A5101819625- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Immune Response and Inflammation
- Streptococcal Infections and Treatments
- Respiratory viral infections research
- Pneumocystis jirovecii pneumonia detection and treatment
- interferon and immune responses
- Tuberculosis Research and Epidemiology
- Influenza Virus Research Studies
- Mycobacterium research and diagnosis
- Asthma and respiratory diseases
- Mast cells and histamine
- RNA and protein synthesis mechanisms
- Inflammasome and immune disorders
- High Altitude and Hypoxia
- Diet, Metabolism, and Disease
- Antimicrobial Resistance in Staphylococcus
- Immune Cell Function and Interaction
- Ocular Diseases and Behçet’s Syndrome
- Immunodeficiency and Autoimmune Disorders
- Glycosylation and Glycoproteins Research
- Gestational Trophoblastic Disease Studies
- Food Allergy and Anaphylaxis Research
- Peptidase Inhibition and Analysis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Chongqing Medical University
2012-2022
Children's Hospital of Chongqing Medical University
2008-2019
Pneumococcal polysaccharide-based vaccines are effective in preventing pneumococcus infection; however, some drawbacks preclude their widespread use developing and undeveloped countries. Here, we evaluated the protective effects of ATP-dependent caseinolytic protease (ClpP), pneumolysin mutant (DeltaA146 Ply), putative lipoate-protein ligase (Lpl), or combinations thereof against pneumococcal infections mice. Vaccinated mice were intraperitoneally and/or intranasally challenged with...
Summary Airway inflammatory diseases such as chronic obstructive pulmonary disease (COPD) and asthma are associated with elevated expression of interleukin‐32 (IL‐32), a recently described cytokine that appears to play critical role in inflammation. However, so far, the regulation IL‐32 production has not been fully established. We examined by tumour necrosis factor‐ α (TNF‐ ) primary human lung fibroblasts. Human fibroblasts were cultured presence or absence TNF‐ and/or other...
Streptococcus pneumoniae (S. pn), the bacterial pathogen responsible for invasive pneumococcal diseases, is capable of producing substantial amounts hydrogen peroxide. However, impact S. pn-secreted peroxide (H2O2) on host immune processes not completely understood. Here, we demonstrated that H2O2 caused mitochondrial damage and severe histopathological in mouse lung tissue. Additionally, only oxidative to deoxyribonucleic acid (mtDNA), but also a reduction mtDNA content alveolar epithelia...
Pneumolysin (Ply), a major virulence factor of Streptococcus pneumoniae (S. pn) , affects the immunity host cells during infection. It has been reported that Ply is involved in S. pn standard strain D39‐induced interferon‐β ( IFN ‐β) expression; however, other findings suggest recombinant protein incapable triggering ‐β expression. Here, we demonstrated purified was capable initiating oxidative damage to mitochondria, resulting subsequent release mitochondrial deoxyribonucleic acid (mt DNA...
Oleanolic acid (OA), contained in more than 1620 plants and as an aglycone precursor for naturally occurred synthesized triterpenoid saponins, is used China liver disorders humans. However, the underlying liver-protecting mechanisms remain largely unknown. Here, we found that treatment of rats with OA (25 mg/kg/day, gavage, once daily) over 10 weeks diminished liquid fructose-induced excess hepatic triglyceride accumulation without effect on total energy intake. Attenuation increased...
Zinc metalloprotease B (ZmpB) is present in all isolated pneumococcal strains and contributes to the pathogenesis of infection. In this study, recombinant ZmpB was cloned expressed Escherichia coli. The expression by different detectable Western blotting with antisera raised ZmpB. Flow cytometry analysis demonstrated that anti-ZmpB polyclonal antibodies could bind cell surface analyzed. Both protein significantly inhibited adhesion Streptococcus pneumoniae D39 A549 cells. mouse models,...
