A5038896281- Genetics, Aging, and Longevity in Model Organisms
- Bone Tissue Engineering Materials
- Reproductive Biology and Fertility
- Neurobiology and Insect Physiology Research
- Developmental Biology and Gene Regulation
- Insect and Arachnid Ecology and Behavior
- Circadian rhythm and melatonin
- Orthopaedic implants and arthroplasty
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Cellular Mechanics and Interactions
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Spaceflight effects on biology
- Nematode management and characterization studies
- Supramolecular Self-Assembly in Materials
- Advanced Chemical Sensor Technologies
- Olfactory and Sensory Function Studies
- Reproductive System and Pregnancy
- 3D Printing in Biomedical Research
- Neonatal Respiratory Health Research
- Insect Pheromone Research and Control
Hong Kong University of Science and Technology
2013-2025
University of Hong Kong
2013-2025
Chinese University of Hong Kong
2021
Prince of Wales Hospital
2021
Western University
2008
Bioengineering Center
2008
Albert Einstein College of Medicine
1991-1995
Baylor College of Medicine
1990-1992
Texas Medical Center
1991
Yeshiva University
1991
Abstract Osteoinductivity of hydroxyapatite (HA) was investigated using uncommitted pluripotent mouse stem cells, C3H10T1/2 in an vitro differentiation assay. For comparative analysis, the cells were cultured on substrates made osteoinductive HA, with biocompatible titanium and plastics as negative control. HA exhibited ability to induce expression osteo‐specific genes C3H10T1/2, including alkaline phosphatase (ALP), type I collagen, osteocalcin; compared its insignificant up‐regulation same...
Objective Myeloid-derived suppressor cells (MDSCs) contribute to tumour immunosuppressive microenvironment and immune-checkpoint blockade resistance. Emerging evidence highlights the pivotal functions of cyclin-dependent kinases (CDKs) in immunity. Here we elucidated role tumour-intrinsic CDK20, or cell cycle-related kinase (CCRK) on immunosuppression hepatocellular carcinoma (HCC). Design Immunosuppression MDSCs derived from patients with HCC relationship CCRK were determined by flow...
Myocardial infarction (MI) is a medical emergency for which the early detection of symptoms desirable. The prevalence portable electrocardiogram (ECG) devices makes frequent screening MI possible. In this study, we develop an classifier that combines both convolutional and recurrent neural networks, suitable wearable ECG with only single lead recording. It performs multiclass classification to discriminate records from those healthy individuals patients existing chronic heart conditions, as...
ABSTRACT We have identified a new member of the TGF-β superfamily, CET-1, from Caenorhabditis elegans, which is expressed in ventral nerve cord and other neurons. cet-1 null mutants shortened bodies male tail abnormal phenotype resembling sma mutants, suggesting cet-1, sma-2, sma-3 sma-4 share common pathway. Overexpression experiments demonstrated that function requires wild-type genes. Interestingly, CET-1 appears to affect body length dose-dependent manner. Heterozygotes for displayed...
Three upstream CBAR cis-acting promoter elements, containing the inner core CC(A/T)6GG of serum response element (SRE), are required for myogenic cell type-restricted expression avian skeletal alpha-actin gene (K.L. Chow and R.J. Schwartz, Mol. Cell. Biol. 10:528-538, 1990). These actin SRE elements display differential binding properties with two distinct nuclear proteins, factor (SRF) another described here as F-ACT1. SRF is able to bind all SREs various affinities. This multisite...
Common dehydration methods of cells on biomaterials for scanning electron microscopy (SEM) include air drying, hexamethyldisilazane (HMDS) or tetramethysilane (TMS) treatment and critical point drying (CPD). On the other side, freeze-drying has been widely employed in dehydrating biological samples also preparing porous biomaterial scaffolds but not three-dimensional (3D) SEM examination. In this study, we compare hydroxyapatite (HA) prepared by HMDS freeze-drying. The effects fixation using...
Abstract The free-living nematode worm Caenorhabditis elegans reproduces primarily as a self-fertilizing hermaphrodite, yet males are maintained in wild-type populations at low frequency. To determine the role of C. elegans, we develop mathematical model for genetic system hermaphrodites that can either self-fertilize or be fertilized by and perform laboratory observations experiments on both related dioecious species remanei. We show mating efficiency is poor compared to more attracted...
