A5100599307- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- MicroRNA in disease regulation
- Geological and Geochemical Analysis
- Molecular Biology Techniques and Applications
- High-pressure geophysics and materials
- Innovative Microfluidic and Catalytic Techniques Innovation
- Chemical Synthesis and Analysis
- Bacteriophages and microbial interactions
- earthquake and tectonic studies
- Folate and B Vitamins Research
- Advanced Biosensing Techniques and Applications
- Geochemistry and Geologic Mapping
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- Burkholderia infections and melioidosis
- Cancer Research and Treatments
- Sulfur-Based Synthesis Techniques
- Insect symbiosis and bacterial influences
- Receptor Mechanisms and Signaling
- Conducting polymers and applications
- Bacterial Identification and Susceptibility Testing
- Advanced Synthetic Organic Chemistry
Hainan Medical University
2023-2025
Chongqing Medical University
2014-2023
Affiliated Hospital of Southwest Medical University
2017-2022
Children's Hospital of Chongqing Medical University
2015-2022
China Geological Survey
2013-2022
Ministry of Natural Resources
2021
Chongqing University
2016
The University of Texas Southwestern Medical Center
2014-2016
Southwestern Medical Center
2016
University of Science and Technology of China
2014
Multiplex one-step direct PCR of blood for SNP genotyping.
DNA strand displacement plays an essential role in the field of dynamic nanotechnology. However, flexible regulation remains a significant challenge. Most previous regulatory tools focused on controllable activation toehold and thus limited design flexibility. Here, we introduce tool termed cooperative branch migration (CBM), through which can be controlled by regulating complementarity domains. CBM shows perfect compatibility with majority existing tools, when combined forked toehold, it...
Loop-mediated isothermal amplification (LAMP) is a commonly used alternative to PCR for point-of-care detection of nucleic acids due its rapidity, sensitivity, specificity, and simpler instrumentation. While dual-labeled TaqMan probes are widely in single-nucleotide polymorphism (SNP) genotyping, real-time LAMP primarily relies on turbidimetry or intercalator fluorescence measurements, which can be non-specific generate false-positive results. In this study, we propose closed-tube,...
An isothermal, enzyme free, ultra-specific and ultra-sensitive protocol for electrochemical detection of miRNAs is proposed based on the toehold-mediated strand displacement reaction (SDR) non-enzymatic catalytic hairpin (CHA) recycling. The SDR was first triggered only in presence target miRNA this process also affects other interferences having similar sequences, thus guaranteeing a high discrimination factor could be used rare content with various amounts sequences. output protector then...
A novel method for the synthesis of polysubstituted tetrahydro-4H-pyrido[1,2-a]pyrimidines through a domino desulfurative coupling-acylation-hydration and Michael addition sequence was established.
Genotyping of folate metabolism genes is great importance in disease diagnosis and prevention. However, most current detection methods used for gene genotyping are based on sequencing chips, which suffer from a high cost laborious time-consuming procedures. Herein, we reported multiplex asymmetric PCR-HRM strategy identifying genotypes single tube. The proposed assay has been successfully applied to identify the genes, methylene tetrahydrofolate reductase (C677T, A1298C) methionine synthase...
Detection of single nucleotide polymorphisms (SNPs) is critical for personalized clinical diagnosis, treatment, and medication. Current detection methods suffer from primer dimerization require the redesigning reaction systems different targets, resulting in a time-consuming laborious process. Here, we present robust versatile method SNP typing by using tailed primers universal small molecule probes combination with visualized lateral flow assay (LFA). This approach enables not only rapid...
In this article, we reported a double-stranded DNA probe modified gold nanoparticle used as both “nano-flares” and transfection agents to quantify miR-30a in solution visualize live cells.