A5100705616- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Mathematical Biology Tumor Growth
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Bioinformatics and Genomic Networks
- COVID-19 Clinical Research Studies
- Immunodeficiency and Autoimmune Disorders
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Cancer Immunotherapy and Biomarkers
- Cell death mechanisms and regulation
- Cancer-related Molecular Pathways
- Immune Cell Function and Interaction
- Viral-associated cancers and disorders
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Wnt/β-catenin signaling in development and cancer
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Chronic Myeloid Leukemia Treatments
- CAR-T cell therapy research
- MicroRNA in disease regulation
- RNA Research and Splicing
- Glioma Diagnosis and Treatment
Nanjing Medical University
2014-2024
Jiangsu Province Hospital
2013-2023
Jiangsu Cancer Hospital
2022-2023
Zhejiang University
2022-2023
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2022-2023
National Clinical Research
2023
Southeast University
2021-2022
First Affiliated Hospital of Guangzhou University of Chinese Medicine
2021
Wuyi University
2021
Università della Svizzera italiana
2020
Deciphering the dynamic changes in antibodies against SARS-CoV-2 is essential for understanding immune response COVID-19 patients. Here we analyze laboratory findings of 1,850 patients to describe total antibody, spike protein (S)-, receptor-binding domain (RBD)-, and nucleoprotein (N)-specific immunoglobulin M (IgM) G (IgG) levels during infection recovery. The generation S-, RBD-, N-specific IgG occurs one week later with severe/critical compared mild/moderate disease, while S-...
Abstract Background Males and females differ in their immunological responses to foreign pathogens. However, most of the current COVID-19 clinical practices trials do not take sex factor into consideration. Methods We performed a sex-based comparative analysis for outcomes, peripheral immune cells, severe acute respiratory syndrome coronavirus (SARS-CoV-2) specific antibody levels 1558 males 1499 patients from single center. The lymphocyte subgroups were measured by Flow cytometry. total...
Abstract Isocitrate dehydrogenase (IDH) wild-type glioblastoma (GBM) has a dismal prognosis. A better understanding of tumor evolution holds the key to developing more effective treatment. Here we study GBM's natural evolutionary trajectory by using rare multifocal samples. We sequenced 61,062 single cells from eight IDH primary GBMs and defined signature (NES) tumor. show that NES significantly associates with activation transcription factors regulate brain development, including MYBL2...
Abstract Background Chloroplast genome resources can provide useful information for the evolution of plant species. Tea ( Camellia sinensis ) is among most economically valuable member . Here, we determined chloroplast first natural triploid Chinary type tea (‘Wuyi narcissus’ cultivar var. , CWN and conducted comparison with diploid CSS two types Assamica teas assamica : Chinese tea, CSA Indian CIA ). Further, evolutionary mechanism relationships species based on were discussed. Results...
Abstract Chemoresistance and relapse are the leading cause of AML-related deaths. Utilizing single-cell RNA sequencing (scRNA-seq), we dissected cellular states bone marrow samples from primary refractory or short-term relapsed AML patients defined transcriptional intratumoral heterogeneity. We found that compared to proliferating stem/progenitor-like cells (PSPs), a subpopulation quiescent stem-like (QSCs) were involved in chemoresistance poor outcomes AML. By performing longitudinal...
Proneural (PN) to mesenchymal (MES) transition (PMT) is a crucial phenotypic shift in glioblastoma stem cells (GSCs). However, the mechanisms driving this process remain poorly understood. Here, we report that Fos-like antigen 1 (FOSL1), component of AP1 transcription factor complexes, key player regulating PMT. FOSL1 predominantly expressed MES subtype, but not PN GSCs. Knocking down expression GSCs leads loss features and tumor-initiating ability, whereas ectopic able induce PMT maintain...
Male breast cancer (MBC) is a rare but aggressive malignancy with cellular and immunological characteristics that remain unclear. Here, we perform transcriptomic analysis for 111,038 single cells from tumor tissues of six MBC thirteen female (FBC) patients. We find has significantly lower infiltration T relative to FBC. Metastasis-related programs are more active in MBC. The activated fatty acid metabolism involved FASN related cell metastasis low immune show activation p38 MAPK lipid...
Abstract Tumor-associated macrophages (TAM) play a crucial role in immunosuppression. However, how TAMs are transformed into immunosuppressive phenotypes and influence the tumor microenvironment (TME) is not fully understood. Here, we utilized single-cell RNA sequencing whole-exome data of glioblastoma (GBM) tissues identified subset dually expressing macrophage signatures, which were termed double-positive TAMs. Double-positive tended to be bone marrow–derived (BMDM) characterized by...
