A5100743201- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Antimicrobial Resistance in Staphylococcus
- Bacterial Genetics and Biotechnology
- interferon and immune responses
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- RNA and protein synthesis mechanisms
- Bacterial biofilms and quorum sensing
- Bacterial Infections and Vaccines
- Immune Cell Function and Interaction
- Pneumonia and Respiratory Infections
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Streptococcal Infections and Treatments
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Salmonella and Campylobacter epidemiology
- Biochemical and Structural Characterization
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Animal Virus Infections Studies
Beijing Institute of Education
2010-2024
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2012-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2024
Zhuhai People's Hospital
2024
Jinan University
2024
Huzhou Women and Children's Hospital
2024
Twelfth Guangzhou City People's Hospital
2024
Peking Union Medical College Hospital
2022-2024
Shanxi Medical University
2023
ZheJiang Academy of Agricultural Sciences
2022-2023
Increasing evidence has demonstrated a significant role of long non-coding RNAs (lncRNAs) in diverse biological processes, and many which are likely to have functional roles vascular remodeling. However, their functions pulmonary arterial hypertension (PAH) remain largely unknown. Pulmonary remodeling is an important pathological feature PAH, leading increased resistance reduced compliance. artery smooth muscle cells (PASMCs) dysfunction involved Long noncoding potential regulators PASMCs...
To investigate why the level of Lyn is significantly decreased in B cells from a majority patients with systemic lupus erythematosus (SLE) and to determine role microRNA-30a (miR-30a) SLE cell hyperactivity.Luciferase reporter gene assays were performed identify interaction between miR-30a 3'-untranslated region (3'-UTR) Lyn. Levels determined by TaqMan quantitative polymerase chain reaction (qPCR), messenger RNA levels tested real-time qPCR, protein using Western blotting. The quantity IgG...
The Hfq protein is reported to be an RNA chaperone, which involved in the stress response and virulence of several pathogens. In E. coli, can mediate interaction between some sRNAs their target mRNAs. But it controversial whether plays important role S. aureus. this study, we found that deletion hfq gene aureus 8325-4 increase surface carotenoid pigments. mutant was more resistant oxidative but pathogenicity reduced. We reveal detected only strains. Using microarray qRT-PCR, identified 116...
Vasorin (VASN) is a type I transmembrane protein that plays important roles in tumor development and vasculogenesis.In this paper, we showed VASN could be key mediator of communication between cells endothelial cells.We confirmed for the first time HepG2-derived can transferred to human umbilical vein (HUVECs) via receptor mediated endocytosis exosomes, at least part through HSPGs.The containing exosomes promote migration recipient HUVECs cells.Our results identify novel pathway by which...
Valosin containing protein (VCP)/p97 plays various important roles in cells. Moreover, elevated expression of VCP hepatocellular carcinoma (HCC) is correlated with increased incidence recurrence. But the role HCC progression vitro and vivo unclear. And there are few reports about regulation mechanism on HCC. In this study, it was identified that level frequently human tissues. addition, down-regulation siRNAs could dramatically suppress genesis tumor vivo. It found miR-129-5p directly...
Long noncoding RNAs (lncRNAs) have been discovered to be playing important role in various biological processes. However, the contribution of lncRNAs pulmonary artery hypertension (PAH) remains largely unknown. Pulmonary vascular remodeling is an pathological feature PAH, leading increased resistance and reduced compliance. Here, we investigated PAH. Differences mRNAs between hypoxia PAH rats normoxia were screened using microarray analysis. The results showed that 36 519 upregulated...
Abstract Disseminated Cryptococcosis infection typically occurs in immunocompromised patients, often manifested as pneumonia or meningoencephalitis. Cases with involvement of either prostate adrenal glands are less frequent. We describe a case an 62-year-old man new-found Idiopathic CD4 + T lymphocytopenia who presented urinary irritation symptoms followed by headache. The patient was finally diagnosed disseminated cryptococcosis prostate, gland the help combining histopathology...
Neddylation is a ubiquitination-like pathway that controls cell survival and proliferation by covalently conjugating NEDD8 to lysines in specific substrate proteins. However, the physiological role of neddylation mammalian metabolism remains elusive, no mitochondrial targets have been identified. Here, we report mouse models with liver-specific deficiency or ubiquitin-like modifier activating enzyme 3 (UBA3), catalytic subunit NEDD8-activating enzyme, exhibit neonatal death spontaneous fatty...
In this study, the CAR-like multivalent aptamer nanoparticles (X-polymers) were assembled with dimer of murine CD28 RNA (CD28Apt7), tetramer CTLA-4 (cytotoxic T-lymphocyte-associated protein 4) (Del60), and a folic acid labeled ssDNA fragment in stable nucleic three-way junction scaffold (3WJ). Results showed that X-polymers could recognize both mCD28 mCTLA-4 molecules. Confocal imaging flow cytometry assays target T cells B16 vitro. With first costimulatory signals provided by CD3...
