A5012260568- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- COVID-19 Clinical Research Studies
- RNA and protein synthesis mechanisms
- NF-κB Signaling Pathways
- Long-Term Effects of COVID-19
- Inflammatory mediators and NSAID effects
- Genetic factors in colorectal cancer
- Gene expression and cancer classification
- Single-cell and spatial transcriptomics
- Bioinformatics and Genomic Networks
- SARS-CoV-2 and COVID-19 Research
- Gene Regulatory Network Analysis
- Plant Pathogens and Fungal Diseases
- Protein Degradation and Inhibitors
- RNA regulation and disease
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- TGF-β signaling in diseases
- Genomics, phytochemicals, and oxidative stress
Nanjing Medical University
2015-2025
The University of Texas Health Science Center at Houston
2018-2022
Center for Global Health
2020
Nanjing University
2020
Nanjing General Hospital of Nanjing Military Command
2020
Hong Kong Baptist University
2013-2017
Zhejiang University
2007-2015
Nanjing University of Chinese Medicine
2014
University of Hong Kong
2014
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2013
SOX2 is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. appears to re-activate several human cancers including glioblastoma multiforme (GBM), however, detailed response program GBM has not yet been defined.We show that knockdown LN229 cells reduces cell proliferation colony formation. We then comprehensively characterize by an integrated analysis using advanced genomic technologies ChIP-seq, microarray profiling, microRNA sequencing. Using ChIP-seq...
Cavendish, the most widely grown banana cultivar, is relatively resistant to Race 1 of Fusarium oxysporum f. sp. cubense (Foc1) which caused widespread Panama disease during first half 20th century but susceptible Tropical 4 Foc (Foc TR4) threatening world production. The genome diploid species Musa acuminata ancestor a majority triploid cultivars has recently been sequenced. Availability transcriptomes will be highly useful for improving annotation and biological research. knowledge global...
Cassava is one of the most drought-tolerant crops, however, underlying mechanism for its ability to survive and produce under drought remains obscure. In this study, two cassava cultivars, SC124 Arg7, were treated by gradually reducing soil water content. Their responses stress examined through their morphological physiological traits isobaric tags relative absolute quantitation (iTRAQ)-based proteomic analysis. plants adapted a 'survival' mode mild as evidenced early stomatal closure...
Abstract Background Non small cell lung cancer (NSCLC) is one of the most common cancers in world. DHA known to be capable suppressing NSCLC proliferation and metastasis. However, mechanisms by which exhibits its antitumor effects are unknown. Here we aimed identify metabolites on growth invasion. Methods As measures invasion ability, viability transwell assays were used vitro. Transgenic mfat-1 mice, convert ω-6 PUFAs ω-3 PUFAs, detect effect endogenous tumor transplantation. An LC − MS/MS...
Abstract Transposable elements (TEs) comprise a large proportion of long non-coding RNAs (lncRNAs). Here, we employed CRISPR to delete short interspersed nuclear element (SINE) in Malat1, cancer-associated lncRNA, investigate its significance cellular physiology. We show that Malat1 with SINE deletion forms diffuse speckles and is frequently translocated the cytoplasm. SINE-deleted cells exhibit an activated unfolded protein response PKR markedly increased DNA damage apoptosis caused by...
Systematic characterization of ẖybrid incompatibility (HI) between related species remains the key to understanding speciation. The genetic basis HI has been intensively studied in Drosophila species, but largely unknown other including nematodes, which is mainly due lack a sister with C. elegans can mate and produce viable progeny. recent discovery briggsae nigoni, opened up possibility dissecting nematode species. However, paucity dominant visible marker prevents efficient mapping loci two...
Cancer-associated fibroblasts (CAFs) are critically involved in tumor progression by maintaining extracellular mesenchyma (ECM) production and improving development. Cyclooxygenase-2 (COX-2) has been proved to promote ECM formation progression. However, the mechanisms of COX-2 mediated CAFs activation have not yet elucidated. Therefore, we conducted this study identify effects underlying tumor-derived exosomal miRNAs lung adenocarcinoma (LUAD) progression.As measures activation, expressions...
SOX2 is an HMG box containing transcription factor that has been implicated in various types of cancer, but its role colorectal cancers (CRC) not studied. Here we show overexpressed CRC tissues compared with normal adjacent using immunohistochemical staining and RT-PCR. We also observed increased expression nucleus cancer (46%, 14/30 cases vs. 7%, 2/30 tissues). Furthermore, knockdown SW620 cells decreased their growth rates vitro cell line, vivo xenograft models. ChIP-Seq analysis revealed...
Cyclooxygenase-2 (COX-2) has been implicated in cell invasion non-small-cell lung cancer (NSCLC). However, the mechanism is unclear. The present study investigated effect of COX-2 on β1-integrin expression and NSCLC. were co-expressed NSCLC tissues. overexpression or Prostaglandin E2 (PGE2) treatment increased lines. silencing suppressed COX-2-mediated tumour growth vivo vitro. E Receptor EP1 transfection with agonist mimicked PGE2 treatment. siRNA blocked PGE2-mediated expression. promoted...