MicroRNA (miRNA, miR)-18a is a member of the miR-17–92 cluster, an important locus that markedly overexpressed in several cancers and associated with cancer development progression. However, mechanism action cluster its individual miRNAs are largely unknown. In this study, we investigated expression by situ hybridisation (ISH) assay copy-number analysis gastric tissue microarray (TMA) specimens. We determined miR-18a was present at higher levels than other five cluster. addition, identified...
Circular RNAs (circRNAs) have recently been reported to play crucial roles in various regulatory processes and involved cancer onset progression. However, the potential mechanism of circRNAs chronic lymphocytic leukemia (CLL) remains largely unknown. Here, we observed hsa_circ_0132266 (circ_0132266), a circRNA significantly decreased peripheral blood mononuclear cells (PBMCs) CLL patients compared with healthy donors, could act as an endogenous sponge hsa-miR-337-3p (miR-337-3p) regulate its...
// Yi Xia 1 , Lei Fan Li Wang Robert Peter Gale 2 Man Tian Wei Wu Liang Yu 3 Yao-Yu Chen Xu and Jian-Yong Department of Hematology, The First Affiliated Hospital Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China Haematology Research Centre, Division Experimental Medicine, Imperial College London, United Kingdom Huai'an People's Huai'an, Province, Correspondence: Xu, email: Li, Keywords : SF3B1, NOTCH1, MYD88, mutation, chronic lymphocytic leukemia Received September 01,...
Hemophagocytic lymphohistiocytosis (HLH) can be classified into primary HLH and secondary HLH. Primary usually occurs in infants children with an underlying genetic defect, there are also teens occasional adults Most cases adult patients triggers including infections, malignancies, autoimmune diseases. The distinction between seems to less straightforward, as may have defects while triggered by causes. In this study, using amplicon-based targeted gene sequencing (TGS), we sequenced eighteen...
Aberrant activation of lymphoid enhancer-binding factor-1 (LEF1) has been identified in several cancers, including chronic lymphocytic leukemia (CLL). As a key transcription factor the Wnt/β-catenin pathway, LEF1 helps to regulate important genes involved tumor cell death mechanisms. In this study, we determined gene expression levels CLL (n = 197) and monoclonal B-cell lymphocytosis (MBL) 6) patients through real-time RT-PCR. was significantly up-regulated both MBL compared with normal B...
The role of respiratory tract microbes and the relationship between gut microbiomes in coronavirus disease 2019 (COVID-19) remain uncertain. Here, metagenomes sputum fecal samples from 66 patients with COVID-19 at three stages progression are sequenced. Respiratory tract, microbiome, peripheral blood mononuclear cell (PBMC) analyzed to compare microbiota intensive care unit (ICU) non-ICU (nICU) determine relationships microbiome immune response. In significantly fewer Streptococcus,...
Loss of cylindromatosis gene (CYLD) expression has been observed in various cancers, including chronic lymphocytic leukemia (CLL). As a deubiquitination enzyme, CYLD regulates the proliferation, development and activation lymphoid cells. Here we determined mRNA by quantitative polymerase chain reaction (PCR) 125 patients with CLL. was considerably down-regulated CLL cells compared to normal B Low associated unmutated status immunoglobulin heavy-chain variable-region (IGHV) (p = 0.0018) CD38...
Lymphoid enhancer-binding factor 1 (LEF1) is a downstream effector of the Wnt/β-catenin signaling pathway and its dysregulation associated with number malignant diseases such as leukemia. We explored expression profile LEF1 in acute myeloid leukemia (AML) determined specific prognostic significance this disease. The mRNA level patients previously untreated AML was significantly higher than normal controls. Patients relatively were more likely to achieve complete remission (CR) following...
Abstract Background COVID-19 has resulted in high mortality worldwide. Information regarding cardiac markers for precise risk-stratification is limited. We aim to discover sensitive and reliable early-warning biomarkers optimizing management improving the prognosis of patients. Methods A total 2954 consecutive patients who were receiving treatment from Wuhan Huoshenshan Hospital China February 4 April 10 included this retrospective cohort. Serum levels collected after admission. Coronary...
Coronavirus disease 2019 (COVID-19) has rapidly spread worldwide. Systematic analysis of lung cancer survivors at molecular and clinical levels is warranted to understand the course characteristics.A single-center, retrospective cohort study was conducted in 65 patients with COVID-19 from Wuhan Huoshenshan Hospital, which 13 were diagnosed cancer. The February 4 April 11, 2020.During treatment, infected severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) had shorter median time...
Chronic lymphocytic leukemia (CLL) is one of the most frequent type B-cell chronic lymphoproliferative disorders and inflammation takes part in development CLL. However, there has been no valid immune biomarker to predict prognosis untreated CLL patients.In this retrospective study, we analyzed clinical correlations prognostic value albumin-to-fibrinogen ratio (AFR) detected at diagnosis 191 patients.The cut-off AFR was 9.7 calculated by X-tile. Patients who were more than 65 years old often...