A5054984640- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Virus-based gene therapy research
- Nanoplatforms for cancer theranostics
- Cancer Immunotherapy and Biomarkers
- MicroRNA in disease regulation
- Hepatitis B Virus Studies
- Cancer Research and Treatments
- Circular RNAs in diseases
- ATP Synthase and ATPases Research
- Advanced Nanomaterials in Catalysis
- Orthopedic Surgery and Rehabilitation
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- interferon and immune responses
- Immune Cell Function and Interaction
- Cancer Mechanisms and Therapy
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
Precision BioSciences (United States)
2022
University of North Carolina at Chapel Hill
2014-2020
Pharmac
2017
Institute of Nanotechnology
2016
CeNTech
2016
American Pharmacists Association
2016
University of North Carolina Wilmington
2016
The University of Texas at Austin
1982
Although great success has been obtained in the clinic, current immune checkpoint inhibitors still face two challenging problems: low response rate and immune-related adverse effects (irAEs). Here we report combination of immunogenic chemotherapy locally expressed PD-L1 trap fusion protein for efficacious safe cancer immunotherapy. We demonstrate that oxaliplatin (OxP) boosts anti-PD-L1 mAb therapy against murine colorectal cancer. By design a loading its coding plasmid DNA into...
Our previous work demonstrated that Wnt16 expression in cisplatin-damaged tumor-associated fibroblasts is a key factor contributing to cisplatin resistance malignancies. Natural antifibrotic compounds with low toxicities are promising candidates downregulate expression, improving the antitumor effect of nanoparticles. Upon screening several natural chemicals, we found dietary flavonoid, quercetin, significantly suppresses activated fibroblasts. To facilitate drug delivery, have prepared...
Objective Myeloid-derived suppressor cells (MDSCs) contribute to tumour immunosuppressive microenvironment and immune-checkpoint blockade resistance. Emerging evidence highlights the pivotal functions of cyclin-dependent kinases (CDKs) in immunity. Here we elucidated role tumour-intrinsic CDK20, or cell cycle-related kinase (CCRK) on immunosuppression hepatocellular carcinoma (HCC). Design Immunosuppression MDSCs derived from patients with HCC relationship CCRK were determined by flow...
The conjugate of paclitaxel (PTX) and docosahexaenoic acid has entered into clinical trials. However, the most recent outcomes fell short expectations, due to extremely slow drug release from hydrophobic conjugates. Herein, a novel prodrug-based nanoplatform self-assembled by disulfide bond linked conjugates PTX oleic for rapid differential in tumor cells is reported. This redox-responsive prodrug-nanosystem demonstrates multiple therapeutic advantages, including one-step facile fabrication,...
Pancreatic tumors are known to be resistant immunotherapy due the extensive immune suppressive tumor microenvironment (TME). We hypothesized that CXCL12 and PD-L1 two key molecules controlling immunosuppressive TME. Fusion proteins, called traps, designed bind with these high affinity (Kd = 4.1 0.22 nM, respectively) were manufactured tested for specific binding targets. Plasmid DNA encoding each trap was formulated in nanoparticles intravenously injected mice bearing orthotopic pancreatic...
In many cancers, the tumor microenvironment (TME) is largely immune suppressive, blocking antitumor immunity and resulting in immunotherapy resistance. Interleukin 10 (IL-10) a major player controlling immunosuppressive TME different murine models. Increased IL-10 production suppresses intratumoral dendritic cell of interleukin 12, thereby limiting cytotoxic T-cell responses activation NK cells during therapy. We engineered, formulated, delivered genes encoding an protein trap to change TME,...
Targeted nanoparticles induce expression of a CXCL12/SDF-1 trap in transient and liver-specific manner to inhibit colorectal cancer liver metastasis.
