A5100410073- Bone and Joint Diseases
- Hip disorders and treatments
- Osteoarthritis Treatment and Mechanisms
- Bone health and treatments
- Cancer-related molecular mechanisms research
- Total Knee Arthroplasty Outcomes
- Extracellular vesicles in disease
- Knee injuries and reconstruction techniques
- Mesenchymal stem cell research
- Lower Extremity Biomechanics and Pathologies
- Peroxisome Proliferator-Activated Receptors
- Bone fractures and treatments
- Periodontal Regeneration and Treatments
- Diabetic Foot Ulcer Assessment and Management
- Bone Tissue Engineering Materials
- Retinoids in leukemia and cellular processes
- Orthopedic Infections and Treatments
- Orthopaedic implants and arthroplasty
- Metabolomics and Mass Spectrometry Studies
- Pregnancy and Medication Impact
- Healthcare and Venom Research
- Autoimmune and Inflammatory Disorders Research
- Acute Myeloid Leukemia Research
- Barrier Structure and Function Studies
- Bone and Dental Protein Studies
Chongqing Medical University
2016-2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2016-2024
Dalian Medical University
2023
First Affiliated Hospital of Dalian Medical University
2023
Second Affiliated Hospital of Nanchang University
2023
Nanchang University
2023
Harbin Medical University
2021-2023
Second Affiliated Hospital of Harbin Medical University
2021-2023
Shandong Normal University
2023
PLA Academy of Military Science
2022
Survival and stemness of bone marrow mesenchymal stem cells (BMSCs) in osteonecrotic areas are especially important the treatment early steroid-induced osteonecrosis femoral head (ONFH). We had previously used BMSCs to repair ONFH, but transplanted underwent a great deal stress-induced apoptosis aging oxidative-stress (OS) microenvironment femoral-head necrotic area, which limited their efficacy. Our subsequent studies have shown that under OS, massive accumulation damaged mitochondria is an...
Abstract Novel therapies for the treatment of early steroid-induced osteonecrosis femoral head (SONFH) are urgently needed in orthopedics. Transplantation bone marrow mesenchymal stem cells (BMSCs) provides new strategies treating this condition at stage. However, stress-induced apoptosis BMSCs transplanted into necrotic area limits efficacy BMSC transplantation. Inhibiting is key to improving procedure. In our previous studies, we confirmed that Parkinson disease protein 7 (PARK7) active...
Abstract Background Nontraumatic osteonecrosis of the femoral head (NONFH) is a common, progressive, and refractory orthopaedic disease. Decreased osteogenesis angiogenesis are considered main factors in pathogenesis NONFH. We aimed to figure out whether exosomes exosomal miRNA from necrotic bone tissues patients with NONFH involved reveal underlying mechanisms. Methods RT-PCR western blotting (WB) were used detect expression osteogenic, adipogenic, angiogenic markers. ALP staining Alizarin...
Introduction This study aimed to investigate the effect of muscle-derived stem cell (MDSC) exosomes with overexpressed miR-214 on regeneration and repair rat sciatic nerve after crush injury its molecular mechanism. Methods First, primary MDSCs, Schwann cells (SCs) dorsal root ganglion (DRG) neurons were isolated cultured, characteristics MDSCs-derived identified by biology immunohistochemistry. NC mimics transfected obtain exo-NC exo-miR-214. An in vitro co-culture system was established...
Osteophyte is one of the inevitable consequences progressive osteoarthritis with main characteristics cartilage degeneration and endochondral ossification. The pathogenesis osteophyte formation not fully understood to date. In this work, metabolomic approaches were employed explore potential mechanisms by detecting metabolic variations between extracts tissues (n = 32) uninvolved control 34), based on platform ultraperformance liquid chromatography tandem quadrupole time-of-flight mass...