In cardiac muscle, acidic and basic fibroblast growth factors (aFGF bFGF) regulate at least five genes in common (including alpha beta myosin heavy chains, atrial natriuretic factor, the sarcoplasmic reticulum calcium ATPase), provoking a generalized "fetal" phenotype similar to events pressure-overload hypertrophy; however, aFGF bFGF differentially control striated alpha-actins. stimulates inhibits skeletal alpha-actin transcripts associated with embryonic heart, whereas mRNA is inhibited...
ABSTRACT The gene mab-21, which encodes a novel protein of 386 amino acids, is required for the choice alternate cell fates by several cells in C. elegans male tail. Three descended from ray 6 precursor adopt anterior homologs, and fourth, lineally unrelated hypodermal transformed into neuroblast. affected lie together lateral tail epidermis, suggesting that mab-21 acts as part short-range pattern-formation mechanism. Each changes fate brought about mutants can be interpreted...
ABSTRACT The copulatory structure of the C. elegans male tail includes a set nine bilaterally symmetrical pairs sense organs known as rays. Each ray comprises three cells, which are generated by stereotyped cell sublineage expressed 18 epidermal precursor cells. A pattern formation mechanism in epidermis guides specification morphogenetic differences between rays necessary for correct organelle assembly at specific positions within epidermis. Expression these dif-ferences was altered...
Recent studies have shown that two genes regulating myogenesis (MyoD and myogenin) are coexpressed with cardiac alpha-actin during early stages of skeletal muscle development. Myogenin MyoD members a family regulatory proteins which share helix-loop-helix (HLH) motif required for dimerization DNA binding. form heterodimers the ubiquitous HLH protein E12 bind cis-acting elements an E box (CANNTG) at their core. boxes present in control regions numerous muscle-specific genes, although...
Mutations of the Pick1 gene cause globozoospermia, a male infertility disorder in both mice and human. PICK1 is critical for vesicle trafficking its deficiency sperm cells leads to abnormal from Golgi acrosome. This eventually disrupts acrosome formation infertility. We identified novel BAR-domain binding partner PICK1: ICA1L, which has sequence similarities ICA69. ICA1L expressed testes brain, major testes. are highly spermatids trafficked together at different stages spermiogenesis....
Like mechanical load in vivo, basic fibroblast growth factor (bFGF) selectively provokes cardiac expression of fetal genes including skeletal alpha-actin (SkA). Antithetically, acidic FGF (aFGF) suppresses SkA transcription. To define sites controlling transcription muscle cells, rat myocytes were transfected with internal-deletion and block-substitution mutations the promoter, three motifs resembling fos serum response element (SRE). The upstream, central, proximal SREs each contributed to...
Coordination of cell division timing is crucial for proper fate specification and tissue growth. However, the differential regulation across or within types during metazoan development remains poorly understood. To elucidate systems-level genetic architecture coordinating timing, we performed a high-content screening genes whose depletion produced significant reduction in asynchrony between sister cells (ADS) compared to that wild-type Caenorhabditis elegans embryogenesis. We quantified...
Sensory ray morphogenesis in C. elegans requires active cellular interaction regulated by multiple genetic activities. We report here the cloning of one these genes, dpy-11, which encodes a membrane-associated thioredoxin-like protein. The DPY-11 protein is made exclusively hypodermis and resides cytoplasmic compartment. Whereas TRX domain displays catalytic activity vitro, mapping lesions different mutant alleles functional analysis deletion transgenes reveal that both this enzymatic...
Embryo cryopreservation is an indispensable technique in reproductive programs and animal facilities where genetically modified mice are used extensively. Here we report the use of a vitrification spatula (VS) that can be readily homemade has large holding capacity to vitrify preimplantation mammalian embryos micro-drop employing ultra-rapid cooling liquid nitrogen (LN2). Vitrified one-cell morulae have high survival rates after thawing, fertility derived progeny comparable control...
What is the transcriptome signature associated with poor performance of rescue IVM (rIVM) oocytes and how can we rejuvenate them?The GATA-1/CREB1/WNT signalling axis was repressed in rIVM oocytes, particularly those quality; restoration this may produce more usable oocytes.rIVM aims to mature (MII) for IVF through immature collected from stimulated ovaries. It not popular due limited success rate infertility treatment. Genetic aberrations, cellular stress absence cumulus cell support could...