Persistence of chronic hepatitis B (CHB) is attributed to maintenance the intrahepatic pool viral covalently closed circular DNA (cccDNA), which serves as transcriptional template for all gene products required replication. Current nucleos(t)ide therapies CHB prevent virus production and spread but have no direct impact on cccDNA or expression genes. We describe a potential curative approach using highly specific engineered ARCUS nuclease (ARCUS-POL) targeting (HBV) genome. Transient...
Submission Type: Hallux Valgus Research Level 2 - Prospective comparative study, Meta-analysis of studies or 1 with inconsistent results Introduction/Purpose: The medial closing wedge osteotomy the proximal phalanx, known as Akin osteotomy, is a common procedure in combination metatarsal osteotomies and soft tissue realignment procedures to achieve hallux valgus deformity correction. With developments surgical instrumentation, option perform percutaneously has grown popularity. There limited...
Abstract Lung squamous carcinoma (LUSC) is a highly metastatic disease with poor prognosis. Using an integrated screening approach, we found that miR-671-5p reduces LUSC metastasis by inhibiting circular RNA (circRNA), CDR1as. Although the putative function of circRNA through miRNA sponging, more potently silenced axis CDR1as and its antisense transcript, cerebellar degeneration related protein 1 (CDR1). Silencing or CDR1 significantly inhibited metastases was sufficient to promote migration...
DNA-alkylating drugs continue to remain an important weapon in the arsenal against cancers. However, they typically suffer from several shortcomings because of indiscriminate DNA damage that cause and their inability specifically target cancer cells. We have developed a strategy for overcoming deficiencies current chemotherapy by designing site-specific DNA-methylating agent can cells its selective uptake via glucose transporters, which are overexpressed most The design features molecule,...
Liposomes bearing human tumor membrane vesicles were effective immunogenic complexes for inducing antibodies in rabbits. Multilamellar liposomes (MLV) at 7:4:1 molar ratios of phosphatidylcholine, cholesterol and phosphatidic acid prepared with sonicated (MN) isolated from LS174T colon cells. MLV together MN (i.e., MN-MLV antigens) or added to preformed MN+MLV antigens), antigens alone used as immunogens. Rabbits immunized i.v. 100 g protein each antigen group. Boosters (i.v.) days 13 29....
Abstract The tumor microenvironment plays an instrumental role in cancer development and treatment response/resistance. Accumulating evidence is underscoring the fundamental importance of epigenetic regulation immune evasion. Epigenetic modification agents represent a ‘double-edged sword’, as they can exert divergent effects on cells milieu stromal cells. Our integrative epigenomics analysis has elucidated previously unexplored functions histone deacetylase 8 (HDAC8) promoting...
<div>Abstract<p>Lung squamous carcinoma (LUSC) is a highly metastatic disease with poor prognosis. Using an integrated screening approach, we found that miR-671-5p reduces LUSC metastasis by inhibiting circular RNA (circRNA), CDR1as. Although the putative function of circRNA through miRNA sponging, more potently silenced axis CDR1as and its antisense transcript, cerebellar degeneration related protein 1 (CDR1). Silencing or CDR1 significantly inhibited metastases was sufficient...
<div>Abstract<p>Lung squamous carcinoma (LUSC) is a highly metastatic disease with poor prognosis. Using an integrated screening approach, we found that miR-671-5p reduces LUSC metastasis by inhibiting circular RNA (circRNA), CDR1as. Although the putative function of circRNA through miRNA sponging, more potently silenced axis CDR1as and its antisense transcript, cerebellar degeneration related protein 1 (CDR1). Silencing or CDR1 significantly inhibited metastases was sufficient...
<p>Time lapse imaging of RUSH assay in SK-MES-1-EV cells transfected with Ii-Str_ssSBP-EGFP (green) and Golgi marker mApple-SiT (magenta) beginning 5 min after biotin addition.</p>
<p>Candidate target genes for miR-671-5p, values based on median expression of miR-671-5p and candidate genes.</p>
<p>Expression of miRs in SK-MES-1 and LN1 measured by Nanostring with normalized counts greater than 5 at least one the two compared samples.</p>