A5011666777- Cancer-related molecular mechanisms research
- Immune cells in cancer
- Hepatitis B Virus Studies
- Bone and Joint Diseases
- Hepatitis Viruses Studies and Epidemiology
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Hepatitis C virus research
- Cytokine Signaling Pathways and Interactions
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Pneumonia and Respiratory Infections
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Glycosylation and Glycoproteins Research
- Hip disorders and treatments
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Lipids, and Metabolism
- RNA Research and Splicing
- Circular RNAs in diseases
- Liver Disease Diagnosis and Treatment
- Extracellular vesicles in disease
- Autophagy in Disease and Therapy
- Cancer Immunotherapy and Biomarkers
Chongqing Medical University
2016-2025
Children's Hospital of Chongqing Medical University
2014-2024
Zhenjiang City Fourth People's Hospital
2016-2022
Jiangsu University
2016-2022
Affiliated Hospital of Jiangsu University
2021
Sun Yat-sen University
2020
Chongqing Maternal and Child Health Hospital
2019
HZ University of Applied Sciences
2015
Nanjing Medical University
2014-2015
Jiangsu Province Hospital
2015
Progranulin (PGRN), as a multifunctional growth factor, is overexpressed in multiple tumors, but the role of PGRN on tumor immunity still unclear. Here, we studied effect breast cancer and its possible molecular mechanism.The changes macrophage phenotypes after treatment were detected by western blot, quantitative polymerase chain reaction (PCR) flow cytometry. Western blot was used to study signal mechanism regulating this process. The number localization immune cells Wild-type (WT) PGRN-/-...
Tumor-associated macrophages (TAMs) are essential cellular components within tumor microenvironment (TME). TAMs educated by TME to transform M2 polarized population, showing a M2-like phenotype, IL-10high, IL-12low, TGF-βhigh. STAT3 signaling triggers crosstalk between cells and TAMs, is crucial for the regulation of malignant progression. In our study, legumain-targeting liposomal nanoparticles (NPs) encapsulating HC were employed suppress activity "re-educate" investigate effects...
Background Information: Previous studies have revealed that leptin may be involved in epithelial-mesenchymal transition (EMT), a crucial initiator of cancer progression to facilitate metastatic cascade, increase tumor recurrence, and ultimately cause poor prognosis. However, the underlying mechanism remains unclear. The aim our present study was investigate effect on EMT breast cells mechanism. Results: Our data demonstrated significantly increased phosphorylation STAT3, Akt, ERK1/2,...
In recent years, crosstalk between tumor microenvironment and cancer cells have received increasing attention. Accumulating research data suggests that leptin, a key adipokine secreted from adipocytes, plays important roles in breast development. our study, the effects of leptin on polarization tumor-associated macrophages (TAMs) promotion invasiveness were investigated. THP1 used to differentiate M2 macrophages. After stimulated by we established co-culture system evaluate function...
Accumulating researches have shown that epithelial-mesenchymal transition (EMT) contributes to tumor metastasis. Leptin, a key adipokine secreted from adipocytes, shapes the microenvironment, potentiates migration of breast cancer cells and angiogenesis, is also involved in EMT. However, potential mechanism remains unknown. This study aims explore effect leptin on EMT underlying mechanism. With assessment EMT-associated marker expression MCF-7, SK-BR-3, MDA-MB-468 cells, was analyzed....
Circulating tumor cells (CTCs) are the seeds of distant metastases malignant tumors and associated with malignancy risk metastasis. However, undergo epithelial-mesenchymal transition (EMT) during metastasis, leading to emergence different types CTCs. Real-time dynamic molecular functional typing CTCs is necessary precisely guide personalized treatment. Most CTC detection systems based on epithelial markers that may fail detect EMT Therefore, it clinically important identify new types. In...
The tumor microenvironment (TME) mediates immunosuppression resulting in cell escape from immune surveillance and cancer vaccine failure. Immunosuppression is mediated by the STAT-3 transcription factor, which potentiates signaling cells. Because continues to be a major inhibitor of efficacy, we examined this study whether therapeutically targeted delivery synthetic TME, combined with an HER-2 DNA can improve against HER-2(+) breast prevent its recurrence. To end, developed novel...
This study aims to investigate the mechanisms underlying leptin-mediated crosstalk between tumor-associated macrophages (M2 macrophages) and breast cancer cells. THP1 human leukemic monocytes were induced differentiate into M2 by PMA (100 nM) IL-4 (20 ng/mL). Quantitative RT-PCR Western blot revealed that leptin significantly increased expression of receptor (ObR) in (P < 0.01) stimulated interleukin (IL)-8 macrophages, mouse macrophage cells RAW264.7, primary peritoneal a dose-...
Abstract Background Nontraumatic osteonecrosis of the femoral head (NONFH) is a common, progressive, and refractory orthopaedic disease. Decreased osteogenesis angiogenesis are considered main factors in pathogenesis NONFH. We aimed to figure out whether exosomes exosomal miRNA from necrotic bone tissues patients with NONFH involved reveal underlying mechanisms. Methods RT-PCR western blotting (WB) were used detect expression osteogenic, adipogenic, angiogenic markers. ALP staining Alizarin...
As CRISPR technology is promoted to more fine-divided molecular biology applications, its inherent performance finds it increasingly difficult cope with diverse needs in these different fields, and how accurately control the has become a key issue develop new stage. Herein, we propose CRISPR/Cas12a regulation strategy based on powerful programmability of nucleic acid nanotechnology. Unlike previous rigid core components Cas nuclease crRNA, only simple switch external RNA accessories required...
Abstract The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system exhibits extraordinary capability in the field of molecular diagnosis and biosensing, attributed to its trans-cleavage ability. precise modulation performance has emerged as a significant challenge advancing CRISPR technology next stage development. Herein, we reported CRISPR/Cas12a regulation strategy based on an overhanging activator. presence domains activators creates steric hindrances that have...
Metastasis is a major cause of prostate cancer (PCa)-related deaths in men. Recent studies have indicated that VGF nerve growth factor inducible (VGF) affects tumor invasion and metastasis. The present study investigated whether abnormally expressed PCa progression the specific regulatory mechanisms by which sh- hypoxia-inducible factor1 alpha (HIF-1α) plasmid was transfected into human cell lines 22Rv1 C4-2 to create with stable low expression overexpression VGF. Quantitative PCR (qPCR)...
Progression to castration resistance is the leading cause of death in prostate cancer patients. Long non-coding RNAs (lncRNAs) have recently become a focal point regulation development. However, few lncRNAs associated with castration-resistant (CRPC) been reported. Firstly, we explore CRPC by RNA sequencing and validated using quantitative polymerase chain reaction (qRT-PCR) fluorescence situ hybridization (RNA-FISH). The clinical significance FLJ was evaluated collected cohort. Functional...
Abstract Non-traumatic osteonecrosis of the femoral head (ONFH) is clinically a devastating and progressive disease without an effective treatment. Mesenchymal stem cells (MSCs) transplantation has been used to treat ONFH in early stage, but failure rate this therapy high due reduced osteogenic differentiation migration transplanted MSCs related with pathological bone tissues. However, mechanism responsible for decrease still unclear. Therefore, we assume that implanted might be influenced...