A5033131707- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Electrochemical sensors and biosensors
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Advanced Nanomaterials in Catalysis
- Electrochemical Analysis and Applications
- CRISPR and Genetic Engineering
- Molecular Junctions and Nanostructures
- RNA modifications and cancer
- Analytical Chemistry and Sensors
- Advanced Biosensing Techniques and Applications
- Fire Detection and Safety Systems
- Nanopore and Nanochannel Transport Studies
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Nanoplatforms for cancer theranostics
- Video Surveillance and Tracking Methods
- Knee injuries and reconstruction techniques
- Wnt/β-catenin signaling in development and cancer
- Biomedical Text Mining and Ontologies
- Click Chemistry and Applications
Children's Hospital of Chongqing Medical University
2016-2025
Ningbo University
2013-2025
Chongqing Medical University
2016-2025
Shanghai Sixth People's Hospital
2011-2024
Shanghai Jiao Tong University
2019-2024
Ningbo Medical Center Lihuili Hospital
2022-2024
Chongqing University
2013-2022
Xi'an University of Technology
2020-2022
Ministry of Education
2017-2021
Second Military Medical University
2015
New agents with particular specificity toward targeted bacteria and superefficacy in antibacterial activity are urgently needed facing the crisis of worldwide antibiotic resistance. Herein, a novel strategy by equipping bacteriophage (PAP) photodynamic inactivation (PDI)-active AIEgens (luminogens aggregation-induced emission property) was presented to generate type AIE–PAP bioconjugate superior capability for both imaging synergistic killing certain species bacteria. The targeting ability...
Efficient signal amplification is essential to construct ultrasensitive biosensors for biologically relevant species with abundant concomitant interferences. Here, we apply LbaCas12a as a amplifier develop versatile CRISPR-Cas12a platform detect wide range of analytes in ultralow concentrations. The relies on the indiscriminate single-stranded DNase activity LbaCas12a, which recognizes DNA intermediates generated by non-DNA targets down femtomolar concentrations and subsequently enhances...
CRISPR (clustered regularly interspaced short palindromic repeats) technology has achieved great breakthroughs in terms of convenience and sensitivity; it is becoming the most promising molecular tool. However, only two activation modes (single double stranded) are available, they have specificity universality bottlenecks that limit application high-precision recognition. Herein, we proposed a novel CRISPR/Cas12a unrestricted mode to greatly improve its performance. The new totally...
Abstract Although gene expression signatures offer tremendous potential in diseases diagnostic and prognostic, but massive caused challenges for experimental detection computational analysis clinical setting. Here, we introduce a universal DNA-based molecular classifier profiling generating immediate outcomes. The begins with feature transformation, modular programmable strategy was used to capture relative relationships of low-concentration RNAs convert them general coding inputs. Then,...
The accurate and early detection of Mycobacterium tuberculosis (Mtb) is great significance for the clinical diagnosis treatment tuberculosis. In this work, we report a facile method controllable synthesis novel few-layered two-dimensional graphdiyne nanosheet (GDY NS) with thickness only ∼0.9 nm via an electrochemical lithium-intercalation strategy, which possesses prominent fluorescence quenching effect. GDY NS its strong adsorptivity single-stranded DNA first proposed as new fluorescent...
As detection signal, UCNP fluorescence could improve the ability of biosensor in complex biological samples. UCNPs unbound to bacteria be separated by magnet, improving sensitivity and time biosensor.
As CRISPR technology is promoted to more fine-divided molecular biology applications, its inherent performance finds it increasingly difficult cope with diverse needs in these different fields, and how accurately control the has become a key issue develop new stage. Herein, we propose CRISPR/Cas12a regulation strategy based on powerful programmability of nucleic acid nanotechnology. Unlike previous rigid core components Cas nuclease crRNA, only simple switch external RNA accessories required...