Staphylococcus aureus is a gram-positive pathogen responsible for wide variety of diseases. RNAIII the key effector accessory gene regulator (agr) system. It regulatory RNA (514 nucleotides long) that acts at both transcription and translation level to regulate production numerous toxins, enzymes cell surface proteins. Here, we reveal map (major histocompatibility complex class II analogous protein) positively regulated by RNAIII. Our further study indicates 108–135 nt fragment as an...
// Shaohua Li 1,* , Hui Xiqin Yang Wei Wang 1 Aixue Huang Jie Xingliang Qin Fei 2 Guanyi Lu Hongmei Ding Xueting Su Lvbin Hou Xia Ming Shi Hongwen Zhang 3 Qiang Zhao Dong Xingfeng Ge Leqiao Sun Chenjun Bai Chaonan Xuelian Shen Tao Fang Fusheng 4 Heqiu and Ningsheng Shao Beijing Institute of Basic Medical Sciences, Beijing, China Pharmacology Toxicology, Department Interventional Radiology, General Hospital Fuzhou, Center Infectous Disease, 302 Hospital, * These authors contributed equally to...
Steady-state extramedullary hematopoiesis during adulthood is an emerging field of great interest. The meninges contain both innate and adaptive immune cells, which provide immunosurveillance the central nervous system (CNS). Hematopoietic progenitors that give rise to meningeal cells remain elusive. Here, we report steady-state adult mice host hematopoietic stem (HSCs), as defined by long-term, efficient, multi-lineage reconstitution self-renewal capacity in meninges, blood, spleen, bone...
Neddylation, an important type of post-translational modification, has been implicated in innate and adapted immunity. But the role neddylation immune response against RNA viruses remains elusive. Here we report that promotes virus-induced I IFN production, especially IFN-α. More importantly, myeloid deficiency UBA3 or NEDD8 renders mice less resistant to virus infection. Neddylation is essential for virus-triggered activation Ifna gene promoters. Further exploration revealed mammalian...
The transcription factor SHOX2 gene is critical in regulating expression and the development of tumors, but its biological role prostate cancer (PCa) remains unclear. In this study, we found that was significantly raised PCa tissues associated with clinicopathological features as well disease-free survival (DFS) patients. Phenotypic tests showed absence inhibited growth invasion, while overexpression promoted these effects. Mechanistically, to activate nephronophthisis type 4 (NPHP4), a...
SsrA RNA (small stable A), also known as tmRNA and 10Sa RNA, functions both tRNA mRNA through its unique structure. The carotenoid pigment is the eponymous feature of human pathogen Staphylococcus aureus . Here, we found that mutant strain with ssrA deletion was increased. Furthermore, it demonstrated could act an antisense aside from well‐known biological function, crtMN , encoding two essential enzymes for synthesis, identified target ssrA. Further investigation showed specifically base...
Abstract LytM is one kind of autolysin expressed in Staphylococcus aureus . It can hydrolyze peptide bond pentaglycine, which the component cell wall. Until now, regulation on expression still unclear. Here, we found that level RNAIII mutant was increased. an important small regulatory RNA S. Our further investigation revealed interact with 5′UTR lytM mRNA and block ribosome binding sites [RBS]. So our results identified new target RNAIII. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Intracranial abscesses are associated with high mortality. Staphylococcus aureus is one of the main pathogens that cause intracranial infection. Until now, there no report to identify key effectors S. during infection.The murine model induced by was constructed. The vital sign and survival rate mice were observed evaluate Histological examination used diagnose pathological alterations mouse tissues. sensitivity whole blood evaluated whole-blood killing assay.In model, it shown mortality...
Fulminant hepatitis (FH) is a life-threatening disease with partially understood pathogenesis.It has been demonstrated that myeloid-derived suppressor cells (MDSCs) are recruited into the liver during this process, and their augmented accumulation by various strategies protects against injury.However, underlying mechanism(s) remain elusive.Receptor for activated C kinase 1 (RACK1), multi-functional scaffold protein, highly expressed in normal implicated physiology diseases, but vivo role of...
Staphylococcus aureus is a major human pathogen. Pathogenic effects are largely due to production of bacterial toxins, whose synthesis controlled by an mRNA molecule termed RNAIII. The S. protein called RAP (RNAIII-activating protein) secreted and activates RNAIII inducing the phosphorylation its target TRAP (target RAP). Antibodies have been shown suppress exotoxin in vitro, suggesting that may be useful vaccine site. Here we showed peptide TA21 was identified screening phage display...