Chemoresistance poses a significant challenge in colorectal cancer (CRC) treatment. However, the mechanisms underlying chemoresistance remain unclear. CBX3 promoted proliferation and metastasis CRC. role mechanism of unknown. Therefore, we aimed to investigate effects on multidrug resistance Our studies showed that higher levels expression were associated with poor survival, especially groups progression following chemotherapy. overexpression increased Irinotecan Oxaliplatin resistance,...
SOX4 is a transcription factor required for tissue development and differentiation in vertebrates. Overexpression of has been reported many cancers including glioblastoma multiforme (GBM), however, the underlying mechanism actions not studied. In this study, we investigated role GBM. Kaplan-Meier analysis was performed to assess association between expression levels survival times primary GBM samples. Cre/lox P system used generate gain or loss cells, microarray uncovered regulation network...
In head and neck squamous cell carcinoma (HNSCC), mutations of p53 usually coexist with aberrant activation NF-kappaB (NF-κB), other transcription factors microRNAs, which promote tumor pathogenesis. However, how these microRNAs interact to globally modulate gene expression mediate oncogenesis is not fully understood. We devised a novel bioinformatics method uncover interactive relationships between or genes. This approach based on matrix decomposition modeling under the joint constraints...
Coordination of cell division timing is crucial for proper fate specification and tissue growth. However, the differential regulation across or within types during metazoan development remains poorly understood. To elucidate systems-level genetic architecture coordinating timing, we performed a high-content screening genes whose depletion produced significant reduction in asynchrony between sister cells (ADS) compared to that wild-type Caenorhabditis elegans embryogenesis. We quantified...
The nuclear pore complex (NPC) comprises more than 30 nucleoporins (NUPs) and is a hallmark of eukaryotes. NUPs have been suggested to be important in regulating gene transcription 3D genome organization. However, evidence support their direct roles remains limited. Here, by Cut&Run, we find that core display broad but also cell-type-specific association with active promoters enhancers human cells. Auxin-mediated rapid depletion two demonstrates NUP93, not NUP35, directly specifically...
Abstract Summary: ChIP-seq technology provides an accurate characterization of transcription or epigenetic factors binding on genomic sequences. With integration such ChIP-based and other high-throughput information, it would be dedicated to dissecting cross-interactions among multilevel regulators, genes biological functions. Here, we devised integrative web server CMGRN (constructing gene regulatory networks), unravel hierarchical interactive networks at different levels. The newly...
Interactions among transcriptional factors (TFs), cofactors and other proteins or enzymes can affect regulatory capabilities of eukaryotic organisms.Post-translational modifications (PTMs) cooperate with TFs epigenetic alterations to constitute a hierarchical complexity in gene regulation.While clearly implicated biological processes, our understanding these complex mechanisms is still limited incomplete.Various online software have been proposed for uncovering networks, however, there lack...
Colorectal cancer (CRC) is a heterogeneous disease that associated with gradual accumulation of genetic and epigenetic alterations. Among all CRC stages, stage II tumors are highly high relapse rate in about 20–25 % patients following surgery. Thus, comprehensive analysis gene signatures to identify aggressive metastatic phenotypes desired for more accurate classification outcome prediction. By utilizing Cancer Array, containing 440 oncogenes tumor suppressors profile mRNA expression, we...
Cell fate specification is typically initiated by a master regulator, which relayed tissue-specific regulatory proteins (usually transcription factors) for further enforcement of cell identities, but how the factors are coordinated among each other to "finish up" remains poorly understood. <i>Caenorhabditis elegans</i> epidermis ELT-1, that activates its targets, NHR-25 and ELT-3, two epidermis-specific important development not initial epidermis, thus providing unique paradigm illustrating...
Infection with SARS-CoV-2 has been associated liver dysfunction, aggravation of burden, and injury. This study aimed to assess the effects injuries on clinical outcomes patients COVID-19. A total 1520 severe or critical COVID-19 from Huoshenshan Hospital, Wuhan, were enrolled. Chronic disease (CLD) was confirmed by consensus diagnostic criteria. Laboratory test results compared between different groups. scRNA-seq data bulk gene expression profiles used identify cell types 10.98% developed...
Due to the increased morbidity, mortality, and cost of community-acquired pneumonia (CAP) in older people, strategies directed at improving disease evaluation prevention are imperative. We independently compared 30-day in-hospital mortality prediction ability a frailty index based on laboratory data (FI-Lab) with that CURB-65 Pneumonia Severity Index (PSI) then proposed combining them further improve efficiency.
Alternative polyadenylation (APA) is widely present in the human genome and plays a key role carcinogenesis. We conducted comprehensive analysis of APA products glioblastoma multiforme (GBM, one most lethal brain tumors) normal tissues further developed computational pipeline, RNAelements (http://sysbio.zju.edu.cn/RNAelements/), using covariance model from known RNA binding protein (RBP) targets acquired by Immunoprecipitation (RIP) analysis. identified 4530 isoforms for 2733 genes GBM,...