A cascade signal amplification strategy was proposed for detection of protein target at ultralow concentration by combining the rolling circle (RCA) technique with oligonucleotide functionalized quantum dots (QDs), multiplex binding biotin−strepavidin system, and anodic stripping voltammetric detection. The RCA product containing tandem-repeat sequences could serve as excellent template periodic assembly QDs, which presented per recognition event to numerous dot tags electrochemical readout....

A target-switched DNA nanotweezer is designed for AND logic gate operation and enzyme-free detection of microRNAs (miRNAs) by catalytic hairpin assembly (CHA) proximity-dependent DNAzyme formation. The double crossover motif-based consists an arched structure as the set strand target inputs two split G-rich DNAs at termini arms signal output. Upon a CHA, small amount binary can switch numerous open nanotweezers to closed state, which leads formation in presence hemin produce highly sensitive...

Sugar and spice: The title method for the simultaneous multiplex analysis of intact cell-surface glycans (see picture) shows excellent performance in sensitivity, stability, practicality. strategy can be used to analyze dynamic variation glycome decipher cellular pathophysiological processes. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by authors. Please...

A rapid and sensitive strategy for the detection of Salmonella was proposed by integrating simple DNA extraction, specific polymerase chain reaction (PCR) with an invA gene-based electrochemical sensor. The amplified target sequence gene could be specifically captured on sensing interface, further hybridized biotinylated probe to form a sandwich-type hybridization structure. signal streptavidin-alkaline phosphatase (ST-AP), producing enzyme-catalyzed sensing. fabrication processes were...

<h3>Background</h3> Interleukin 27 (IL-27) is an important cytokine regulating host immune responses. However, its role in sepsis-induced immunosuppression remains unclear. <h3>Aim</h3> To investigate the of IL-27 modulating using a murine model caecal ligation and puncture (CLP)-induced sepsis followed by secondary challenge with <i>Pseudomonas aeruginosa</i>. <h3>Methods</h3> CLP or sham surgery was performed wild-type (WT) receptor (IL-27R)/WSX-1 knockout (KO) mice, then mice were...

Summary Progranulin ( PGRN ) is a multi‐functional protein known to be involved in inflammation. Recent studies have found that has dual roles inflammation and exerts anti‐inflammatory pro‐inflammatory function different diseases. However, the role of psoriasis not been fully elucidated. Here, we detected preferential expression human psoriatic lesions serum. Moreover, serum /tumour necrosis factor‐ α ratio was negatively correlated with disease severity. To investigate pathogenesis...

Abstract Transcriptional regulation of capsule expression is critical for pneumococcal transition from carriage to infection, yet the underlying mechanism remains incompletely understood. Here, we describe capsular polysaccharide, one most important virulence factor by a GntR family regulator, CpsR. Electrophoretic mobility-shift assays have shown direct interaction between CpsR and cps promoter ( cpsp ), their could be competitively interfered glucose. DNase I footprinting localized binding...

Uracil metabolism is an important step in the growth and of Streptococcus pneumoniae, pyrimidine nucleotides play role expression production S. pneumoniae capsules. MgaSpn(spd_1587),as a transcriptional ragulator host environment adaptation, regulates biosynthesis capsules phosphorylcholine. However, underlying regulation mechanism between uracil remains incompletely understood. Here, we first described relationship capsule via pyrR gene(spd_1134) pneumoniae. Electrophoretic mobility-shift...

Interaction between virulence factors of streptococcus pneumoniae and innate immune receptors elicits host responses through specific signaling pathways during infection. Insights into the events may provide a better knowledge starting for host-pathogen interaction. Here we demonstrated significant induction response elicited by recombinant endopeptidase O (rPepO), newer pneumococcal protein, both in vivo vitro. Intratracheal instillation rPepO protein resulted increase cytokines production...

Understanding of the mechanism underlying pathogenesis or protection for influenza-Streptococcus pneumoniae coinfected pneumonia provides potential strategy in decreasing high morbidity and mortality this infectious disease. Interleukin-6 (IL-6) is an important cytokine to limit infection-related inflammation; however, its role remains unclear. Here we show that patients with displayed dramatically elevated IL-6 levels; similarly, clinically relevant mice had increased production lung...

Pneumococcal polysaccharide-based vaccines are effective in preventing pneumococcus infection; however, some drawbacks preclude their widespread use developing and undeveloped countries. Here, we evaluated the protective effects of ATP-dependent caseinolytic protease (ClpP), pneumolysin mutant (DeltaA146 Ply), putative lipoate-protein ligase (Lpl), or combinations thereof against pneumococcal infections mice. Vaccinated mice were intraperitoneally and/or intranasally challenged with...

ABSTRACT Pneumolysin (Ply) and its variants are protective against pneumococcal infections in animal models, as a Toll-like receptor 4 agonist, pneumolysin has been reported to be mucosal adjuvant. DnaJ approved useful candidate vaccine protein; we therefore designed novel fusion proteins of with form Ply that deletion A146 (ΔA146Ply-DnaJ [the C terminus ΔA146Ply connected the N DnaJ] DnaJ-ΔA146Ply ΔA146Ply]) test whether they focal lethal their potential mechanisms. The purified were used...

Sepsis is a devastating condition with high mortality rate and limited treatments. characterized by failed host immune response to contain the infection, resulting in organ dysfunction. Interleukin-34 new cytokine involved infection immunity. Whether interleukin-34 beneficial or deleterious sepsis underlying mechanisms remains unknown.Prospective randomized animal investigation vitro studies.Research laboratory at university hospital.Wild-type C57BL/6 mice were used for vivo studies, septic...

The osmotic response element-binding protein (OREBP), also known as tonicity enhancer-binding (TonEBP) or NFAT5, is the only osmo-sensitive transcription factor that mediates cellular adaptations to extracellular hypertonic stress. Although it well documented subcellular localization and transactivation activity of OREBP/TonEBP are tightly regulated by tonicity, molecular mechanisms involved remain elusive. Here we show nucleocytoplasmic trafficking dual phosphorylation Ser-155 Ser-158....

A simple scanometric strategy was developed for ultrasensitive assay of matrix metalloproteinases based on their discriminatory and proteolytic activity by integrating a newly designed peptide–gold nanoparticle probe, nitrilotriacetic acid modified chip silver signal amplification.

Pneumolysin (Ply), a major virulence factor of Streptococcus pneumoniae (S. pn) , affects the immunity host cells during infection. It has been reported that Ply is involved in S. pn standard strain D39‐induced interferon‐β ( IFN ‐β) expression; however, other findings suggest recombinant protein incapable triggering ‐β expression. Here, we demonstrated purified was capable initiating oxidative damage to mitochondria, resulting subsequent release mitochondrial deoxyribonucleic acid (mt DNA...

Natural transformation plays an important role in the formation of drug-resistant bacteria. Exploring regulatory mechanism natural can aid discovery new antibacterial targets and reduce emergence Competence is a prerequisite Streptococcus pneumoniae, which comCDE operon core regulator competence. To date, only ComE has been shown to directly regulate transcription. In this study, transcriptional regulator, catabolite control protein A (CcpA), was identified that regulated We confirmed CcpA...

A pragmatic and simple strategy was developed for ultrasensitive detection of protein with good specificity low matrix effect, which combined aptamer-initiated rolling circle amplification a Au nanoparticle probe convenient scanometric readout.

A novel, label-free strategy has been developed for facile electrochemical analysis of dynamic glycan expression on living cells, which uses carbon nanohorns to efficiently immobilize lectin the construction a recognition interface and enhancing accessibility cell surfaceglycan motifs.