Two-component systems (TCSs) have the potential to be an effective target of antimicrobials, and thus received much attention in recent years. VicK/VicR is one TCSs Streptococcus pneumoniae (S. pneumoniae), which essential for pneumococcal survival. We previously obtained several Traditional Chinese Medicine monomers using a computer-based screening. In this study, either alone or combination with penicillin, their antimicrobial activities were evaluated based on vivo vitro assays. The...
We have recently reported that neutrophils play a pivotal role in innate defense against Streptococcus pneumoniae ( Spn) during mouse acute otitis media (AOM). However, the underlying mechanism remains unclear. By constructing models of pneumococcal AOM C57BL/6 mice and using specific inhibitor vivo, we investigated myeloperoxidase (MPO), one most important protein components neutrophils. Experiment results showed significant increase MPO production recruited Spn-infected mice. Neutrophils...
BackgroundMast cells participate in immune responses by releasing potent system modifiers via degranulation. Due to currently reported controversial roles of mast Streptococcus pneumoniae infections, this study aimed determine the role and mechanism clearing S. mice.MethodsIn vivo mouse model cell degranulation established administration C48/80 was evaluated for influences on bacterial colonization inflammation. In vitro observe phagocytic bactericidal functions neutrophils macrophages. IL-6...
ABSTRACT Mucosal immunization with attenuated vaccine can protect against pneumococcal invasion infection, but the mechanism was unknown. Our study found that mucosal delivery live SPY1 strain confer T cell- and B cell-dependent protection colonization invasive infection; yet it is still unclear which cell subsets contribute to protection, their roles in remain elusive. Adoptive transfer of anti-SPY1 antibody conferred naive μMT mice, immune cells were indispensable examined nude mice. A...
Summary Invasive pneumococcal diseases incur significant mortality, morbidity and economic costs. The most effective strategy currently available to reduce the burden of these is vaccination. In this study, we evaluated protective efficacy immunizing mice with caseinolytic protease (ClpP) protein antigen whose gene sequences were shown be highly conserved in different strains Streptococcus pneumoniae an invasive-disease model (intraperitoneal infection model), protection against invasive...
Interaction between pneumococcal virulence factors and innate immune receptors triggers host responses via specific signaling pathways after infection. By generating a deficient mutant, we show here that, compared with the wild-type parent strain, glycosyl hydrolase 25 relating to invasion protein (GHIP) mutant strain was impaired in rapid dissemination into vessels caused less severe inflammation mice lungs. Further study demonstrated that lack of this Streptococcus pneumoniae an increased...
The paper is about rapid screening of tuberculosis for detecting mts90, a new molecular target of<italic>Mycobacterium tuberculosis</italic>.
Acute otitis media (AOM) is one of the most common bacterial infectious diseases in children aged 2 to 7 years worldwide. We previously demonstrated that interleukin-17A (IL-17A) promotes an acute inflammatory response characterized by influx neutrophils into middle ear cavity during Streptococcus pneumoniae-induced AOM. In general, viewed as effector frequently causes local tissue damage. However, little known about pathogenic effects IL-17A Here, we investigated using wild-type (WT) and...
Streptococcus pneumoniae is a major pathogen leading to substantial morbidity and mortality in children under 5 years of age. Vaccination an effective strategy prevent S. infection. SPY1 pneumococcal vaccine candicate strain obtained our previous study. To improve its stability immunogencity, this study, we constructed the SPY1△lytA which lacks autolysin activity was coated with artificial exterior surface calcium phosphate shell by situ mineralization. The resulting SPY1△lytACaPi displayed...
Increasing evidences demonstrate that microorganism and their products protect against bacterial viral pathogens through various mechanisms including immunomodulation. Streptococcus pneumoniae endopeptidase O (PepO), a pneumococcal virulence protein, has been proven to enhance the phagocytosis of Staphylococcus aureus by macrophages in our previous study, where we detected down regulation SH2 domain-containing inositol phosphatase 1 (SHIP1) up complement receptor 3 (CR3) PepO-stimulated...