Avascular necrosis of the femoral head (ANFH) is focus and difficulty orthopedic diseases. Recently, tissue engineering bone for this disease has shown a good therapeutic effect. The aim present study was to investigate effect basic fibroblast growth factor (FGF-2) as cytokines transfected marrow mesenchymal stem cells (BMSCs) in constructing tissue-engineered avascular head.The FGF-2 gene overexpressed lentivirus-transfected rBMSCs with xenogeneic antigen-extracted cancellous (XACB)...
Elevated plasma level of homocysteine, also termed as hyperhomocysteinemia, is acknowledged a significant and independent risk factor Alzheimer's disease. However, the mechanistic insight has not been thoroughly elucidated yet. In this work, 3,5-dihydroxybenzyloxy was explored unique reaction trigger integrated into naphthalimide fluorophore via carbamate linker to afford new probe for •OH imaging. treatment induced aromatic hydroxylation subsequent elimination release caged fluorophore,...
The differentiation fate of bone marrow mesenchymal stem cells (BMSCs) affects the progression steroid-induced osteonecrosis femoral head (SONFH). We find that lncRNA DGCR5 encodes a 102-amino acid polypeptide, RIP (Rac1 inactivated peptide), which promotes adipogenic BMSCs and aggravates SONFH. RIP, instead DGCR5, binds to N-terminal motif RAC1, inactivates RAC1/PAK1 cascade, resulting in decreased Ser675 phosphorylation β-catenin. Ultimately, nuclear localization β-catenin decreases,...
Intervertebral disc degeneration (IDD) is the primary cause of neck and back pain. Obesity has been established as a significant risk factor for IDD. The objective this study was to explore molecular mechanisms affecting obesity IDD by identifying overlapping crosstalk genes associated with both conditions. identification specific diagnostic biomarkers would have crucial clinical implications. We obtained gene expression profiles GSE70362 GSE152991 from Gene Expression Omnibus, followed...
Abstract The management of infected bone defects poses a significant clinical challenge, and current treatment modalities exhibit various limitations. This study focuses on the development multifunctional composite scaffold comprising nanohydroxyapatite/polyethyleneglycol diacrylate matrix, silver nanoparticles, graphene oxide (GO), sodium alginate, M2‐type macrophage membrane vesicles (MVs) to enhance healing defects. demonstrates several key features: first, it releases sufficient...
The study aims to investigate the effect of microRNA-34a (miR-34a) targeting Tgif2 on steroid-induced avascular necrosis femoral head (SANFH) by regulating OPG/RANK/RANKL signaling pathway. SD rats were divided into normal control and model (RNAKL rat models) groups. group was further assigned control, negative miR-34a mimics inhibitors QRT-PCR applied detect miR-34a, Tgif2, OPG, RANK RNAKL mRNA expressions. Femoral tissues collected for Micro-CT scanning HE staining. Western blotting used...
Immunosuppressant-related hip pain can greatly affect a patient's mobility and increase the number of total arthroplasties. We investigated risk factors causes after orthotopic liver transplant.The medical records 175 adult transplant patients, who were followed-up for more than 2 years, retrospectively reviewed. Data collected from included primary disease, medications, biochemical results, Child-Turcotte-Pugh score, death, rejection, complications related to transplant.A 11 patients (6.3%)...
Early diagnosis and treatment of osteonecrosis the femoral head (ONFH) is challenging. Bone trabecula play a vital role in severity progression ONFH. In present study, investigators used gene expression profiling bone to investigate alterations ONFH patients. Osteonecrotic (ONBT) such as necrosis, fibrosis, lacuna were confirmed by histological examination The adjacent 'normal' (ANBT) did not show any pathological changes. Gene sequencing data revealed that although ANBT showed no...
Osteonecrosis of the femoral head (ONFH) is a disorder that can cause collapse head. The damage and dysfunction microvascular endothelial cells are related to pathogenesis glucocorticoid-induced ONFH. Reports suggest vitamin B2 promote osteoblast differentiation prevent low bone mineral density reperfusion oxidative injury. To explore effect possible molecular mechanism on ONFH Human Umbilical Vein Endothelial Cells (HUVECs), we performed rat model by dexamethasone. rats were randomly...