with customized database algorithm. results testing were compared conventional phenotypic assays GenBank sequence comparisons using 16S rRNA target. Discrepant retested evaluated third...

Vitamin A and the T helper 2 cytokines IL-4 IL-13 play important roles in induction of mucin gene expression mucus hypersecretion. However, effects these agents on enzymes responsible for glycosylation have received little attention. Here, we report upregulation core beta1,6 N-acetylglucosaminyltransferase (C2GnT) activity both by all-trans retinoic acid (RA) H292 airway epithelial cell line. Northern blotting analysis showed that M isoform C2GnT, which is expressed mucus-secreting tissues...

The goal of this study was to apply temperature-mediated heteroduplex analysis using denaturing high-performance liquid chromatography identify pyrazinamide (PZA) resistance in Mycobacterium tuberculosis isolates and simultaneously differentiate between M. bovis. Features that contributed an optimal assay included the use two different reference probes for pncA gene targets from wild-type bovis, optimization column temperature, increasing starting concentration elution buffer, reducing rate...

The integration of EHR in IT infrastructures supporting organizations enable improved access and recording patient data, enhanced ability to make decisions, quality reduced errors care. Despite these benefits, there are mixed results as the use EHR. literature suggests that reasons for limited relate policy, financial usability considerations, but it does not provide an understanding physicians' interaction adaptation Following analysis qualitative collected a case study at hospital using...

Enzymes which exhibit core 2 beta1,6 N-acetylglucosaminyltransferase (C2GnT) activity play important roles in physiologic processes including the inflammatory response and immune system function, C2GnT is regulated during processes, such as T cell activation cellular differentiation. In this study, we have examined regulation of H292 airway epithelial line by epidermal growth factor (EGF), has been previously shown to upregulate expression mucin MUC5AC line. We found that EGF suppressed a...

Glycemic variability (GV) is an obstacle to effective blood glucose control and autonomous risk factor for diabetes complications. We, therefore, explored sample data of patients with mellitus who maintained better amplitude glycemic fluctuations compared their disease outcomes groups having poor control. A retrospective study was conducted using electronic hemoglobin A1C (HbA1c) values five recent time points from Think Whole Person Healthcare (TWPH). The grid analysis (CVGA) plot...

Network pharmacology is an emerging approach for establishing the "compounds-proteins/genes-diseases" network, aiding in drug target prediction through interactions between small molecules and host microbes. In recent years, considerable information on biochemical properties, location biological properties of herbal phytochemicals their with microbiome have been accumulated made available as repositories accessible to general public. However, scattered data from thousands experiments,...